Growth and Reproductive Biology of Chinese Spined Loach Cobitis sinensis
YANG Jun1, HE Xingheng2, SUN Zhiyu3
1.The Forestry Bureau of Guangyuan City, Guangyuan 628000, China; 2.Sichuan Aquabase BiotechnologyLimited Company, Chengdu 610000, China; 3.Sichuan Academy of Forestry, Chengdu 610081, China
Abstract：In July 2012 and May 2013, 70 Chinese spined loach Cobitis sinensis were caught in traps and electrical fishing in Donghe River of Wangcang County, Guangyuan City, Sichuan Province and fixed in 10% formalin solution and then stored in 5% formalin solution, and another 43 samples were transported to the laboratory with plastic bag oxygenation. After routine biological measurements, the viscera of the tested fish were taken out, and the intestinal length, eviscerated weight and gonad weight were measured to investigate the growth and reproductive biology of the fish including food item analysis, male and female identification and sperm longevity. The Chinese spined loach had body length (L) of 34.92—102.85 mm, body weight (m) of 0.34—9.84 g, and eviscerated weight of 0.31—8.09 g, and was divide into 5 age classes, determined by vertebra, with dominant 2—4 age classes. The Chinese spined loach had good growth before 3 year old, low growth afterwards, with function between body length and body weight of m1=0.00001215L2.896, r2=0.939, nearly isometric growth. The relative condition factor was generally found to be less than 1, without significant difference in Fulton condition factor between female and male (P>0.05), even though smaller in males than in females. For the over 1 year old loach, however, there was slightly larger Clark condition factor in males than that in females. The Chinese spined loach had sexual ratio (♀∶♂) of 1∶1.69, and maturity at two years old age. The sperm survived for 14 seconds in distilled water, and longevity of the sperm was shown to be increased first and then decreases with the increase in NaCl concentration, with the maximal longevity (184 s) at NaCl concentration of 0.25%. The Chinese spined loach had absolute fecundity of 427—4047 eggs per female, with mean value of 1949.82 eggs, and relative fecundity of 107.56—566.09 eggs/g, with mean value of 356.07 eggs/g. The peak period of spawning was observed from mid-June to mid-August, spawning in batches, and roughly the same fecundity each batch, and the zygote is demersal and slightly viscous.
杨骏, 何兴恒, 孙治宇. 中华花鳅的生长和繁殖生物学的研究[J]. 水产科学, 2020, 39(2): 209-217.
YANG Jun, HE Xingheng, SUN Zhiyu. Growth and Reproductive Biology of Chinese Spined Loach Cobitis sinensis. 水产科学, 2020, 39(2): 209-217.
[1]丁瑞华.四川鱼类志[M].成都:四川科学技术出版社,1994:115-117. [2]陈景星,朱松泉.鳅科鱼类亚科的划分及其宗系发生的相互关系[J]. 动物分类学报,1984,9(2):201-208. [3]陈景星.中国花鳅亚科鱼类系统分类的研究[G]//鱼类学论文集.北京:科学出版社,1981:21-32. [4]陕西省动物研究所,中国科学院水生生物研究所,兰州大学生物系.秦岭鱼类志[M]. 北京:科学出版社, 1987:37-39. [5]陈毅峰, 陈咏霞. 中国鳅属鱼类的副性征、噶氏斑纹和分类整理(鲤形目:鳅科:鳅亚科)[J].动物分类学报,2005,30(4):647-658. [6]陈义雄,方力行.台湾淡水及河口鱼类志[M].台北:台湾海洋生物博物馆,1999:100. [7]陕西省水产研究所,陕西师范大学生物系.陕西鱼类志[M].西安:陕西科学技术出版社,1992:78-79. [8]胡克坚,罗琛.湘江流域中华花鳅的染色体组型[J].湖南师范大学自然科学学报,2005,28(4):67-69. [9]张觉民.内陆水域渔业自然资源调查手册[M].北京:农业出版社,1991. [10]殷名称.鱼类生态学[M].北京:中国农业出版社,1995. [11]杨骏,郭延蜀,鄢思利,等.嘉陵江中游黑尾?生物学的初步研究[J].水产科学,2014,33(8):476-482. [12]沈建忠,曹文宣,崔奕波.用鳞片和耳石鉴定鲫年龄的比较研究[J].水生生物学报,2001,25(5):462-466. [13]邓岳松,林浩然.鱼类精子活力研究进展[J].生命科学研究,1999,3(4):271-278. [14]陈新军.渔业资源与渔场学[M].北京:海洋出版社,2004:40. [15]Niewinski B C, Ferreri C P. A comparison of three structures for estimating the age of yellow perch [J]. North American Journal of Fisheries Management,1999,19(3):872-877. [16]Beamish R J, McFarlane G A. The forgotten requirement for age validation in fisheries biology[J]. Transactions of the American Fisheries Society,1983,112(6):735-743. [17]陈马康,童合一,陈兆祥,等.钱塘江几种经济鱼类的生长研究[J]. 生态学报,1984,4(2):181-187. [18]黄松钱,王也可,赵婷,等.河南地区大鳞副泥鳅和泥鳅的年龄与生长[J]. 华中农业大学学报:自然科学版,2014,33(5):93-98. [19]华元渝,阮景荣.鱼类的重量—身体维数关系的研究[J]. 水生生物学集刊,1983,8(1):45-61. [20]张伟,曾柳根,王静,等.鄱阳湖泥鳅繁殖与生长的初步研究[J].水利渔业,2006,26(1):40-42. [21]雷逢玉,王宾贤.泥鳅繁殖和生长的研究[J]. 水生生物学报,1990,14(1):60-67. [22]Wotton R J. Ecology of Teleost Fishes [M].Newyork:Chapman and Hall Ltd,1990. [23]Fang L, Chen I, Yang C, et al. The fish community of a high mountain stream in Taiwan and its relation to dam design[J]. Environmental Biology of Fishes,1993,38(4):321-330. [24]Oliva-Paterna F J, Torralva M M, Fernández-Delgado C. Age, growth and reproduction of Cobitis paludica in a seasonal stream[J]. Journal of Fish Biology,2002,60(2):389-404. [25]翟旭亮,李虹,曹豫,等.重庆地区泥鳅人工繁殖生物学研究[J].重庆水产,2012(2):34-42.
2020, Vol. 39 
