Transcriptome on Response of Four Species of Green Tide Algae to Heat Stress
ZHAO Hui1, ZHOU Lingjie1, CAI Chun′er1,2, HE Peimin1,2
1. College of Oceanography and Ecological Science, Shanghai Ocean University, Shanghai 201306, China; 2. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
Abstract：The Yellow Sea green tide has seriously damaged the marine ecology and caused huge economic loss and serious social influence. Four species of green tide algae Ulva prolifera, U. linza, U. flexuosa, and U. compressa were collected from the coast of Qingdao in July 2008, and the progeny remaining after pure line culture under suitable conditions were divided into two groups: the control group was treated at the optimum temperature and the treatment group was treated at 30 °C for 24 h. The algae were taken out, treated with liquid nitrogen and stored at -80 °C until RNA extraction, RNA quality control detection and transcriptome by high-throughput sequencing. It was found that the descending order of the number of differential genes in four Ulva species was expressed as U. compressa＞U. flexuosa＞U. prolifera＞U. linza. These differential genes were enriched in four metabolic pathways of transport and catabolism, lipid metabolism, amino acid metabolism and energy metabolism, while the differential genes in U. prolifera were enriched in pathways specific to oxidative phosphorylation, glyoxylate and dicarboxylic acid metabolism and ABC transporter, which may be related to the special response of U, prolifera to high temperature stress. The four types of Ulva all had complete heat shock protein synthesis and antioxidant systems, and the down-regulation of heat shock protein-related gene expression in Ulva compressa. Different Ulva resisted high temperature through the expression of different antioxidant enzymes, in which the role of superoxide dismutase was the most important. The finding provides theoretical basis for succession of four Ulva species during the outbreak of green tide.
赵卉, 周玲洁, 蔡春尔, 何培民. 4种绿潮藻响应高温胁迫的转录组学研究[J]. 水产科学, 2024, 43(2): 199-214.
ZHAO Hui, ZHOU Lingjie, CAI Chun′er, HE Peimin. Transcriptome on Response of Four Species of Green Tide Algae to Heat Stress. 水产科学, 2024, 43(2): 199-214.
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2024, Vol. 43 
