Effects of Dietary Immunity Induced Pupae Powder of Antheraea pernyi on Growth, Non-Specific Immunity and Disease Resistance of Tiger Puffer Takifugu rubripes
SUN Yongxin1, MA Shuhui1, WANG Qingzhi1, REN Tongjun2, DU Xingfan1, LI Shuying1
1. Dalian Key Laboratory of Genetic Resources for Marine Shellfish, Liaoning Ocean andFisheries Science Research Institute, Dalian 116023, China; 2. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China
Abstract：Immunity induced pupae powder (IPP) of Antheraea pernyi was supplemented as feed additive to investigate its effects on the growth, immunity and disease resistance of the juvenile tiger buffer Takifugu rubripes with body weight of (58.52±2.07) g. There were five groups in triplication including basic feed group as the blank group, 0.5%, 1.0% and 2.0% dose of IPP were added to the basic feed as the test group, and 1.0% pupa powder was added to the basic feed as the control group. The supplementary feeding experimental lasted for 30 days. The results showed that the weight gain rate of the fish was significantly increased with the increase in the amount of IPP in the feed compared with the blank group (P<0.05). Furthermore, the fish fed diets containing 1.0% and 2.0% IPP had significantly higher weight gain rate than the fish in the blank group and the control group (P<0.05). The effects of IPP on the serum immune index were found different in various aspects did. The activities of acid phosphatase (ACP), superoxide dismutase (SOD) and catalase (CAT) in 0.5% IPP group, alkine phosphatase (AKP) and CAT in 1.0% IPP group, and CAT in 2.0% IPP group were significantly higher than those in blank group and control group (P<0.05). The immunoprotective effect was analysed using the pathogenicity challenge test and the cumulative mortality rate in the blank group was 100% after 7 days, and the immunoprotective rate was increased obviously, with the maximal immunoprotective rate of 50% in 0.5% IPP group. Above all, the supplement of 0.5% IPP in diet led to effectively increase weight gain rate, immunity and disease resistance, which can be used in fish production.
孙永欣, 马淑慧, 王庆志, 任同军, 都兴范, 李树英. 饲喂柞蚕免疫蛹粉对红鳍东方鲀生长、免疫和抗病力的影响[J]. 水产科学, 2022, 41(2): 236-242.
SUN Yongxin, MA Shuhui, WANG Qingzhi, REN Tongjun, DU Xingfan, LI Shuying. Effects of Dietary Immunity Induced Pupae Powder of Antheraea pernyi on Growth, Non-Specific Immunity and Disease Resistance of Tiger Puffer Takifugu rubripes. 水产科学, 2022, 41(2): 236-242.
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2022, Vol. 41 
