Identification of a Pathogenic Aeromonas media Isolated from Schizopygopsis younghusbandi
ZENG Benhe1, YANG Chengnian2, QIU Yulin2, WANG Jinlin1, WANG Wanliang1, WANG Jian2, ZHU Chengke2
1. Institute of Fisheries Science, Xizang Autonomous Region Academy of Agriculture and Animal Husbandry Sciences,Lhasa 850002, China; 2. Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education,Key Laboratory of Aquatic Science of Chongqing, College of Animal Science, Southwest University,Chongqing 402460, China
Abstract：To identify a pathogenic bacterium found in Schizopygopsis younghusbandi, a dominant pathogenic strain named LS-01 was isolated from the liver of the dying fish, and were identified according to gram staining, physiological, biochemical and molecular biological methods, and drug resistance was carried out meanwhile. The results showed that strain LS-01 was a short rod-shaped gram-negative bacterium and the physiological analysis based on 16S rRNA and gyrB gene revealed that the strain LS-01 was similarity of up to 99% with Aeromonas media. Artificial infection experiments showed that the strain LS-01 had median lethal concentration of 3.98×106 cfu/mL. In addition, drug sensitivity test showed that A. media LS-01 was highly sensitive to 12 drugs, such as ceftazidine, cefoperazone, levofloxacin and florfenicol.
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2021, Vol. 40 
