Stress Relieving Effect of Taurine and Glutamine on Grouper Hybrid
MI Hongbo1, LU Yue1, CHEN Jingxin1, LI Xuepeng1, LI Jianrong1,2
1. National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, National Research & Development Branch Center of Surimi and Surimi Products Processing, College of Food Science and Technology, Bohai University, Jinzhou 121013, China; 2. Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China
Abstract：In order to relieve the stress effect of hybrid grouper (Epinephelus fuscoguttatus ♀×E. lanceolatus ♂) during low-temperature and air exposure alive, the grouper with body weight of (800±50) g were temporarily retained in seawater containing 0 (control group) and 20 mg/L taurine (taurine group), and then 0 (control group), 20, 25 and 30 mg/L glutamine. The plasma biochemical indices, liver metabolites, antioxidant enzyme activities and muscle metabolism of the grouper in fresh state, low-temperature and air exposure alive for 0, 6, 12 h, and after revival were analyzed. The results showed that there was significant decrease in the concentration of cortisol in the grouper exposed to addition of taurine and glutamine in the temporary water, leading to enable to quickly adapt the low temperature environments. The liver metabolism was disturbed during air exposure alive, with enhanced activities of catalase, total superoxide dismutase, and glutathione peroxidase and total antioxidant capacity in liver. The contents of glucose, urea nitrogen and cortisol, and the activities of aspartate aminotransferase and alanine aminotransferase were found to be increased steadily with the prolongation of air exposure, with the peak value at 6 or 12 h. The significant decrease in content of muscle glycogen was observed in each group, and the significant increase in content of lactic acid and gradual recovery after revival. The changes in related indicators of the grouper treated with taurine and glutamine solution, especially taurine and 30 mg/L glutamine group, were shown to be significantly lower than those in control group and taurine treatment group, indicating that the addition of taurine and glutamine into water led to the hybrid grouper to maintain the stability of physiological and biochemical indicators and effectively alleviate the degree of oxidative stress damage during air exposure.
米红波, 鹿悦, 陈敬鑫, 李学鹏, 励建荣. 牛磺酸和谷氨酰胺对石斑鱼的应激缓解作用[J]. 水产科学, 2024, 43(6): 865-874.
MI Hongbo, LU Yue, CHEN Jingxin, LI Xuepeng, LI Jianrong. Stress Relieving Effect of Taurine and Glutamine on Grouper Hybrid. Fisheries Science, 2024, 43(6): 865-874.
[1] XAVIER B, MEGARAJAN S, RANJAN R, et al. Effect of packing density on selected tissue biochemical parameters of hatchery produced fingerlings of orange spotted grouper Epinephelus coioides (Hamilton, 1822) during transportaion[J]. Indian Journal of Fisheries,2018,65(2):138-143. [2] RIMMER M A, GLAMUZINA B. A review of grouper (Family Serranidae:Subfamily Epinephelinae) aquaculture from a sustainability science perspective[J]. Reviews in Aquaculture,2019,11(1):58-87. [3] 徐子涵.南美白对虾的无水低温胁迫响应和无水保活运输装置[D].杭州:浙江大学,2018. [4] 袁仲瑾,岑剑伟,李来好,等.低温暂养对珍珠龙胆石斑鱼存活、非特异性免疫及抗氧化指标的影响[J].南方水产科学,2022,18(6):118-126. [5] EL-SAYED A F M. Is dietary taurine supplementation beneficial for farmed fish and shrimp?a comprehensive review[J]. Reviews in Aquaculture, 2014,6(4):241-255. [6] ABDEL-TAWWAB M, MONIER M N. Stimulatory effect of dietary taurine on growth performance, digestive enzymes activity, antioxidant capacity, and tolerance of common carp, Cyprinus carpio L., fry to salinity stress[J]. Fish Physiology and Biochemistry,2018,44(2):639-649. [7] MARTINS N, DIÓGENES A F, MAGALHÃES R, et al. Dietary taurine supplementation affects lipid metabolism and improves the oxidative status of European seabass (Dicentrarchus labrax) juveniles[J]. Aquaculture,2021,531:735820. [8] 虞为,杨育凯,林黑着,等.牛磺酸对花鲈生长性能、消化酶活性、抗氧化能力及免疫指标的影响[J].南方水产科学,2021,17(2):78-86. [9] KIM M H, KIM H. The roles of glutamine in the intestine and its implication in intestinal diseases[J]. International Journal of Molecular Sciences,2017,18(5):1051. [10] LI H T, FENG L, JIANG W D, et al. Oxidative stress parameters and anti-apoptotic response to hydroxyl radicals in fish erythrocytes:protective effects of glutamine, alanine, citrulline and proline[J]. Aquatic Toxicology,2013,126:169-179. [11] 周传朋,刘波,谢骏,等.抗应激制剂对运输团头鲂血清生化和肝脏抗氧化指标的影响[J].水产科学,2014,33(1):8-14. [12] 李豪华.斑点叉尾鮰运输应激缓解技术研究[D].兰州:兰州理工大学,2023. [13] 张玉晗.花鲈生态冰温无水活运技术的研究[D].上海:上海海洋大学,2019. [14] 王晓雯,朱华,马国庆,等.高溶氧对西伯利亚鲟幼鱼非特异性免疫指标的影响[J].四川农业大学学报,2017,35(1):93-98. [15] 王香丽,麦康森,徐玮,等.蛋氨酸对瓦氏黄颡鱼幼鱼肝脏及血浆中谷草转氨酶和谷丙转氨酶活力的影响[J].中国海洋大学学报(自然科学版),2015,45(9):49-53. [16] BAI C, XIONG G Q, XU P, et al. Effect of cold-anesthetization rate on blood biochemical parameters and muscle composition during live channel catfish Ictalurus punctatus waterless preservation[J]. Fisheries Science,2020,86(6):1043-1053. [17] 刘汝杰,李明晔,孙泽威,等.运输前灌服谷氨酰胺对运输应激雏鸡肠黏膜上皮细胞线粒体功能及坏死性凋亡信号通路的影响[J].动物营养学报,2023,35(2):1270-1278. [18] 朱乾峰.珍珠龙胆石斑鱼低温保活运输技术研究[D].湛江:广东海洋大学,2018. [19] KIM Y S, JUNG M H, CHOI M Y, et al. Glutamine attenuates tubular cell apoptosis in acute kidney injury via inhibition of the c-Jun N-terminal kinase phosphorylation of 14-3-3[J]. Critical Care Medicine,2009,37(6):2033-2044. [20] 张云龙,贺亚蒙,袁娟,等.运输过程中水质和鱼类生理指标的变化及运输控制策略[J].水生生物学报,2018,42(2):439-450. [21] NIE X B, ZHANG F, WANG T T, et al. Physiological and morphological changes in turbot (Psetta maxima) gill tissue during waterless storage[J]. Aquaculture,2019,508:30-35. [22] 谭南峰,王佳琪,袁鹏翔,等.CO2麻醉预处理对生态冰温保活运输大黄鱼的影响[J].食品科技,2022,47(8):96-104. [23] 张坤.金鲳鱼低温有水保活技术研究[D].湛江:广东海洋大学,2022. [24] KUGINO K, TAMARU S, HISATOMI Y, et al. Long-duration carbon dioxide anesthesia of fish using ultra fine (nano-scale) bubbles[J]. PLoS One,2016,11(4):e0153542. [25] 刘经纬,麦康森,徐玮,等.谷氨酰胺对半滑舌鳎稚鱼非特异性免疫相关酶活力和低氧应激后HIF-1α表达的影响[J].水生生物学报,2016,40(4):736-743. [26] SHI G P, GAO T Q, LI X H, et al. Integrating transcriptomic and metabolomic analysis to understand muscle qualities of red swamp crayfish (Procambarus clarkii) under transport stress[J]. Food Research International,2023,164:112361. [27] 陈付菊,付生云,令小东,等.低氧胁迫对青海湖裸鲤肌肉线粒体呼吸链复合体酶及抗氧化酶活性的影响[J].广东海洋大学学报,2021,41(6):118-124. [28] MEZZOMO N J, FONTANA B D, MÜLLER T E, et al. Taurine modulates the stress response in zebrafish[J]. Hormones and Behavior,2019,109:44-52. [29] SCHULLER-LEVIS G B, PARK E. Taurine and its chloramine:modulators of immunity[J]. Neurochemical Research,2004,29(1):117-126. [30] LI H T, JIANG W D, LIU Y, et al. The metabolites of glutamine prevent hydroxyl radical-induced apoptosis through inhibiting mitochondria and calcium ion involved pathways in fish erythrocytes[J]. Free Radical Biology and Medicine,2016,92:126-140. [31] LIU H L, YANG S P, WANG C G, et al. Effect of air exposure and resubmersion on the behavior and oxidative stress of Pacific white shrimp Litopenaeus vannamei[J]. North American Journal of Aquaculture,2015,77(1):43-49. [32] CHENG C H, MA H L, DENG Y Q, et al. Immune and physiological responses of mud crab (Scylla paramamosain) under air exposure[J]. Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology,2020,233:108767. [33] 温震威,黄建盛,陈有铭,等.L-丙氨酰-L-谷氨酰胺对低氧胁迫后军曹鱼幼鱼生长性能、血清生化与抗氧化指标的影响[J].动物营养学报,2023,35(4):2503-2513. [34] WANG Q, GE Q Q, CHEN Z, et al. The effect of air exposure and re-water on gill microstructure and molecular regulation of Pacific white shrimp Penaeus vannamei[J]. Fish & Shellfish Immunology,2023,132:108458. [35] 陈旭,左涛,周胜杰,等.饲喂甘草后低盐胁迫对尖吻鲈相关酶活性的影响[J].水产科学,2023,42(3):404-412. [36] SUN J L, ZHAO L L, LIAO L, et al. Interactive effect of thermal and hypoxia on largemouth bass (Micropterus salmoides) gill and liver:aggravation of oxidative stress,inhibition of immunity and promotion of cell apoptosis[J]. Fish & Shellfish Immunology,2020,98:923-936. [37] KIM W, KIM H U, LEE H N, et al. Taurine chloramine stimulates efferocytosis through upregulation of Nrf2-mediated heme oxygenase-1 expression in murine macrophages:possible involvement of carbon monoxide[J]. Antioxidants & Redox Signaling,2015,23(2):163-177. [38] 李佳琦.谷氨酰胺改善高温环境肉鸡骨骼肌和血清抗氧化性能的应用研究[D].滁州:安徽科技学院,2020. [39] 杜平.谷氨酰胺对鸡免疫功能调节作用的研究进展[J].中国饲料,2022(18):14-17. [40] 张玉晗,谢晶.保护液结合暂养工艺对花鲈无水活运效果的影响[J].食品科学,2019,40(23):262-268.
2024, Vol. 43 
