Abstract：Juvenile rainbow trout with body length of 7.6—8.9 cm were fed the diets containing Chinese herbal medicine compound prepared by jujube, yam and astragalus extracts (20∶1) in equal proportion at a dose of 0%, 0.3%, 0.6% and 1.2% for 56 d, and then were injected with Vibrio parahemolyticus (5.48×106 cfu/ind). Four days after injection, the expressions of antioxidant related genes of GST, HSP90BB, CAT, Gpx1, and SOD in intestine were detected in the rainbow trout challenged by V. parahemolyticus by using common qPCR and RT-qPCR techniques to probe into the molecular mechanism of resistance to pathogenic bacteria in rainbow trout fed the Chinese herbal medicine compound. The results showed that the expression levels of GST, CAT and HSP90BB genes were significantly up-regulated (P<0.01) in 0.3% experimental group, and GST and CAT genes were significantly up-regulated (P<0.05, P<0.01) in 0.6% experimental group, while SOD gene was found to be significantly down-regulated in 0.3% and 0.6% experimental groups. The findings indicated that the expressions levels of immune-related genes were affected in the rainbow trout fed compound Chinese herbal medicine additives (jujube+yam+astragalus) infected with V. parahemolyticus, and with the significantly regulated immune function.
[1]谢谊,周其椿,梁正其. 1例虹鳟烂鳃病的细菌学诊断[J].贵州畜牧兽医,2013,37(2):66-68. [2]张耀武.虹鳟弧菌病诊治一例[J]. 科学养鱼,2015(6):65. [3]Mohan K N, Ravichandran S, Muralisankar T, et al. Application of marine-derived polysaccharides as immunostimulants in aquaculture:a review of current knowledge and further perspectives[J]. Fish & Shellfish Immunology,2019,86:1177-1193. [4]陈丽婷,郇志利,王晓清,等. 中草药添加剂在水产养殖中的应用研究进展[J]. 水产科学,2014,33(3):190-194. [5]孙裔雷,王荻,刘红柏.复方中草药对虹鳟抗氧化能力的影响[J].大连海洋大学学报,2015,30(2):170-174. [6]苏如玉,张磊,张玉,等.从药物代谢动力学角度探讨中药复方有效性的思路与方法[J].中国中西医结合杂志,2016,36(1):111-115. [7]沈兆媛,鲁建飞,许丹,等. 中草药饲料添加剂对异育银鲫感染Ⅱ型鲤疱疹病毒的影响[J]. 水产科学,2018,37(3):289-294. [8]李思明,丁惠君,郭小泽,等. 复方中草药对中华绒螯蟹生长、非特异性免疫和抗病力的影响[J].水产科学,2015,34(4):201-207. [9]梁拥军,孙向军,孙砚胜,等.复方中草药对宝石鲈抗氧化能力及免疫功能的影响[J].安徽农业科学,2009,37(15):7026-7029. [10]李小梅,劳惠燕,王蔚淼,等.复合中草药对草鱼生长性能和非特异性免疫功能的影响[J]. 粮食与饲料工业,2013(12):49-51. [11]周永昌,姜晓东,龙晓文,等. 中草药对中华绒螯蟹幼蟹生长及免疫力的影响[J]. 水产科学,2019,38(2):188-193. [12]黄海英.大枣提取液增强免疫力的研究[J]. 人人健康,2016(16):1,39. [13]Wang F, Liu F, Wang X J, et al. Transcriptomic responses to yam (Dioscorea oppositifolia L.) extract dietary supplementation in rainbow trout (Oncorhynchus mykiss) liver[J]. Aquaculture Research,2020,51(3):932-945. [14]贾睿,杜金梁,曹丽萍,等.黄芪提取物对鲤急性肝组织损伤的保护作用[J]. 江苏农业学报,2013,29(3):606-612. [15]王宁,崔一喆,蒋剑成,等.黄芪提取物对常见致病菌的体内外抑菌活性测定[J]. 畜牧与饲料科学,2014,35(4):1-2. [16]赵俊华,杨泽,曾卫东,等.出口水产养殖场鲜活淡水鱼和塘水中致病性弧菌的调查研究[J]. 旅行医学科学,2004,10(2):1-3. [17]侯粲.虹鳟中几种色素的沉积规律与产品真实性识别方法研究[D].北京:中国农业科学院,2013. [18]Capkin E, Ozcelep T, Kayis S, et al. Antimicrobial agents, triclosan, chloroxylenol, methylisothiazolinone and borax, used in cleaning had genotoxic and histopathologic effects on rainbow trout [J]. Chemosphere,2017,182:720-729. [19]Meister A. Glutathione metabolism[J]. Methods in Enzymology,1995,251:3-7. [20]贾贞,王丹,游松.谷胱甘肽的研究进展[J].沈阳药科大学学报,2009,26(3):238-242. [21]Yu L Q, Lam J C W, Guo Y Y, et al. Parental transfer of polybrominated diphenyl ethers(PBDEs) and thyroid endocrine disruption in zebrafish[J]. Environmental Science & Technology,2011,45(24):10652-10659. [22]Allocati N, Federici L, Masulli M, et al. Glutathione transferases in bacteria[J]. The FEBS Journal,2009,276(1):58-75. [23]Abdel-Gawad F. Effect of polycyclic aromatic hydrocarbons (PAHs) on modulate genes encoding stress related proteins and antioxidant enzymes in different marine fish species of Red Sea water[J]. World Applied Sciences Journal,2014,32(12):2337-2347. [24]Jin Y X, Zhang X X, Shu L J, et al. Oxidative stress response and gene expression with atrazine exposure in adult female zebrafish (Danio rerio)[J]. Chemosphere,2010,78(7):846-852. [25]王海阳,黄郁葱,丁燏,等.哈维氏弧菌GST核酸疫苗制备及其对斜带石斑鱼的免疫保护作用[J].广东海洋大学学报,2014,34(4):50-55. [26]张军民.谷氨酰胺对早期断奶仔猪肠道的保护作用[D].北京:中国农业科学院,2000. [27]白东清,高妍,刘宏超,等. 三种复方中草药对丁鱥抗氧化和非特异性免疫力的影响[G]// 第九届世界华人鱼虾营养学术研讨会论文集.厦门:中国水产学会,2013:278. [28]刘红柏,宿斌,王荻.中草药方剂对哲罗鱼抗氧化能力的影响[J].东北农业大学学报,2012,43(9):127-134. [29]Basu N, Todgham A E, Ackerman P A, et al. Heat shock protein genes and their functional significance in fish[J]. Gene,2002,295(2):173-183. [30]Pirkkala L, Alastalo T P, Zuo X, et al. Disruption of heat shock factor 1 reveals an essential role in the ubiquitin proteolytic pathway[J]. Molecular and Cellular Biology,2000,20(8):2670-2675. [31]周彦静.持续热应激下虹鳟热应激蛋白基因HSP90α和HSP10 mRNA表达、组织损伤及血细胞凋亡变化[D].兰州:甘肃农业大学,2017. [32]林天势,薛良义,孙爱飞,等.温度和病原菌感染对大黄鱼热激蛋白90基因表达的影响[J]. 中国细胞生物学学报,2012,34(6):555-564.
2020, Vol. 39 
