Effects of Transport Time on Stress Injury and Immune Function of Red Swamp Crayfish Procambarus clarkii
ZHU Minli1, HU Honghui2, CHEN Huangen3, SU Shengyan1,2,4, ZHU Jian2
1. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; 2. Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; 3. Jiangsu Fishery Technology Promotion Center, Nanjing 226007, China; 4. Panjin Raoyang Agricultural Science and Technology Development Co., Ltd., Panjin 124107, China
Abstract：To investigate the effects of transport time on the cumulative survival rate, antioxidant capacity, and immune-related gene expression of red swamp crayfish Procambarus clarkia, red swamp crayfish with body weight of (8.26±2.67) g were placed in a shipping basket in a car for 0 (control), 2, 8, and 14 h of dry transportation at temperature of 20 °C. Survival, antioxidant metabolic enzyme activities, and immune-related gene indicators in hemolymph and hepatopancreas as well as changes in histological structure of hepatopancreas were investigated in the red swamp crayfish during 0, 2, 8, and 14 h of transportation. The cumulative survival rate was determined in the red swamp crayfish reared for 10 d after 0, 2, 8, and 14 h of transportation. The results showed that there was significantly higher cumulative survival rate in 2 hours than that after 8 hours and 14 hours, with the cumulative survival rate of only 2.22% in the red swamp crayfish exposed to 14 hour transportation in the 10th days. Histological observation showed that the hepatopancreas were severely damaged in the 8-hour group. There were significantly higher activities of total superoxide dismutase, catalase, acid phosphatase, glutamate-oxaloacetate transaminase and glutamate-pyruvate transaminase and content of malondialdehyde as well as total antioxidant capacity in the hepatopancreas in the crayfish in the 14-hour group than those in the control group (P<0.05). The expression levels of GPX, CuZnSOD, HSP70, Astacidin, and Lysozyme genes in the hepatopancreas were shown to be significantly higher in the crayfish in the 14-hour group than those in the control group (P<0.05). It was found that transportation time greater than 8 hours led to significantly high cumulative survival rate, damaged hepatopancreas, antioxidant ability, and expression of immune-related genes. Therefore, it is suggested that the transportation time in actual transportation should be lower than 8 hours. The findings may provide reference for research and development to improve the transportation survival rate of red swamp crayfish.
朱敏丽, 胡宏辉, 陈焕根, 苏胜彦, 朱健. 运输时间对克氏原螯虾应激损伤及免疫机能的影响[J]. 水产科学, 2025, 44(4): 633-641.
ZHU Minli, HU Honghui, CHEN Huangen, SU Shengyan, ZHU Jian. Effects of Transport Time on Stress Injury and Immune Function of Red Swamp Crayfish Procambarus clarkii. Fisheries Science, 2025, 44(4): 633-641.
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2025, Vol. 44 
