Growth and Biochemical Composition of a Halotolerant Diatom Strain of Craticula sp.
ZHANG Qi, ZHENG Lingling, LI Tianli, LIU Jin, SONG Lirong
State Key Laboratory of Freshwater Ecology and Biotechnology, Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
Abstract：The naviculoid genus Craticula was widely distributed in alkaline saline waters. The halotolerant diatom strain Craticula sp. FACHB-2450 was isolated from saline lake in this work. The present study evaluated the effect of different salinities on growth, polysaccharides, soluble proteins, lipids and fatty acid profiles of strain FACHB-2450, as well as the photosynthetic physiological response under laboratory conditions. It was found that the maximal biomass concentration cultured was 1.79 g/L at 6% NaCl . The Fv/Fm value was increased up to 0.63 at 3% NaCl then decreased at higher salinity, with the maximal polysaccharides content cultured of 9.96% of dry weight at 6% NaCl. The soluble protein content was decreased from 22.78% to 23.65% of dry weight at 1.5%—9% NaCl sharply to 8.39% of dry weight at 12% NaCl. The maximal total lipid content cultured at 3% NaCl was 32.46% of dry weight. The main fatty acid components of strain FACHB-2450 were palmitoleic acid (C16:1) (30.17%—43.75% of total fatty acid), palmitic acid (C16:0) (22.53%—31.45% of total fatty acid), and eicosapentaenoic acid (EPA, C20:5) (14.79%—20.92% of total fatty acid). The content of the most abundant unsaturated fatty acid C20:5 was increased at lower salinity then decreased at higher salinity with the maximal concentration at 9% NaCl.
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