Virulence Genes and Drug Resistance of Pathogen Streptococcusagalactiae in Hainan
WANG Aiyuan1, ZHENG Lixin2, PU Wenyuan1, YANG Nuo1, ZENG Jifeng1, GUO Guiying3, LI Qian4, ZHENG Jiping1
1.Institute of Life Sciences and Pharmacy, Hainan University, Haikou 570228, China; 2.Hospital of Hainan University, Haikou 570228, China; 3.Academic Affairs Office of Hainan University,Haikou 570228, China; 4.Network and Educational Technology Center of Hainan University, Haikou 570228, China
Abstract：Virulence genes and resistance genes were screened in 18 strains of Streptococcus agalactiae as an important zoonotic and human pathogen collected from women and tilapia in 2017 via 14 virulence genes by PCR, and sequencing and amplification and drug sensitivity in Hainan was determined by bacterial drug susceptibility paper in order to investigate its virulence genes, carrying rate and drug resistance of the pathogen. The results showed that there was high positive detection rate in the virulence genes among the tested strains, detection of 11 virulence genes from all samples, with as high as 66.7% of positive rate in the remaining genes. The amplification of drug resistance genes revealed that the positive rate of the rest genes was high(44.4%—100%) except for the negative resistance genes against tetracycline and amino acid glycoside. S. agalactiae was shown to have high tolerance to β-lactams, macrolides, and lincosamides and high sensitivity to the amino acid glycoside, quinolone, and tetracycline, indicating that the drug sensitivity is consistent with the detection of drug resistance genes. Therefore, it should be concerned about and judged the high frequency of virulence genes and the high drug resistance among S. agalactiae isolated in Hainan in traditional antibiotic treatment.
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2020, Vol. 39 
