Differences in Carapace Geometric Morphometrics of Eriocheir sinensis from Various Geographical Origins in the Changjiang River System
TANG Jing1, XUE Junren1, LIU Hongbo2, JIANG Tao2, CHEN Xiubao2, YANG Jian1,2
1. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; 2. Laboratory of Fishery Microchemistry, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
Abstract：In order to effectively identify the morphological differences of Chinese mitten crab Eriocheir sinensis in the middle and lower reaches of the Changjiang River system, geometric morphometric analysis was conducted to comparatively understand the carapace morphological characteristics of the crab from seven different well-known geographical origins, i.e., the Datong Lake in Hunan Province. the Huangpi Lake, Tuo Lake, Nvshan Lake, and Wuwei section of the Changjiang River in Anhui Province, as well as the Qin Lake and Shijiu Lake in Jiangsu Province. After computer software-mediated image processing, 35-landmarks were extracted and discriminant analysis was carried out. The morphological variation vector was visualized by thin plate spline analysis and mesh deformation. The results revealed that the carapace morphological characteristicsof the crab from the aforementioned origins differed from each other. In comparison with the average carapace shape, although no significant variation was found in that of Wuwei wild population, significant differences were documented in those from the other six origins, i.e., with longer frontal and lateral teeth, smaller M pattern, and smaller posterior edge of carapace for those from the three lake origins of Anhui Province.For the crab from the remaining three lake origins, the frontal and lateral teeth were shorter, the M pattern was larger, and the rear margin area of the posterior edge of carapace was wider. Moreover, the identification accuracies were as high as 100% according to geometric morphometric landmarks of both female and male crab among different origins. Our study demonstrated that geometric morphometric analysis could provide a convenient, fast, nonlethal and innovative approach for geographical origin traceability at thefine-scale and protection of E. sinensis.
唐静, 薛竣仁, 刘洪波, 姜涛, 陈修报, 杨健. 长江水系中华绒螯蟹背甲几何形态的产地差异[J]. 水产科学, 2023, 42(5): 775-784.
TANG Jing, XUE Junren, LIU Hongbo, JIANG Tao, CHEN Xiubao, YANG Jian. Differences in Carapace Geometric Morphometrics of Eriocheir sinensis from Various Geographical Origins in the Changjiang River System. 水产科学, 2023, 42(5): 775-784.
[1] 堵南山.关于绒螯蟹属的分类[J].水产科技情报,2002,29(1):10-12. [2] 郑海波.中华绒螯蟹的品质分析与比较[D].无锡:江南大学,2008. [3] 刘锁明,张增,杨建新,等.长荡湖野生中华绒螯蟹肌肉营养成分的分析与评价[J].水产养殖,2021,42(7):23-28. [4] 谢辉,尹明雨,张玉非,等.不同产地雌性中华绒螯蟹感官品质与滋味品质的差异性[J].食品与发酵工业,2021,47(6):114-120. [5] ROBINSON B W,WILSON D S.Genetic variation and phenotypic plasticity in a trophically polymorphic population of pumpkinseed sunfish (Lepomis gibbosus)[J].Evolutionary Ecology,1996,10(6):631-652. [6] 郑朝臣,姜涛,骆仁军,等.不同产地中华绒螯蟹形态差异的地标点法分析[J].水产学报,2017,41(12):1896-1907. [7] 薛竣仁,姜涛,陈修报,等.阳澄湖原产及 “洗澡” 中华绒螯蟹背甲的几何形态分析[J].水生生物学报,2020,44(3):587-594. [8] 刘翚.特色农产品原产地养殖现状与发展研究——以阳澄湖大闸蟹为例[J].中国农机化学报,2014,35(5):183-186. [9] 张列士,姜治忠,李军.日本绒螯蟹与不同水系中华绒螯蟹的形态比较[J].上海水产大学学报,2002,11(2):110-113. [10] COOKE S B,TERHUNE C E.Form,function,and geometric morphometrics[J].Anatomical Record,2015,298(1):5-28. [11] BOOKSTEIN F L.Morphometric Tools for Landmark Data: Geometry and Biology[M].Cambridge: Cambridge University Press,1998:55-87. [12] LUO R J,JIANG T,CHEN X B,et al.Determination of geographic origin of Chinese mitten crab (Eriocheir sinensis) using integrated stable isotope and multi-element analyses[J].Food Chemistry,2019,274:1-7. [13] 赵鉴,苏彦平,刘洪波,等.不同/相同水系中华绒螯蟹元素“指纹”的产地差异性[J].核农学报,2014,28(7):1253-1260. [14] 杨文斌,苏彦平,刘洪波,等.长江水系3个湖泊中华绒螯蟹形态及元素“指纹”特征[J].中国水产科学,2012,19(1):84-93. [15] 姜晓东,成永旭,潘建林,等.基于地标点几何形态测量法区分不同水系野生中华绒螯蟹[J].中国水产科学,2019,26(6):1116-1125. [16] 姜晓东,成永旭,潘建林,等.中国长江与荷兰野生中华绒螯蟹的头胸甲形态特征比较[J].淡水渔业,2020,50(1):38-43. [17] 姜晓东,成永旭,杨雨虹,等.基于地标点法的绥芬河绒螯蟹种群鉴定[J].水产科学,2021,40(4):501-507. [18] ZHENG C C,JIANG T,LUO R J,et al.Geometric morphometric analysis of the Chinese mitten crab Eriocheir sinensis:a potential approach for geographical origin authentication[J].North American Journal of Fisheries Management,2021,41(4):891-903. [19] XUE J R,JIANG T,CHEN X B,et al.Multi-mineral element profiles in genuine and “bathing” cultured Chinese mitten crabs (Eriocheir sinensis) in Yangcheng Lake,China[J].Fishes,2022,7(1):11. [20] ROHLF F J.tpsDig2[EB/OL].(2021-01-24)[2021-12-17]. https://www.sbmorphometrics.org/. [21] ROHLF F J.tpsRelw[EB/OL].(2021-01-24)[2021-12-17]. https://www.sbmorphometrics.org/. [22] ROHLF F J.Relative warp analysis and an example of its application to mosquito wings[J].Contributions to Morphometrics,1993:131-159. [23] ROHLF F J.tpsRegr[EB/OL].(2021-01-24)[2021-12-17].http://sbmorphometrics.org/. [24] 陈新军,方舟,苏杭,等.几何形态测量学在水生动物中的应用及其进展[J].水产学报,2013,37(12):1873-1885. [25] BOOKSTEIN F L.Principal warps:thin-plate splines and the decomposition of deformations[J].IEEE Transactions on Pattern Analysis and Machine Intelligence,1989,11(6):567-585. [26] 王梦瑶,孙伟琪,陈冰冰,等.贝叶斯判别分析在财务预警中的应用[J].品牌与标准化,2021(1):59-61. [27] 黄朝辉,母昌考,胡丹丹,等.三疣梭子蟹蜕壳周期肝胰腺、外壳和鳃中钙含量的变化[J].生物学杂志,2012,29(5):22-24. [28] GREENAWAY P.Calcium balance and moulting in the Crustacea[J].Biological Reviews,1985,60(3):425-454. [29] 杜正彩,邓家刚,王志萍,等.蟹壳化学成分与药理作用研究进展[J].安徽农业科学,2011,39(17):10503-10505. [30] RUFINO M,ABELLÓ P,YULE A B.Male and female carapace shape differences in Liocarcinus depurator (Decapoda,Brachyura):an application of geometric morphometric analysis to crustaceans[J].Italian Journal of Zoology,2004,71(1):79-83. [31] SÁNCHEZ M,GOROSPE J,GOROSPE J,et al.Describing geographic differences in carapace shape in the blue swimming crab Portunus pelagicus from Mindanao Bays,Philippines[J].AACL Bioflux,2013,6(6):622-634. [32] GRINANG J,DAS I,NG P K L.Geometric morphometric analysis in female freshwater crabs of Sarawak (Borneo) permits addressing taxonomy-related problems[J].PeerJ,2019,7:e6205. [33] 苏杭.几何形态测量学法在大洋性头足类种间及种群鉴别中的应用[D].上海:上海海洋大学,2016. [34] PONTON D.Is geometric morphometrics efficient for comparing otolith shape of different fish species?[J].Journal of Morphology,2006,267(6):750-757. [35] 彭凯秀,崔艳梅,姜芳,等.不同产地三疣梭子蟹的稳定同位素比值特征及原产地溯源[J].应用生态学报,2021,32(6):2021-2027. [36] 张辉.由阳澄湖大闸蟹被假冒看原产地保护困境[J].食品界,2018(11):78-79.
2023, Vol. 42 
