Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China
Abstract：In order to explore the effects of preservation conditions on the waterless preservation time of hybrid grouper Epinephelus fuscoguttatus ♀×E. lanceolatus ♂, a single factor test was carried out to screen the optimal range of conditions by using the proportion of O2, CO2 and N2 gases, survival temperature and moisturizing materials, and orthogonal experiment was conducted to optimize the preservation conditions of atmosphere preservation-alive. The muscle components and blood biochemical indices in different survival stages were determined. The results showed that the maximal waterless survival period was observed under the gas ratio of V(O2)∶V(CO2)∶V(N2)=85∶0∶15, the preservation temperature of 15 ℃, and the moisturizing material of sponge, with the 12.5 h survival rate of 100%. The weight loss rate of hybrid grouper was increased gradually with the prolongation of survival time, and the cumulative weight loss rate reached (6.49±0.15)% after rehydration for 12 h. There was no significant change in moisture content in dorsal muscle(P＞0.05), but the ash content was slightly increased. The contents of crude protein and crude fat were gradually decreased, and the decrease of fat content during the whole process was much greater than that of protein, which might be because the main energy supply substance in the body of the hybrid grouper was fat rather than protein during the period of atmosphere preservation-alive and maintenance. The blood glucose and cortisol contents showed curve changes with the prolonging of survival time. The contents of BUN, AST, ALT and ACP were significantly increased, while the contents of TG and AKP were decreased. In conclusion, the kidney, liver and immune system of the hybrid grouper were all damaged to a certain extent during atmosphere preservation-alive, but they all recovered after resuscitation, and the survival time was longer than that in pure oxygen environment. Therefore, the optimal atmosphere preservation-alive conditions selected were conducive to the waterless preservation transportation of grouper. The finding will provide theoretical support with the practical application of atmosphere preservation-alive and preservation transportation of grouper.
[1] ALGERS B, ANIL H, BLOKHUIS H, et al. Project to develop animal welfare risk assessment guidelines on stunning and killing[J].EFSA Supporting Publications,2009,6(7):11E. [2] 农业农村部渔业渔政管理局,全国水产技术推广总站,中国水产学会.中国渔业统计年鉴2020[M].北京:中国农业出版社,2020. [3] 范秀萍.珍珠龙胆石斑鱼低温休眠无水保活的胁迫响应与机制研究[D].湛江:广东海洋大学,2019. [4] 范秀萍,秦小明,章超桦,等.珍珠龙胆石斑鱼肌肉营养成分与挥发性风味成分的分析与评价[J].广东海洋大学学报,2018,38(1):39-46. [5] 朱乾峰,陈鹏文,范秀萍,等.珍珠龙胆石斑鱼低温有水保活条件优化[J].食品工业科技,2018,39(22):276-282. [6] 吴波,谢晶.石斑鱼有水活运工艺中温度、盐度的优化[J].食品科学,2019,40(16):235-241. [7] 朱乾峰.珍珠龙胆石斑鱼低温保活运输技术研究[D].湛江:广东海洋大学,2018. [8] MASTROMATTEO M, CONTE A, DEL NOBILE M A. Combined use of modified atmosphere packaging and natural compounds for food preservation[J].Food Engineering Reviews,2010,2(1):28-38. [9] 王红,王少华,熊光权,等.水产品保鲜技术研究及发展趋势[J].湖北农业科学,2019,58(12):15-18. [10] 汤元睿,谢晶.金枪鱼气调保鲜技术的研究进展[J].食品科学,2014,35(9):296-300. [11] 田标,陈申如,杨远帆,等.黑鲷无水保活技术的初步研究[J].集美大学学报(自然科学版),2004,9(3):221-225. [12] 李丹.滩涂贝类净化及气调包装保活保鲜技术研究[D].杭州:浙江工商大学,2008. [13] 韩利英,张慜.鲫鱼保活条件对存活率的影响[J].食品与生物技术学报,2009,28(5):642-646. [14] 申淑琦,万玉美,申亮,等.温度、湿度和氧气对海湾扇贝无水保活的影响[J].大连海洋大学学报,2014,29(5):492-497. [15] 白艳龙.黄颡鱼保活技术研究[D].上海:上海海洋大学,2013. [16] NIE X B, ZHANG F, WANG T T, et al. Physiological and morphological changes in turbot (Psetta maxima) gill tissue during waterless storage[J].Aquaculture,2019,508:30-35. [17] 徐德峰,王雅玲,孙力军,等.降温及生态冰温条件对凡纳滨对虾无水存活时间的影响[J].广东海洋大学学报,2019,39(6):101-107. [18] 张玉晗,谢晶.包装充氧量对无水活运花鲈鳃组织结构及相关酶活性的影响[J].食品科学,2020,41(15):269-274. [19] 刘慧慧,刘俊荣,田元勇,等.菲律宾蛤仔的干湿藏保活特性初探[J].水产科学,2017,36(3):267-273. [20] 徐中平.星斑川鲽无水保活运输技术研究[J].科技创新导报,2017,14(4):75-76. [21] SALIN K R. Live transportation of Macrobrachium rosenbergii (de man) in chilled sawdust[J].Aquaculture Research,2005,36(3):300-310. [22] 徐子涵,茅林春.虾保活运输的关键技术及装备研究进展[J].食品工业科技,2018,39(9):306-310. [23] FAN X P, QIN X M, ZHANG C H, et al. Metabolic and anti-oxidative stress responses to low temperatures during the waterless preservation of the hybrid grouper (Epinephelus fuscogutatus♀×Epinephelus lanceolatus ♂)[J].Aquaculture,2019,508:10-18. [24] 李卢,张长峰,吴佳静,等.高压对鲫鱼无水保活的影响[J].食品工业科技,2020,41(20):261-265. [25] 刘骁,谢晶,杨茜,等.无水保活条件下团头鲂生理应激及鱼肉品质的变化[J].农业工程学报,2016,32(3):295-300. [26] 范秀萍,秦小明,章超桦,等.温度对有水保活石斑鱼代谢与鱼肉品质的影响[J].农业工程学报,2018,34(14):241-248. [27] 张晓林,王秋荣,刘贤德.鱼类保活运输技术研究现状及展望[J].渔业现代化,2017,44(1):40-44. [28] 吴佳静,杨悦,许启军,等.水产品保活运输技术研究进展[J].农产品加工,2016(16):55-56. [29] 王际英,张德瑞,马晶晶,等.珍珠龙胆石斑鱼肌肉营养成分分析与品质评价[J].海洋湖沼通报,2015(4):61-69. [30] IBARZ A, PADRÓS F, GALLARDO M Á, et al. Low-temperature challenges to gilthead sea bream culture:review of cold-induced alterations and ‘Winter Syndrome'[J].Reviews in Fish Biology and Fisheries,2010,20(4):539-556. [31] 许友卿,郑一民,丁兆坤.冷应激对水生动物代谢的影响及调控研究进展[J].中国水产科学,2017,24(5):1149-1159. [32] 杜浩,危起伟,甘芳,等.美洲鲥应激后皮质醇激素和血液生化指标的变化[J].动物学杂志,2006,41(3):80-84. [33] GUO J S, WU P H, CAO J L, et al. The PFOS disturbed immunomodulatory functions via nuclear factor-κB signaling in liver of zebrafish (Danio rerio)[J].Fish & Shellfish Immunology,2019,91:87-98. [34] 明建华,谢骏,徐跑,等.大黄素、维生素C及其配伍对团头鲂生长、生理生化指标、抗病原感染以及两种HSP70s mRNA表达的影响[J].水产学报,2010,34(9):1447-1459. [35] 蒋春琴,冷向军,李小勤,等.醋酸棉酚对异育银鲫生长性能、血清生化指标和棉酚残留的影响[J].动物营养学报,2012,24(1):168-175. [36] 吴胜泽,熊波,黄钟标,等.无水保活下鲫鱼生理生化指标的变化[J].食品安全质量检测学报,2018,9(15):3992-3998. [37] 王维政,曾泽乾,黄建盛,等.低氧胁迫对军曹鱼幼鱼抗氧化、免疫能力及能量代谢的影响[J].广东海洋大学学报,2020,40(5):12-18.
2023, Vol. 42 
