Vibration Sensitivity and Suitable Hatching Mode of Schizothorax waltoni Eggs
ZENG Benhe1, LIU Haiping1, Wangjiu1, MU Zhenbo1, WANG Wanliang1, WANG Jinlin1, ZHOU Jianshe1, ZHU Chengke2, YANG Ruibin3
1. Institute of Fisheries Science, Xizang Autonomous Region Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850002, China; 2. Key Laboratory of Aquatic Organism Reproduction and Development, Ministry of Education, Key Laboratory of Fishery Sciences of Chongqing, Department of Fisheries in Rongchang Compus, Southwest University, Chongqing 402460, China; 3. College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China
Abstract：The fertilized eggs (with fertility of 91.23%, 64.26% and 45.65%) of Schizothorax waltoni derived from the same batch of eggs were randomly divided into three parts and incubated in three types of incubators to compare three incubation patterns of S. waltoni eggs and the embryos of different developmental stages were oscillated at frequency of 0, 50 r/min (0.157 m/s), 100 r/min (0.314 m/s), 150 r/min (0.471 m/s), 200 r/min (0.628 m/s) and 250 r/min (0.785 m/s) for 5 minutes to explore the sensitivity of the embryos to shock. The results showed that the descending order of hatching rate was expressed as horizontal incubator (fertilization rate of 91.23%, 64.26%, and 45.65%; hatching rate of 81.74%, 40.30%, and 6.08%)>vertical incubator (91.23%, 64.26%, and 45.65%; 32.77%, 2.59%, and 0)>cylindrical incubator (91.23%, 64.26%, and 45.65%; 5.42%, 0, and 0). The minimal mortality [(0.22±0.15)%] was found in the embryos exposed to flowing rate of 0, without significantly different from that in the embryos exposed to flowing rate of 0.157—0.471 m/s, There was no significant difference in themortality of fertilized eggs between groups with flowing rates of 0.157—0.628 m/s. The maximal mortality was found in the 0.785 m/s group, which was significantly higher than that in the other groups. Under the condition of 0—0.471 m/s, the maximal mortality [(10.00±5.61)%] was found in the late gastrula stage, followed by the multicellular stage [(5.83±2.85)%]. Under the condition of 0.157—0.628 m/s, the maximal mortality [(26.67±23.45)%] was found in the 64-celled stage, which was not significantly different from that in the late gastrula stage and multicellular stage. The minimal mortality (0) was found in the rudimental heart and heart beating stages in 0.785 m/s group,which was lower than that in the other developmental stages. S. waltoni embryos was sensitive to flowing rate at different developmental stages. It is suggested to hatch the fertilized eggs in a horizontal incubator with a low flowing rate.
2021, Vol. 40 
