Effects of Three Aerobic Denitrifying Bacteria on Intestinal Morphology and Microbiota of Zebrafish
ZHENG Yazhi1,2, LU Huijie2, XU Jingxuan1,2, LIU Fengkun1,2, RUAN Zhuohao2, YIN Peng2, GUO Qiang2, MA Yanping3, DAI Ruizhi4, LIAO Jinsong4, DAI Jieyu4, GUO Hui1, HUANG Wen1,2
1. College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; 2. Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Animal Breeding and Nutrition, Collaborative Innovation Center of Aquatic Sciences, Guangdong Academy of Agricultural Sciences, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; 3. Institute of Animal Health, Guangzhou 510640, China; 4. Juncheng Herui Environmental Technology Group Co., Ltd., Yunfu 527300, China
Abstract：In the experiment the effects of three aerobic denitrifying bacteria, Klebsiella oxytoca DZ9, K. oxytoca WM27, and Serratia marcescens DZ16, on the intestinal morphology and microbial community of zebrafish Danio rerio were systematically evaluated through histopathological analysis and high-throughput sequencing technology, aiming to provide safe and efficient candidate strains for aquaculture water quality modulation and wastewater treatment. A total of 360 zebrafish were randomly divided into four groups, (control and three treatment groups with triplicate biological replicates), receiving bacterial suspensions regular of 106 cfu/mL) for 30 days. The results indicated that: no significant differences in survival rates were observed between treatment and control groups, with maintained intestinal structural integrity characterized by well-arranged villus and absence of pathological lesions, indicating good biosafety. Significant improvement in several intestinal development parameters were detected, including increased villus height and goblet cell density across treatment groups (P<0.05), with K. oxytoca WM27 group′s thickening of muscular layer (P<0.05). Microbial community analysis revealed significant reduction in potentially pathogens, such as Aeromonas and Plesiomonas, concurrent with increased beneficial microbial taxa including Bradyrhizobium, Hyphomicrobium, Cetobacterium, Lactobacillus, and Reyranella (P<0.05). This microbial modulation potentially enhances intestinal barrier integrity and reduces inflammation risks through competitive colonization mechanisms. The results demonstrate that specific aerobic denitrifying strains can effectively improve intestinal morphology and microbiota composition of zebrafish, providing theoretical basis for developing microbial agents in sustainable aquaculture practices.
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2026, Vol. 45 
