Effects of Nickel (Ni2+) on Growth, Spectral Characteristics and Phycobiliprotein Content of Alga Spirulinaplatensis
ZHAO Dengke1, LIU Hui2, ZHANG Shaobin1, CHEN Xiaoxing1, LIU Jinzhou1, XIN Qiao1
1.College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China; 2.College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China
Abstract：In order to study the effect of Ni2+ on growth of alga Spirulina platensis, the growth curve, biomass, absorption spectrum and phycobiliprotein content of the alga were determined and analyzed by adding different concentrations of Ni2+ to the culture medium. The results showed that at Ni2+ concentration of 0—0.5 μg/mL, with the increase in Ni2+ concentration, the content of phycobiliprotein increased, the growth of accelerated and the accumulated biomass of Spirulina was increased, the greatest promoting effect at 0.5 μg/mL Ni2+. When treated with 0.5 μg/mL Ni2+, the absorption spectrum of chlorophyll a in blue and purple region of the alga were changed obviously, the absorption peak at 440 nm disappeared and there were two absorption peak around 430 nm and 450 nm, respectively. Ni2+ had no significant effect on the absorption spectrum of phycocyanin and allophycocyanin. When the Ni2+ concentration of 1.0 μg/mL, the alga still grew normally. It is concluded that 0.5 μg/mL trace Ni2+ is the best concentration to promote the growth and biomass accumulation of spirulina by increasing the content of phycobilin and changing the absorption spectrum of chlorophyll a in the blue and purple region. The finding provides the basis for reducing the production cost of Spirulina and controlling the nickel pollution of the water body by using the spirulina.
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2021, Vol. 40 
