Cloning and Association Analysis with Growth Traits of igf Gene in Ussuri Catfish Pseudobagrus ussuriensis
WEI Mengya, WANG Simeng, NING Jing, FAN Fan, ZHU Chuankun
Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection, Jiangsu Engineering Laboratory for Breeding of Special Aquatic Organisms, School of Life Science, Jiangsu Key Laboratory for Eco-Agriculture Biotechnology Around Hongze Lake, Huaiyin Normal University, Huaian 223300, China
Abstract：In order to understand the relationship between insulin-like growth factor (igf) genes and growth traits, sequences of igf1 and igf2 genes were cloned for Ussuri catfish Pseudobagrus ussuriensis through referring to igf sequences of its close-related fishes, and relationships between polymorphic sites of igf and growth traits were analyzed by association analysis. The cDNA length of the igf1gene was 1241 bp, containing an open reading frame (ORF) of 477 bp coding a peptide with 158 amino acids (aa), and the DNA sequence of the igf1 gene with a length of 10 922 bp containing 5 exons and 4 introns was also obtained. The total cDNA length of the igf2 gene was 2008 bp with an ORF of 741 bp coding a peptide consisting 246 aa, and a 3700 bp DNA segment containing 5 exons and 4 introns was cloned. The aa sequences of the two peptides IGF1 and IGF2 of Ussuri catfish had the highest identities with those of yellow catfish Pelteobagrus fulvidraco (96.9% and 96.7%). A total of five simple sequence polymorphism sites (SNPs) were identified in the igf1 gene with 1 in exon and 4 in introns, and 7 microsatellites were also found. The number of identified SNPs in the igf2 gene was also 5 with 1 in exon and 4 in introns, and 5 microsatellites were identified. The 2 SNPs in exons of igf1 and igf2 genes were selected and their associations with 5 growth traits were also analyzed, but no significant associations were detected. Nevertheless, the finding in this study will provide useful references for further studies of igf genes in Ussuri catfish.
魏梦雅, 王思梦, 宁静, 范帆, 朱传坤. 乌苏里拟鲿igf基因克隆及其与生长性状关联分析[J]. 水产科学, 2022, 41(5): 738-748.
WEI Mengya, WANG Simeng, NING Jing, FAN Fan, ZHU Chuankun. Cloning and Association Analysis with Growth Traits of igf Gene in Ussuri Catfish Pseudobagrus ussuriensis. Fisheries Science, 2022, 41(5): 738-748.
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2022, Vol. 41 
