Abstract:From March 2012 to April 2014 ,260 individuals of Rhinogobio ventralis were collected from the upper reaches of Yangtze River in Yibin ,and used for artificial domestication and propagation .It was found that R .ventralis developed into maturation in artificial domestication condition and was induced to spaw n by the traditional techniques by a high dose of hormone injection at three times .In the experiment , 260 brood fish were induced to spawn ,with average success of 8 .1% ,and 98 000 fertilized eggs were obtained .R .ventralis had eggs with average diameter of (1 .80 ± 0 .10 ) mm ,with fertilization rate of 50 .5% —98 .9% ,and the egg membrane was expanded via water absorption to diameter of (6 .86 ± 0 .31) mm .The fertilized eggs were hatched in 56 hours at water temperature of 17 .6—18 .3 ℃ ,and 31 500 larvae were obtained ,with hatching rate of 26 .4% —48 .1% .The newly hatched larvae were measured (6 .57 ± 0 .42) mm in total length and started initial feeding at the age of 6—7 days .A total of 12000 juveniles with total length of (21 .23 ± 0 .70) mm was obtained in 30 day culture ,with survival rate of 38 .1% .The finding is the first successful artificial propagation on R .ventralis .