Analysis of Genetic Diversity in Breeding Population of Chinese Mitten Crab Eriocheir sinensis in Changjiang River
WEI Chuangchuang1,2, LU Suwan2, ZHOU Jun2, DENG Yanfei2, ZHANG Huimin2, GU Wei1, MENG Qingguo1, LI Xuguang1,2
1. College of Marine Science and Engineering, Nanjing Normal University, Nanjing 210023, China; 2. Key Laboratory of Genetic Breeding and Cultivation for Freshwater Crustacean, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, China
Abstract：To explore the genetic diversity of breeding populations of Chinese mitten crab Eriocheir sinensis from the Changjiang River system, a fragment of the mitochondrial control-region (D-loop) gene was amplified by PCR and directly sequenced in individuals collected from five localities including Changzhou, Nanjing, Suqian, Suzhou, and Zhenjiang, Jiangsu province. The results showed that a total of 192 D-loop gene sequences (790 bp) were detected, including 625 conserved sites and 164 variable sites, with higher AT content (80.2%) than the CG content (19.8%) in the gene sequences, indicating a significant bias in base composition. A total of 40 haplotypes were detected in the five breeding populations, including 12 shared haplotypes and 28 unique haplotypes, with overall haplotype diversity index (Hd) of 0.890, and the overall nucleotide diversity index (Pi) of 0.047 30, indicating a high level of genetic diversity. The genetic distance between populations was found to be ranged from 0.030 50 to 0.072 40, and the genetic differentiation coefficient between populations ranged from -0.005 65 to 0.166 76, indicating low genetic differentiation. Analysis of molecular variance revealed that 95.10% of the genetic variation was originated within populations, and 4.90% originated between populations. Haplotype network analysis showed no correspondence between haplotypes and different populations, indicating frequent gene flow between populations. In conclusion, the breeding population of Chinese mitten crab in the Changjiang River system has a high level of genetic diversity and low genetic differentiation between populations. The finding provides reference with the assessment and protection of the genetic resources of the breeding population of Chinese mitten crab.
韦创创, 鲁苏皖, 周军, 邓燕飞, 张慧敏, 顾伟, 孟庆国, 李旭光. 长江水系中华绒螯蟹繁育群体遗传多样性分析[J]. 水产科学, 2026, 45(5): 822-828.
WEI Chuangchuang, LU Suwan, ZHOU Jun, DENG Yanfei, ZHANG Huimin, GU Wei, MENG Qingguo, LI Xuguang. Analysis of Genetic Diversity in Breeding Population of Chinese Mitten Crab Eriocheir sinensis in Changjiang River. Fisheries Science, 2026, 45(5): 822-828.
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2026, Vol. 45 
