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月刊，1984年创刊
主管：内蒙古自治区教育厅
主办：包头医学院
主编：赵云山
特邀主编：高长青
编辑出版：包头医学院学报编辑部
国际刊号：ISSN 1006－740X
国内刊号：CN 15－1182/R
邮发代号：16-292

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2015 Vol. , No. 6 Published: 2015-06-25

Article

	335	Changes in Fishery Production and Structure in Lakeside Region of Poyang Lake
		ZHAO Dandan,LIU Junguo
		In this study ,the structure characteristics of fishery production were analyzed in lakeside region of Poyang Lake from 1996 to 2008 by sector Kuzenets K value and Moore structure value .The results showed that fish gross production was increased steadily by 6% ,of which culture was main section ,and catching production was nearly steady with a little decrease . There was obviously difference in fish production between different counties in lakeside region under the natural conditions .The fish production in Nanchang county was leading among all the counties , accounting for over 1/6 of the gross fish production in lakeside region due to exceptionally natural resources .In the w hole period ,the adjustment of fishery resource structure speeded up in speed and rate ,higher than Jiangxi provinces at the same period . There was significant difference in speed and rate between different counties in lakeside region .Before 2000 ,Xinjian County had the maximal K value (6 .2) and e value (4 .1°) .After 2001 ,however ,the maximal K value and e value were observed in Poyang County ,indicating that difference in the studied counties reflected the special trait on fishery policy and natural condition .To keep the sustainability of fishery resources in Poyang Lake ,fisheries and agricultural department should speed up to adjust the sector structure additionally ,to improve the area of fish farming and rice‐fishing and to decrease the use of artificial diet and organic fertilizer in the lake . We can create health water condition for fish to make economic profit and ecological benefit in Poyang Lake .
		2015 Vol. (6): 335-343 [Abstract] ( 86 ) HTML (0 KB) PDF (2513 KB) ( 427 )

	344	Canonical Correspondence between Phytoplankton Community and Environmental Factors in the Mainstream of Haihe River
		ZHANG Ping,LI Baohua,LIU Xianbin,BAI Ming,WANG Juanjuan,WANG Xiuqin,WANG Dexing,SHI Wenbo
		The phytoplankton community in mainstream of Haihe River was investigated in late spring , mid‐summer and early autumn from 2009 to 2013 ,and 8 phyla ,92 genera and 181 species of phytoplankton were detected in the waters .The phytoplankton was found to be predomninantly composed of Cyanophyta , Bacillariophyta and Chlorophyta ,including Scenedesmus quadricauda ,Chlorella vulgaris ,Oocystis sp ., Selenastrum sp .,and Caeteria sp in Chlorophyta ;Microcystis sp ., Phormidium tenue , Spirulina sp ., Oscillatoria sp .,and Dactylococcopsis sp .in Cyanophyta;Cyclotella sp .,Nitzschia sp .,Navicula sp .in Bacillariophyta and Cryptomonas erosa ,C .ovata in Cryptophyta .The relationships between the distribution of phytoplankton and environmental factors in each sampling site were studied by canonical correspondence analysis .The 5 year observation demonstrated that total nitrogen ,total phosphorus and pH were the key factors for the distribution of phytoplankton communities .
		2015 Vol. (6): 344-350 [Abstract] ( 59 ) HTML (0 KB) PDF (1718 KB) ( 250 )

	351	Diversity and Antimicrobial Activity of Lactic Acid Bacteria Isolated from Aquaculture
		LIU Jun,LIN Junfang,GUO Liqiong,YE Zhiwei,FANG Zaiguang,GUO Xinyue,LI Ziliang
		The experiment was conducted to study lactic acid bacteria distribution in different aquatic prod‐ucts and to screen lactic acid bacteria inhibiting common aquatic pathogenic bacteria .Lactic acid bacteria screening culture medium was applied to separate lactic acid bacteria and 16 s rDNA was used identify the lactic acid bacteria from guts of aquatic animals .The punching method bacteriostatic test was applied to test antibacterial activity of 15 strains of common aquatic pathogenic bacteria including lactic acid bacteria inhibiting Aeromonas hydrophila ,Vibrio parahaemolyticus ,V .alginolyticus and so on .In this study , 156 strains of lactic acid bacteria were isolated from the guts of 30 samples including fishes ,shrimps , shellfishes ,and clams from tropical waters in South Asia .Subsequently ,the species distribution and anti‐microbial activity of these isolates were investigated .Gram staining and microscopic inspection revealed that the morphology of these isolates were diversiform ,such as rod ,short rod ,sphere ,and chains .16S rDNA identification showed that these isolates were comprised of 11 species of lactic acid bacteria ,predom‐inant Lactococcus lactis ,Pediococcus pentosaceus ,and L .garvieae .According to the results ,Leuconostoc pseudomesenteroides ,L .mesenteroides ,P .pentosaceus ,L .lactis ,Lactobacillus sake ,and Weissella con‐f usa had a wide antimicrobial spectrum and efficiently antibacterial activity .The findings provide a theo‐retical basis for the application of lactic acid bacteria in aquaculture .
		2015 Vol. (6): 351-357 [Abstract] ( 112 ) HTML (0 KB) PDF (1036 KB) ( 570 )

	358	Effects of Density and Size of Gray Mullet Mugil cephalus on Survival and Growth Performance of Pacific White Leg Shrimp Litopenaeus vannamei in Polyculture
		PENG Yinhui,LIU Xujia,WANG Zhicheng,LIANG Zhihui,HUANG Guoqiang
		Gray mullet Mugil cephalus juveniles were stocked into 27 tanks at a rate of 0 ,0 .13 ,0 .26 ,0 .52 , 1 .04 ,and 2 .07 ind ./m2 in large gray mullet and 0 .13 ,0 .26 ,and 0 .52 ind/m2 in small gray mullet and poly‐cultured with Pacific white leg shrimp Litopenaeus vannamei at a rate of 100 ind ./m2 to study the effects of density and size of gray mullet on survival and grow th performance of Pacific w hite leg shrimp in poly‐culture .Results showed that there was no significant difference in the yield ,size and survival of the shrimp among different groups (P>0 .05) .The yield and size of shrimp were found to be decreased with the in‐crease in density of the fish in polyculture ,lower size and yield of the shrimp in the maximal fish density groups (2 .07 fish/m2 and 0 .52 fish/m2 ) than that in other groups .There was higher survival of shrimp in polyculture groups than that in the monoculture group ,the maximal survival in the maximal fish density groups .The total yield of shrimp and fish was significantly higher in the maximal fish density groups than that in other groups ,without significant difference in total yield among other polyculture groups ( P>0 .05) .The food conversion ratio was found to be lower in polyculture groups than that in the control group ,the minimum in the highest fish density (2 .07 fish/m2 ) group ,indicating that the food utilization ef‐ficiency was increased with the increase in fish density ,without significant differences among different groups (P>0 .05) .No significant differences in water temperature ,salinity ,dissolved oxygen level ,pH ,and BOD5 were observed among different groups during the experimental period (P>0 .05) ,even though the contents of ammonia‐N ,nitrite‐N ,and nitrate‐N were higher level in middle and later experiment than early stage (P> 0 .05) ,and the content of inorganic phosphorus was maintained at constant level during the whole experimental period .The findings indicated that polyculture of gray mullet at suitable size and densi‐ty did not lead to decrease in yield of Pacific white leg shrimp ,instead of increase in survival of shrimp and food utilization efficiency ,and improvement of water quality .
		2015 Vol. (6): 358-363 [Abstract] ( 75 ) HTML (0 KB) PDF (639 KB) ( 337 )

	364	Concentration and Proportion of Calcium and Magnesium in Water in Golden Shrimp Neocaridina sp Culture
		SONG Bo,CAO Shanmao,SUN Kai
		Influences of concentration and proportion of calcium and magnesium in water on growth and vi‐tality of golden shrimp (Neocaridinasp) at water temperature of 20-23 ℃ and pH 6 .5—7 .5 .The golden shrimp was reared in 20 cm × 15 cm × 20 cm glass cylinder at 20 shrimp per cylinder in the water containing total amount of calcium and magnesium at 35 mg/L ,70mg/L or 105 mg/L ,with ratio of calcium to mag‐nesium=1∶1 ,1∶2 ,1∶3 and 1∶4 for 20 days .The activities of alkaline phosphatase (AKP) ,alanine aminotransferase (GTP) ,aspartate aminotransferase (GOT) and Na+ /K+‐ATP (ATPase) enzyme were measured at the end of the experiment .The results showed that the maximal survival rate was observed in the water containing total amount of calcium and magnesium at 105 mg/L ,and the shrimp in the water containing total amount of calcium and magnesium at 70 mg/L had significantly higher grow th rate in body length than the shrimp in the water containing total amount of calcium and magnesium at 35 mg/L (P<0 .05) .There was significantly higher survival rate in the ratio of calcium to magnesium =1∶3 group than that in the ratio of calcium to magnesium = 1∶1 and 1∶4 groups (P<0 .05) .The findings indicate that significant increase in viability and survival is found in golden shrimp cultured in the water with total a‐mount of calcium and magnesium at 105 mg/L ,and ratio of calcium to magnesium=1∶3 .
		2015 Vol. (6): 364-369 [Abstract] ( 63 ) HTML (0 KB) PDF (781 KB) ( 363 )

	370	Effects of Vitamin C on Gene Expression of Transferrin during Early Development of Crucian Carp Carassius auratus gibelio
		XIONG Hualong,YAO Junjie,JIANG Zuoyu,AN Miao,ZHU Zhongsheng
		In order to discuss the gene expression characteristics of transferrin and effects of vitamin C so‐lution on the expression of transferrin genes during early development of crucian carp Carassius auratus gibelio .RT‐PCR and fluorescence quantitative PCR techniques were used to detect effect of vitamin C on the expression of transferrin genes and its mRNA expression levels during the early development of crucian carp .The results showed no expression of transferrin in mature eggs ,fertilized eggs and expanded blasto‐cyst stage ,while the expression of transferrin was determined in gastrula stage ,and the mRNA expression level of transferrin showed “up‐down‐up” trend ,and a peak in the heatbeating stage ,and the minimum in the prehatching stage .In the group of vitamin C (30 mg/L) ,the mRNA expression level of transferrin was lower than that in normal group during embryonic stage ,while higher than that in normal group during larval stage .The findings showed that the function of transferrin was independent from the binding iron function during embryonic development ,and appropriate vitamin C led to be decreased in the function of transferrin in immune during embryonic development ,and to be increased in the function of transport iron in yolk‐sac larval stage .
		2015 Vol. (6): 370-374 [Abstract] ( 110 ) HTML (0 KB) PDF (912 KB) ( 393 )

	375	Isolation,Identification and Antibiotic Susceptibility Testing of Plesiomonas shigelloides from Goldfish
		ZHANG Pei,ZHU Aihua,HU Xiucai,LAN Yun,LI Xue,SHEN Xiaojing,L Aijun
		A bacterium was isolated from hepatopancreas in goldfish (Carassius auratus)and named ZP‐1 . The isolate was identified by bacterial morphology ,physical and chemical characteristics ,16S rDNA se‐quencing and phylogenetic analysis .The results showed that ZP‐1 strain is Gram‐negative short‐rod shaped bacillus ,which was positive for acid production from glucose and lactose ,reduction of nitrate ,and indole production .The 16S rDNA of ZP‐1 strain was amplified by PCR ,and subcloned into pMD18‐T vector , whose sequencing was 1464 bp in length (accession number :KM458852) .Phylogenetic tree showed that strain ZP‐1 had high homology of 99 .80% with fish pathogenic Shiga Plesiomonas (KC825322) indicating that the isolate ZP‐1 was identified as P .shigelloides .The susceptibility testing was performed using 30 kinds of antibiotics ,and results showed that strain ZP‐1 was sensitive to cefotaxime ,cefixime ,cefaman‐dole ,gentamicin ,amikacin ,nalidixic acid ,chloramphenicol and ofloxacin .
		2015 Vol. (6): 375-379 [Abstract] ( 94 ) HTML (0 KB) PDF (723 KB) ( 305 )

	380	Molecular Identification of Two Snailfish,Liparis tanakae and L .zonatus in the Liaoning Coast based on COⅠ Gene Sequences
		LI Yulong,LIU Xiuze,DONG Jing,LI Yiping,GUO Dong,WANG Xiaolin
		Liparis tanakae and Liparis zonatus are two common snailfishes in the Bohai Sea and the Yellow Sea .A total of 20 individuals (9 L .tanakae individuals and 11 L .zonatus individuals) collected from Liaodong Bay were analyzed by sequence variations of mitochondrial CO Ⅰ gene fragments in this study . PCR amplification products of 620 bp COⅠ gene fragments were obtained ,with average contents of 22 .4%in A ,32 .2% in T ,26 .9% in C and 18 .5% in G .Sequence comparison of this segment revealed only two variant sites in the two species (one in L .tanakae individuals and two in L .zonatus individuals) with 2 transitions ,and no transversions and indels .The results showed that genetic distance of intra‐species of L . tanakae was ranged from 0 to 0 .2% and L .zonatus 0 to 0 .3% ,and the genetic distance among species was 0-0 .2% .The mean intra‐species distance was 0 .001 ,and the mean inter‐species distance between the two species was 0 .001 .Integrating morphological and mitochondrial DNA data ,L .tanakae and L .zonatus are considered as the same species of different morphs .
		2015 Vol. (6): 380-385 [Abstract] ( 75 ) HTML (0 KB) PDF (1025 KB) ( 193 )

	386	Phylogenetic Relationships among Members in Salmoninae and Genetic Variation amongDifferent Populationsof Rainbow Trout Farmedin China Basedon Cytb Sequence
		WANG Weiwei
		The Cytb sequences were amplified by PCR technique ,and genetic diversity was analyzed in different populations of rainbow trout Oncorhynchus mykiss farmed in China . The phylogenetic relationships among members in Salmoninae were established by comparing with the sequences from eleven other species representing five genera in subfamily Salmoninae .The results showed that the local rainbow trout population (0 .001 ) had the minimal intraspecies genetic distance ,and Donaldsons population the maximal (0 .004) .In the interspecies ,however ,there was the maximal genetic distance (0 .127) between taimen Hucho taimen and masou salmon O . masou ,followed by between taimen and coho salmon O . kisutch(0 .123)and the minimal genetic distances between Siberian char Salvelinus leucomaenis and brook trout S .fontinalis (0 .003) .Clustering analysis revealed that fish in the same genus was separately clustered ,lenok Brachymystax and H ucho being clustered together ,and Oncorhynchus and Salvelinus being clustered together .No clustering was observed in the individuals of different rainbow trout populations ,indicating that different populations of rainbow trout seem to have no significant genetic differentiation .
		2015 Vol. (6): 386-390 [Abstract] ( 64 ) HTML (0 KB) PDF (788 KB) ( 229 )

	391	Advances in Genes Related to Molting in Crustacean
		PU Hongshuang,GAO Xianggang,HOU Hongman,HE Chongbo,ZHANG Zhenyu,SHI Jingyun

		2015 Vol. (6): 391-398 [Abstract] ( 101 ) HTML (0 KB) PDF (656 KB) ( 348 )

	399	Function and Response to Envi ronmental Stresses of Glutathione Peroxidase in Aquatic Animals
		LU Kunlun,LIU Dandan,ZHANG Hongxu,KONG Xianghui

		2015 Vol. (6): 399-404 [Abstract] ( 55 ) HTML (0 KB) PDF (1180 KB) ( 254 )