Observation of Larval Development and Determination of Point of No Return of Barbatula nuda
WU Chen, LUO Xiaonian, DUAN Youjian, LI Jiao, XING Yuxin, JI Chenyue, LI Quansen
Key Laboratory of Applied Biology and Aquaculture of Northern Fish in Liaoning Province, College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China
Abstract：In order to explore the developmental characteristics and optimal feeding time of larval Barbatula nuda, the microscopic morphological observation of B. nuda larvae and juvenile was carried out systematically from 0 to 44 days post hatching(dph) and effects of fastingonlarval development, growth and digestive tract and the point of no return (PNR) were investigated. The results showed that the early development stage of B. nuda was divided into yolk sac larvae stage (0—4 dph), late larvae stage (5—26 dph) and juvenile stage(27—44 dph) at water temperature of 16.2—20.0℃ according to the exhaustion of yolk sac, formation of air bladder and body color. The newly hatched larvae had total length of (4.09±0.11) mm, and the yolk sac volume of (0.1176±0.0304) mm3. The initial feeding was observed in the larvae at 3 dph, and the yolk sac was completely exhausted at 5 dph, with mixed trophic of 3 days. At 27 dph, the air bladder drum can be observed clearly, and the end of notochord began to bend, signing the juvenile stage. The B. nuda had similar morphology of juvenile to that of adult at 44 dph. The result of starvation test showed that the initial feeding rate of the starvation larvae was 35%, with the maximum food ingestion rate of 90% for 2 days. Compared with feeding groups, the starvation larvae showed decrease in vitality, weakened clustering, and solitary swimming, without intestinal folds formed. The activities of pepsin and trysin were (0.54±0.07) U/mL and (58.83±13.99) U/mL in the starvation groups, respectively, significantly lower than those in the feeding groups (P<0.01). The degrowth was observed in the starved larvae at 6 dph, with a large number larvae starting to be death at 7 dph and all dead at 10 dph. It was found that the point of no return was 7—8 dph. Therefore, the optimal initial feeding may be 3—4 dph for B. nuda larvae. The findings preliminarily revealed the basic rules of early development of B. nuda and provided basic biological data for the early development biology research and feeding strategies of B. nuda.
吴晨, 骆小年, 段友健, 李姣, 邢雨忻, 季辰跃, 李权森. 北方须鳅仔稚鱼发育观察及饥饿不可逆点的确定[J]. 水产科学, 2025, 44(2): 212-222.
WU Chen, LUO Xiaonian, DUAN Youjian, LI Jiao, XING Yuxin, JI Chenyue, LI Quansen. Observation of Larval Development and Determination of Point of No Return of Barbatula nuda. Fisheries Science, 2025, 44(2): 212-222.
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2025, Vol. 44 
