Histological Observation of Gonadal Development of Tiger Puffer Takifugu rubripes
LI Weiyuan1,2, XIONG Yuyu1,2, WANG Ziwei1,2, ZHANG Qi1,2, JIANG Jieming1,2, YAN Hongwei1,2, LIU Ying1,2,3, LIU Qi1,2, LI Meiyuan1, WANG Xiuli1
1. Dalian Ocean University, Dalian 116023, China; 2. Key Laboratory of Facility Fisheries, Ministry of Education, Dalian 116023, China; 3. Qingdao Pilot National Laboratory of Marine Science and Technology, Qingdao 266237, China
Abstract：In order to understand gonad development of tiger puffer Takifugu rubripes, 212 samples of tiger puffer in different developmental stages were collected from June 2019 to April 2021. It was found that the female of tiger puffer was a one-time spawning type, with closed-type testis and ovaries. According to the process of spermatogenesis and oogenesis, the testis of males was cystic type, and ovaries of females were partly synchronized. According to the morphological structure of oocytes, the accumulation of yolk matter and the changes in follicular cells, oogenesis was divided into 6 phases, and spermatogenesis divided into 5 phases according to the changes in spermatogenic morphology and structure. According to the external morphological characteristics, size, color, and histological characteristics of the gonads, the development process of the ovaries and testes were divided into six stages. In addition, the male and female gonadosomatic index (GSI) was shown to be increased rapidly after entered the developmental stage, with peak at stage Ⅴ, significantly higher than the pre-developmental level (P<0.05), and significantly decreased after spawning and ovulation (P<0.05), and gonadal development maintained a consistent trend of change. There was no significant difference in the hepatosomatic index (HSI) between stage Ⅱand stage Ⅲ during the development of the testis in the male (P>0.05), and then the HSI was gradually decreased. Female changes were different from male, without significant difference in HSI of female between samples at various periods (P>0.05). However, the condition factor (CF) of the male and female did not change significantly throughout the reproductive cycle (P>0.05). The findings are of great significance to the cognition of the reproduction biology of tiger puffer.
李伟缘, 熊玉宇, 王子维, 张琦, 姜洁明, 闫红伟, 刘鹰, 刘奇, 李美沅, 王秀利. 红鳍东方鲀性腺发育的组织学研究[J]. 水产科学, 2022, 41(5): 749-758.
LI Weiyuan, XIONG Yuyu, WANG Ziwei, ZHANG Qi, JIANG Jieming, YAN Hongwei, LIU Ying, LIU Qi, LI Meiyuan, WANG Xiuli. Histological Observation of Gonadal Development of Tiger Puffer Takifugu rubripes. Fisheries Science, 2022, 41(5): 749-758.
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2022, Vol. 41 
