Effects of Dietary Immunity Induced Pupae Powder of Antheraea pernyi on Growth, Non-Specific Immunity and Disease Resistance of Tiger Puffer Takifugu rubripes
SUN Yongxin1, MA Shuhui1, WANG Qingzhi1, REN Tongjun2, DU Xingfan1, LI Shuying1
1. Dalian Key Laboratory of Genetic Resources for Marine Shellfish, Liaoning Ocean andFisheries Science Research Institute, Dalian 116023, China; 2. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China
Abstract：Immunity induced pupae powder (IPP) of Antheraea pernyi was supplemented as feed additive to investigate its effects on the growth, immunity and disease resistance of the juvenile tiger buffer Takifugu rubripes with body weight of (58.52±2.07) g. There were five groups in triplication including basic feed group as the blank group, 0.5%, 1.0% and 2.0% dose of IPP were added to the basic feed as the test group, and 1.0% pupa powder was added to the basic feed as the control group. The supplementary feeding experimental lasted for 30 days. The results showed that the weight gain rate of the fish was significantly increased with the increase in the amount of IPP in the feed compared with the blank group (P<0.05). Furthermore, the fish fed diets containing 1.0% and 2.0% IPP had significantly higher weight gain rate than the fish in the blank group and the control group (P<0.05). The effects of IPP on the serum immune index were found different in various aspects did. The activities of acid phosphatase (ACP), superoxide dismutase (SOD) and catalase (CAT) in 0.5% IPP group, alkine phosphatase (AKP) and CAT in 1.0% IPP group, and CAT in 2.0% IPP group were significantly higher than those in blank group and control group (P<0.05). The immunoprotective effect was analysed using the pathogenicity challenge test and the cumulative mortality rate in the blank group was 100% after 7 days, and the immunoprotective rate was increased obviously, with the maximal immunoprotective rate of 50% in 0.5% IPP group. Above all, the supplement of 0.5% IPP in diet led to effectively increase weight gain rate, immunity and disease resistance, which can be used in fish production.
孙永欣, 马淑慧, 王庆志, 任同军, 都兴范, 李树英. 饲喂柞蚕免疫蛹粉对红鳍东方鲀生长、免疫和抗病力的影响[J]. 水产科学, 2022, 41(2): 236-242.
SUN Yongxin, MA Shuhui, WANG Qingzhi, REN Tongjun, DU Xingfan, LI Shuying. Effects of Dietary Immunity Induced Pupae Powder of Antheraea pernyi on Growth, Non-Specific Immunity and Disease Resistance of Tiger Puffer Takifugu rubripes. Fisheries Science, 2022, 41(2): 236-242.
[1] 王奎旗,陈梅,高天翔.东方鲀属鱼类的分类与区系分布研究[J].青岛海洋大学学报(自然科学版),2001,31(6):855-860. [2] 张涛,徐思祺,宋颖.红鳍东方鲀的病害防治简述[J].科学养鱼,2017(6):65-67. [3] 刘晓晖.洞庭湖流域水环境中典型抗生素污染特征、来源及风险评估[D].济南:山东师范大学,2017. [4] 都兴范.新型饲料蛋白质柞蚕免疫活性蛋白的研究应用[J].饲料研究,1997(11):2-3. [5] 孙永欣,温志新,米锐,等.柞蚕免疫蛹粉在刺参健康养殖中的应用研究[J].中国饲料,2016(15):19-23. [6] 孙永欣,马淑慧,米锐,等.柞蚕免疫蛹粉在凡纳滨对虾健康养殖中的应用研究[J].中国饲料,2017(15):20-23. [7] 吕贵仁,刘隽彦,陈丹.柞蚕抗菌素稳定性的研究[J].北方蚕业,2011,32(1):25-26. [8] 王宝贵,孙晓霞,赵林伊,等.柞蚕蛹蛋白质营养价值研究[J].中国公共卫生,2002,18(5):575-576. [9] 郭林英,王宇锋.柞蚕蛹替代鱼粉对鲤鱼生产性能影响研究[J].河南水产,2009(2):31-32. [10] 吉红,程小飞,李杰,等.蚕蛹替代鱼粉对框鳞镜鲤幼鱼生长性能、体成分及健康状况的影响[J].水产学报,2012,36(10):1599-1611. [11] KURBANOV A R, MILUSHEVA R Y, RASHIDOVA S S, et al. Effect of replacement of fish meal with silkworm (Bombyx mori) pupa protein on the growth of Clarias gariepinus fingerling [J]. International Journal of Fisheries and Aquatic Studies,2015,2(6):25-27. [12] MA S H, SUN Y X, WANG F Q, et al. Effects of tussah immunoreactive substances on growth, immunity, disease resistance against Vibrio splendidus and gut microbiota profile of Apostichopus japonicus[J].Fish & Shellfish Immunology,2017,63:471-479. [13] MI R, SUN Y X, LI J G, et al. Immune-related proteins detected through iTRAQ-based proteomics analysis of intestines from Apostichopus japonicus in response to tussah immunoreactive substances[J].Fish & Shellfish Immunology,2018,74:436-443. [14] RASMUSSEN R S. Quality of farmed salmonids with emphasis on proximate composition, yield and sensory characteristics[J].Aquaculture Research,2001,32(10):767-786. [15] GRIGORAKIS K. Compositional and organoleptic quality of farmed and wild gilthead sea bream (Sparus aurata) and sea bass (Dicentrarchus labrax) and factors affecting it:a review[J].Aquaculture,2007,272(1/2/3/4):55-75. [16] HEMRE G I, KARLSEN Ø, ECKHOFF K, et al. Effect of season, light regime and diet on muscle composition and selected quality parameters in farmed Atlantic cod, Gadus morhua L.[J].Aquaculture Research,2004,35(7):683-697. [17] JOHNSTON I A, LI X J, VIEIRA V L A, et al. Muscle and flesh quality traits in wild and farmed Atlantic salmon[J].Aquaculture,2006,256(1/2/3/4):323-336. [18] 陶宁萍,龚玺,刘源,等.三种养殖河豚鱼肌肉营养成分分析及评价[J].营养学报,2011,33(1):92-94. [19] 徐如卫,申屠基康,江锦坡,等.3种不同规格瓯江彩鲤含肉率及肌肉营养成分比较[J].宁波大学学报(理工版),2012,25(1):13-19. [20] 杜强,王艳艳,曾圣,等.三种不同规格杂交鲟含肉率及肌肉营养成分比较[J].中国饲料,2017(24):15-19. [21] 张永安,孙宝剑,聂品.鱼类免疫组织和细胞的研究概况[J].水生生物学报,2000,24(6):648-654. [22] 周进,宋晓玲,黄倢,等.A3α肽聚糖对牙鲆不同组织中超氧化物歧化酶及磷酸酶活性的影响[J].中国水产科学,2004,11(4):296-301. [23] CALLEWAERT L, MICHIELS C W. Lysozymes in the animal kingdom[J].Journal of Biosciences,2010,35(1):127-160. [24] FLETCHER T C. Modulation of nonspecific host defenses in fish[J].Veterinary Immunology and Immunopathology,1986,12(1/2/3/4):59-67. [25] 周婧,王旭,刘霞,等.饲料中牛磺酸水平对红鳍东方鲀免疫及消化酶的影响[J].大连海洋大学学报,2019,34(1):101-108. [26] 董文娟,王学英,张涛,等.柞蚕滞育蛹抗菌物质产生及其抑菌活性研究[J].安徽农业科学,2007,35(4):990-991.
2022, Vol. 41 
