Effects of Dietary Bacillus velezensis MSP05 on Growth Performance, Intestinal Digestive Capacity and Non-Specific Immune of Loach Misgurnus anguillicaudatus
MENG Wenrong1, MI Haoyu1, HUANG Yichen1, HUANG Zhifa1, LIU Qitong2, XIA Mengjie2, FANG Yaowei2
1. College of Marine Science and Aquaculture, Jiangsu Ocean University, Lianyungang 222005, China; 2. College of Food Science and Engineering, Jiangsu Ocean University, Lianyungang, 222005, China
Abstract：This study was conducted to investigate the effect of dietary Bacillus velezensis MSP05 on growth performance, digestion, and non-specific immune function of juvenile loach Misgurnus anguillicaudatus. 360 fishes with an average initial body weight of (3.24±0.14) g were randomly assigned into 4 groups in triplicates with 30 individuals per replicate. One control group and three experimental groups were added 0, 1×106, 1×107, 1×108 cfu/g B. velezensis MSP05 to basal diets respectively. After 8 weeks of feeding, the results demonstrated that the weight gain rate(WGR) and specific growth rate(SGR) of the groups adding B. subtilis MSP05 were significantly higher than those of the control group (P<0.05). The activities of protease and amylase increased firstly and then decreased with the B. velezensis level increasing, achieving the highest value in 1×107 cfu/g group, while the activities of lipase rose continuously. The optimal morphology of foregut, midgut and hindgut of loach were observed in 1×106, 1×106, 1×107 cfu/g group, respectively. The activities of serum catalase (CAT), superoxide dismutase (SOD), lysozyme (LSZ) and alkaline phosphatase (ALP) in the serum were significantly higher than the control group (P<0.05), while MDA levels showed the opposite trend. Challenged by Aeromonas hydrophila for 15 days, the cumulative mortality rate of juvenile loaches decreased significantly fed with B. velezensis. In conclusion, the addition of B. velezensis MSP05 can significantly enhance the growth performance, digestive enzymes activity, intestinal morphology, antioxidant enzyme activity, non-specific immune function and disease resistance against A. hydrophila.
[1]王秋菊,崔一喆.微生态制剂及其应用[M].北京:化学工业出版社,2014. [2]RUIZ-GARCíA C, BÉJAR V, MARTíNEZ-CHECA F, et al. Bacillusvelezensis sp. nov., a surfactant-producing bacterium isolated from the river Vélez in Málaga, southern Spain[J].International Journal of Systematic and Evolutionary Microbiology,2005,55(1):191-195. [3]WANG L T, LEE F L, TAI C J, et al. Bacillus velezensis is a later heterotypic synonym of Bacillus amyloliquefaciens[J].International Journal of Systematic and Evolutionary Microbiology,2008,58(Pt 3):671-675. [4]BORRISS R, CHEN X H, RUECKERT C, et al. Relationship of Bacillus amyloliquefaciens clades associated with strains DSM 7T and FZB42T:a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp.plantarum subsp.nov.based on complete genome sequence comparisons[J].International Journal of Systematic and Evolutionary Microbiology,2011,61(8):1786-1801. [5]CAI X C, LIU C H, WANG B T, et al. Genomic and metabolic traits endow Bacillus velezensis CC09 with a potential biocontrol agent in control of wheat powdery mildew disease[J].Microbiological Research,2017,196:89-94. [6]邓丽,孙冉,姚蒋庞,等.贝莱斯芽孢杆菌粗提物对金黄色葡萄球菌的抑菌机制[J].食品与发酵科技,2021,57(3):1-9. [7]SILVA E F, SOARES M A, CALAZANS N F, et al. Effect of probiotic (Bacillus spp.) addition during larvae and postlarvae culture of the white shrimp Litopenaeus vannamei[J].Aquaculture Research,2012,44(1):13-21. [8]GUO X, CHEN D D, PENG K S, et al. Identification and characterization of Bacillus subtilis from grass carp (Ctenopharynodon idellus) for use as probiotic additives in aquatic feed[J].Fish & Shellfish Immunology,2016,52:74-84. [9]LI J, WU Z B, ZHANG Z, et al. Effects of potential probiotic Bacillus velezensis K2 on growth, immunity and resistance to Vibrio harveyi infection of hybrid grouper (Epinepheluslanceolatus♂×E. fuscogut-tatus ♀)[J].Fish & Shellfish Immunology,2019,93:1047-1055. [10]THURLOW C M, WILLIAMS M A, CARRIAS A, et al. Bacillus velezensis AP193 exerts probiotic effects in channel catfish (Ictalurus punctatus) and reduces aquaculture pond eutrophication[J].Aquacul-ture,2019,503:347-356. [11]WANG C, LIU Y, SUN G X, et al. Growth, immune response, antioxidant capability, and disease resistance of juvenile Atlantic salmon (Salmo salar L.) fed Bacillusvelezensis V4 and Rhodotorula mucilaginosa compound[J].Aquaculture,2019,500:65-74. [12]王金燕.贝莱斯芽孢杆菌DY-6的抑菌特性及其对刺参生长免疫和池塘菌群结构的影响研究[D].上海:上海海洋大学,2018:20-23. [13]YI Y L, ZHANG Z H, ZHAO F, et al. Probiotic potential of Bacillusvelezensis JW:antimicrobial activity against fish pathogenic bacteria and immune enhancement effects on Carassius auratus[J].Fish & Shellfish Immunology,2018,78:322-330. [14]高晓丹,马鸣超,曹凤明,等.基于生物化学和基因组学方法的Bacillusvelezensis 19573-3生防潜力评价[J].植物营养与肥料学报,2021,27(6):1068-1079. [15]CAO H Z, YU R H, ZHANG Y Y, et al. Effects of dietary supplementation with β-glucan and Bacillus subtilis on growth, fillet quality, immune capacity, and antioxidant status of Pengze crucian carp (Cara-ssius auratus var.Pengze)[J].Aquaculture,2019,508:106-112. [16]胡曦,杨红玲,孙云章.饲料中添加芽孢杆菌细胞壁对斜带石斑鱼生长、消化酶活性及免疫功能的影响[J].饲料与畜牧,2018(5):45-48. [17]李军亮,杨奇慧,谭北平,等.低鱼粉饲料添加枯草芽孢杆菌对珍珠龙胆石斑鱼幼鱼生长、消化酶活性、抗氧化酶活性及其mRNA表达的影响[J].水产学报,2019,43(4):1126-1137. [18]VERMA M, EKKA A, MOHAPATRA T, et al. Optimization of kraft lignin decolorization and degradation by bacterial strain Bacillusvelezensis using response surface methodology[J].Journal of Environmental Chemical Engineering,2020,8(5):104270. [19]钟丽娟,张庆华.一株饲用贝莱斯芽孢杆菌的分离鉴定及其生理特性[J].中国饲料,2021(11):26-29. [20]WON S, HAMIDOGHLI A, CHOI W, et al. Effects of Bacillus subtilis WB60 and Lactococcus lactis on growth, immune responses, histology and gene expression in Nile tilapia, Oreochromis niloticus[J].Microorganisms,2020,8(67):1-15. [21]HASSAAN M S, MOHAMMADY E Y, SOAUDY M R, et al. Synergistic effects of Bacillus pumilus and exogenous protease on Nile tilapia (Oreochromis niloticus) growth, gut microbes, immune response and gene expression fed plant protein diet[J].Animal Feed Science and Technology,2021,275:114892. [22]AL-DERINY S H, DAWOOD M A O, ZAID A A A, et al. The synergistic effects of Spirulina platensis and Bacillus amyloliquefaciens on the growth performance, intestinal histomorphology, and immune response of Nile tilapia (Oreochromis niloticus)[J].Aquaculture Reports,2020,17:100390. [23]李源.谷氨酰胺对泥鳅生长和非特异性免疫的影响[D].雅安:四川农业大学,2013:21-32. [24]SHEN W Y, FU L L, LI W F, et al. Effect of dietary supplementation with Bacillus subtilis on the growth, performance, immune response and antioxidant activities of the shrimp (Litopenaeus vannamei)[J].Aquaculture Research,2010,41(11):1691-1698. [25]WANG M, YI M M, LU M X, et al. Effects of probiotics Bacillus cereus NY5 and Alcaligenes faecalis Y311 used as water additives on the microbiota and immune enzyme activities in three mucosal tissues in Nile tilapia Oreochromis niloticus reared in outdoor tanks[J].Aquaculture Reports,2020,17:100309. [26]LI W F, ZHANG X P, SONG W H, et al. Effects of Bacillus preparations on immunity and antioxidant activities in grass carp (Ctenopharyngodonidellus)[J].Fish Physiology and Biochemistry,2012,38(6):1585-1592. [27]张维娜,夏飞,谢骏,等.凝结芽孢杆菌JSSW-07对异育银鲫生长及免疫功能的影响[J].江苏农业学报,2015,31(3):630-637. [28]于瑞河.益生菌蜡样芽孢杆菌对彭泽鲫生长、营养代谢、抗氧化性及炎症反应的影响[D].南昌:南昌大学,2020:34-40.
2023, Vol. 42 
