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| Detection Method of Hydrocortisone in Coelomic Fluid of Sea Cucumber Apostichopus japonicus |
| LIU Guiying, TIAN Jin, XIAO Yao, WU Jinhao, SONG Lun, GAO Ying, WANG Zhisong |
| Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China |
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Abstract In this paper, a ultra-performance liquid chromatography-linear ion trap mass spectrometry(UPLC-MS)method was established for the detection of hydrocortisone in coelomic fluid of juvenile and 3 year old sea cucumber Apostichopus japonicus. Extraction conditions and purification methods were optimized. Samples were extracted with 0.2% formic acid acetonitrile solution, and the extract was subjected to chromatographic separation on Captiva EMR-lipid solidphase extraction column. Quantification of the analytes was achieved by UPLC-MS using the external standard method, with the limit of detection (LOD) of 0.11 μg/kg, and the limit of quantification (LOQ) of 0.35 μg/kg. The average recoveries of hydrocortisone in blank samples at spiked levels of 20, 40, and 100 μg/L were ranged from 77% to 105%. This method had the advantages of small matrix interference and accurate, which can satisfy detection of hydrocortisone in sea cucumber.
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Received: 26 February 2019
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[1]马慧,柳淑芳,马骞,等. 硬骨鱼类渗透压的内分泌激素调节机制[J].水产科学,2012,31(1):55-59.
[2]李庆昌,陈小明,刘贤德.突变高温胁迫对大黄鱼血清生理指标的影响[J]. 渔业研究,2016,38(6):437-444.
[3]王文博,汪建国,李爱华,等.拥挤胁迫对草鱼非特异性免疫功能的影响[J].水产学报,2004,28(2):139-144.
[4]王文博,汪建国,李爱华,等.振荡胁迫后鲫鱼血液皮质醇和溶菌酶水平的变化[J]. 水生生物学报,2004,28(6):682-684.
[5]施慧雄,焦海峰,尤仲杰,等.船舶噪声对鲈鱼和大黄鱼血浆皮质醇水平的影响[J]. 生态学报,2010,30(14):3760-3765.
[6]胡静,叶乐,吴开畅,等.急性盐度胁迫对克氏双锯鱼幼鱼血清皮质醇浓度和Na+-K+-ATP酶活性的影响[J]. 南方水产科学,2016,12(2):116-120.
[7]姜丹莉,林雅云,吴玉波,等.草鱼、 银鲫和青鱼捕捞后的应激反应[J]. 水产学报,2016,40(9):1479-1485.
[8]宋凯,骆源,张春晓,等. 皮质醇对斜带石斑鱼原代培养肝细胞糖代谢的影响[J]. 动物营养学报,2016,28(11):3520-3527.
[9]李学梅,杨德国.操作胁迫对褐鳟血液理化及养殖水体中皮质醇的影响[J].水产研究,2018,5(3):141-147.
[10]胡静,周胜杰,杨蕊,等.亚硝酸盐胁迫对尖吻鲈稚鱼抗氧化酶活性及皮质醇的影响[J]. 海洋科学,2018,42(4):132-140.
[11]曾鹏,申江,陈天及. 休眠方式、温度和时间对离水储藏鲫鱼生理指标的影响[J].水产科学,2019,38(4):492-497.
[12]何杰,强俊,朱志祥,等.两种不同低温应激方法对吉富罗非鱼(Oreochromis niloticus)死亡率、血清皮质醇和天然免疫因子的影响[J]. 海洋与湖沼,2013,44(4):919-925.
[13]施兆鸿,张晨捷,彭士明,等.低盐胁迫对黄姑鱼(Nibea albiflora)幼鱼鳃离子调节、呼吸代谢酶和皮质醇的影响[J]. 渔业科学进展,2015,36(6):22-28.
[14]Tai S S, Welch M J. Development and evaluation of a candidate reference method for the determination of total cortisol in human serum using isotope dilution liquid chromatography/mass spectrometry and liquid chromatography/tandem mass spectrometry[J]. Analytical Chemistry,2004,76(4):1008-1014.
[15]Nakajima T, Tsuruoka Y, Kanda M, et al. Determination and surveillance of hydrocortisone and progesterone in livestock products by liquid chromatography-tandem mass spectrometry[J]. Food Additives & Contaminants:Part A,2015,32(11):1833-1841.
[16]Peraman R, Kalva B, Shanka S, et al. Analytical quality by design (AQbD) approach to liquid chromatographic method for quantification of acyclovir and hydrocortisone in dosage forms[J]. Analytical Chemistry Letters,2014,4(5):329-342.
[17]陈翊,李颖能,陈焕辉,等.不同化学发光分析系统在皮质醇检测中的性能评价与比较[J].国际检验医学杂志,2016,37(14):1949-1953.
[18]徐继林,杨家锋,严小军,等. 海洋鱼类血清游离皮质醇液质分析研究[J].分析化学,2008,36(7):905-909.
[19]Dodd A J, Ducroq D H, Neale S M, et al. The effect of serum matrix and gender on cortisol measurement by commonly used immuoassays[J]. Annals of Clinical Biochemistry,2014,51(Pt 3):379-385.
[20]Elfarhan N, Pickett A, Ducroq D, et al. Method-specific serum cortisol response to the adrenocorticotrophin test:comparison of gas chromatography-mass spectrometry and five automated immuoassays [J]. Clinical Endocrinology (Oxf),2013,78(5):673-680.
[21]周晓梦,张秀梅,李文涛.高温和低氧胁迫对两种规格刺参半致死时间及生理机能的影响[J].中国水产科学,2018,25(1):60-73.
[22]刘慧慧,张华威,魏潇,等.超高效液相色谱串联质谱法测定水产品中磺酰脲类除草剂残留量[J].分析化学,2018,46(3):386-392.
[23]Gomez Perez M L, Romero-Gonzalez R, Martinez Vidal J L, et al. Analysis of veterinary drug residues in cheese by ultra-high-performance LC coupled to triple quadrupole MS/MS[J]. Journal of Separation Science,2013,36(7):1223-1230. |
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