Abstract：A Dot-Enzyme Linked Immunosorbent Assay(Dot-ELISA)was successfully established for detection of Edwardsiella tarda found in Japanese flounder(Paralichthys olivaceus)by using nitrocellulose membrane as solid carrier. The working concentration of serum samples was 1∶800 dilution and that of the enzyme-labeled goat anti-rabbit IgG was 1∶200 dilution by chessboard assay. The samples with clear and obvious dots were judged as positive reaction. Edwardsiella tarda was positive in the Dot-ELISA, while Aeromonas sobria, A. hydrophila, Vibrio fluvialis, V.alginolyticus , V.anguillarum , Shewanella putrefaciens, Providencia alcalifaciens , Enterobacter sakazakii and Escherichia coli were negative in the assay. The trial indicated that this method was simple, specific, quick, direct, easy extention and application at basic units.
收稿日期: 2010-03-25
引用本文:
朱壮春,陈阳,郝东升,姜广建,邢朝斌,吴鹏,白崇洲,李金龙,史相国. 应用Dot-ELISA快速检测牙鲆腹水病病原的研究[J]. 水产科学, 2010, 29(3): 162-165.
ZHU Zhuang-chun,CHEN Yang,HAO Dong-sheng,JIANG Guang-jian,XING Zhao-bin,WU Peng,BAI Chong-zhou,LI Jin-long,SHI Xiang-guo. Rapid Detection of Pathogenic Bacteria of Ascites in Japanese Flounder(Paralichthys olivaceus)by Dot-ELISA. Fisheries Science, 2010, 29(3): 162-165.
