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Information
月刊，1984年创刊
主 管：内蒙古自治区教育厅
主 办：包头医学院
主 编：赵云山
特邀主编：高长青
编辑出版：包头医学院学报编辑部
国际刊号：ISSN 1006－740X
国内刊号：CN 15－1182/R
邮发代号：16-292
Links
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2024 Vol. 43, No. 2
Published: 2024-03-25
Research and Application
Overview and Specific Topic
Research and Application
173
Effects of Soft Diet on Ovarian Development and Nutritional Quality of Adult Female Swimming Crab
Portunus trituberculatus
XIE Zhilong, JIANG Xiaodong, HOU Wenjie, LIU Meimei, WU Xugan
DOI: 10.16378/j.cnki.1003-1111.22061
The study was carried out in , About 5500 juvenile crabs of swimming crab
Portunus trituberculatus
[initial body weight of (3.12±0.54) g] were placed in 6 cement ponds (80 m×40 m×1.8 m). Every 3 cement ponds were set as a treatment, and the effects of soft diet and iced trash fish (ITF) on ovarian development and nutritional quality of swimming crab females were studied in 4 months. The results showed that: (1) hepatosomatic index (HSI), gonadosomatic index (GSI), and total edible yield (TEY) were 32.27%, 17.70%, and 8.69% higherin the soft diet groupthan those in the traditional diet group, respectively. But there were no significant differences among all groups (
P
>0.05). The proportion of super level crab and senior level crab in the soft diet group was 42.37% and 53.19% higher than that in the traditional diet group, respectively, and there was no significant difference among all groups (
P
>0.05). (2) The content of crude protein in the ovary and hepatopancreas was 21.41% and 31.32% higher in the soft diet group than that in the traditional diet group, respectively. The content in soft diet group was 12.30% and 9.05% lower than that in the traditional diet group. But there was no significant difference among groups (
P
>0.05). The content of biochemical composition in muscle was similar. (3) In terms of tissue color and the contents of total carotenoid, the red value (
a
*) and yellow value (
b
*) of the ovary was significantly higherin the soft diet groupthan that in the traditional diet group, but the difference was not significant (
P
>0.05); the total carotenoid content, and contents of astaxanthin, lutein, and β-carotene in the ovary and total carotenoid content in the hepatopancreas of soft diet group was significantly higher than that in traditional diet group (
P
<0.05). (4) In terms of fatty acid composition different tissues, the proportion of C22:6n3 in hepatopancreas and ovaries in soft diet group was significantly lower than that in traditional diet group (
P
<0.05), while the content of C18:2n6 was significantly higher in soft diet group than that in traditional diet group (
P
<0.05). The contents of amino acids in the muscle of the soft diet group were higher than those in the traditional diet group, but there was no significant difference (
P
>0.05). In conclusion, the soft diet can promote ovarian development and improve the proportion of crab with red paste and nutritional composition in edible tissues of female swimming crab.
2024 Vol. 43 (2): 173-188 [
Abstract
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179
)
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189
Application of Three Indigenous Bacterial Species in Aquaculture of American Eel
Anguilla rostrata
WEI Chengye, YANG Hongling, XIA Mengting, NIE Qingjie, ZHAI Shaowei, SUN Yunzhang
DOI: 10.16378/j.cnki.1003-1111.21236
In order to evaluate the possible application of three possible beneficial indigenous bacteria,
Bacillus paramycoides
MYα,
Acinetobacter variabilis
MYAⅡ and
Bacillus altitudinis
MY5 in American eel
Anguilla rostrata
, the three species bacteria were supplemented to the diets at dose of 5.0×10
7
cfu/g, and the effects on the growth performance, immune function and intestinal microbiota of American eel with an initial body weight of (10.00±0.09) g were determined. The results showed that American eel fed the diets containing MYα and MYAⅡ had increase in the weight gain rate (WGR) and specific growth rate (SGR), and the American eel fed the diets containing MY5 had the significantly lower hepatosmatic index (HIS) and viscerosomatic index (VSI) than the animals in the control group did (
P
＜0.05). The serum lysozyme activity was found to be increased significantly in fish fed MYAII and MY5 containing diets (
P
＜0.05), and the serum T-SOD activity in the group MYAII to be significantly higher than that in the control group (
P
＜0.05). The content of malondialdehyde (MDA) in liver of fish fed diets MYAⅡ and MY5 were lower than that in the control group. Compared with the control group, the abundance of genus
Lactobacillus
was increased and the abundance of genus
Brevinema
was decreased in groups MYα and MYAⅡ, but the abundance of
Escherichia
was increased in group MYα, indicating that there was more balanced intestinal microbiota in group MYAⅡ. On the other hand, the abundance of Mycoplasmataceae was increased significantly and
Lactobacillus
decreased in group MY5, indicating an imbalanced intestinal microbiota. In conclusion,
A. variabilis
MYAII led to improve the growth performance, immune function and antioxidant capacity, and modulate the intestinal microbiota, which implies that MYAII be a potential probiotic strain in the aquaculture of eel.
2024 Vol. 43 (2): 189-198 [
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112
)
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199
Transcriptome on Response of Four Species of Green Tide Algae to Heat Stress
ZHAO Hui, ZHOU Lingjie, CAI Chun′er, HE Peimin
DOI: 10.16378/j.cnki.1003-1111.22024
The Yellow Sea green tide has seriously damaged the marine ecology and caused huge economic loss and serious social influence. Four species of green tide algae
Ulva prolifera
,
U. linza
,
U. flexuosa
, and
U. compressa
were collected from the coast of Qingdao in July 2008, and the progeny remaining after pure line culture under suitable conditions were divided into two groups: the control group was treated at the optimum temperature and the treatment group was treated at 30 °C for 24 h. The algae were taken out, treated with liquid nitrogen and stored at -80 °C until RNA extraction, RNA quality control detection and transcriptome by high-throughput sequencing. It was found that the descending order of the number of differential genes in four
Ulva
species was expressed as
U. compressa
＞
U. flexuosa
＞
U. prolifera
＞
U. linza
. These differential genes were enriched in four metabolic pathways of transport and catabolism, lipid metabolism, amino acid metabolism and energy metabolism, while the differential genes in
U. prolifera
were enriched in pathways specific to oxidative phosphorylation, glyoxylate and dicarboxylic acid metabolism and ABC transporter, which may be related to the special response of
U, prolifera
to high temperature stress. The four types of
Ulva
all had complete heat shock protein synthesis and antioxidant systems, and the down-regulation of heat shock protein-related gene expression in
Ulva compressa
. Different
Ulva
resisted high temperature through the expression of different antioxidant enzymes, in which the role of superoxide dismutase was the most important. The finding provides theoretical basis for succession of four
Ulva
species during the outbreak of green tide.
2024 Vol. 43 (2): 199-214 [
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95
)
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215
Comparison of Individual Fecundity of Loach
Barbatula nuda
among Three River Systems in Liaoning Province
WU Chen, LUO Xiaonian, DUAN Youjian, LI Jiao, JI Chenyue, XING Yuxin, CHEN Long, YANG Yanyu
DOI: 10.16378/j.cnki.1003-1111.22059
In order to understand the individual fecundity of loach
Barbatula nuda
in Liaoning Province, the individual fecundity of loach was compared in 180 female loach with ovary at stage Ⅳ collected by traps with 0.5 cm mesh from Biliu River (E 122.51°, N 39.78°), Yalu River (E 123.72°, N 40.71°)and Liao River(E 123.34°, N 41.09°) by conventional biological method. The results showed that the loach had absolute fecundity of (6999.33±315.19) eggs, relative body length fecundity of of (85.06±2.98) eggs/mm and relative body weight fecundity of (1536.58±60.23) eggs/g. There were significantly higher relative fecundity of body length and body weight in Wanfu section of Biliu River than those in the other two river system (
P
<0.05). Most of the best fitting equations between individual fecundity and biological indices were found to be quadratic functions. The absolute fecundity and the relative fecundity in body length in the three river systems were significantly correlated with body length, body weight, net body weight, gonad weight and gonado-somatic index (
P
<0.01), and the relative fecundity of body weight was only significantly correlated with gonado-somatic index in Wanfu section of Biliu River and Tanghe section of Taizi River (
P
<0.01). A multiple stepwise regression analysis showed that the individual fecundity in Wanfu section of Biliu River and Tanghe section of Taizi River was closely related to gonad weight and gonado-somatic index, and significantly affected by net body weight and relative condition factor in Dapu section of Aihe River. The distribution of egg diameter showed that loach was a once spawning species a year. The finding further enriched the biological research data of
B. nuda
and provided basic data for its resource protection and sustainable utilization.
2024 Vol. 43 (2): 215-224 [
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105
)
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225
Estimated Size at First Sexual Maturity for Female Mantis Shrimp
Oratosquilla oratoria
in Bohai Bay Based on Gonado-Somatic Index (GSI)
XU Hailong, ZHENG Linlin, LIANG Qian, ZHAO Rongrong, XU Lili, ZHANG Dajuan
DOI: 10.16378/j.cnki.1003-1111.22078
In order to establish an objective method for research of body length of mantis shrimp
Oratosquilla oratoria
at the first maturity and accurately estimate it, a scientific survey of fishery resources was monthly conducted in the Bohai Bay from April to November 2019 (except for May), on the (20th±5th) day of each month by a single vessel bottom net with a width of 10 meters and an inner mesh size of 20 mm. The characteristics of mantis shrimp were measured, and immature and mature female individuals were determined based on the gonado-somatic index (GSI). The relationship between maturity ratio and kubo's standard body length was established to estimate body leng that initial maturity by a modified logistic with a variable asymptote (A). The results showed that the ranges of GSI for wet- and dried-female mantis shrimp were varied from 0.01% to 14.54% and from 0.01% to 24.11%, with the monthly mean of GSI decreases from the peak in April to the minimum in Aug, and then gradually increase during the survey period. The kubo′s standard length at first maturity was found to be (8.33±0.013) cm for wet female samples and (8.63±0.052) cm for dried-female samples. Accordingly, the flag criteria of the reproductive status were from 16% to 28% of the maximum registered wet-GSI and from 8% to 22% of the maximum registered dried-GSI, indicating that robust and stable enough to propose this method be applied to estimate the size of at first maturity and to enrich our knowledge about the relationship between the ratio of maturity and kubo′s standard length for female mantis shrimp. Using GSI to imply the physiological maturity of female mantis shrimp seems more sensible to compare with gonadal development stage. The varied percentages of the maximum obtained GSI of the dried samples present a wide range of the benchmark values to identify engagement into reproduction. The smaller number of individuals identified as reproductive is compensated by a lower asymptote value while increasing the flag values to high values
2024 Vol. 43 (2): 225-233 [
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] (
86
)
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234
Molecular Phylogenetic Relationships among 16 Species of Sweetlips Based on 3 Gene Sequences
CHENG Jing, ZHANG Guixin, CHEN Houhua, YANG Sen, LI Peiyuan, CHEN Jingyi, LIANG Rishen
DOI: 10.16378/j.cnki.1003-1111.21164
A total of 16 species of sweetlips were obtained, 14 species belonged to the genus
Plectorhinchus
, 2 species belonged to the genus
Diagramma
, domestic samples from the South China Sea and surrounding seas, while most of the foreign samples from the Indian Ocean, one in the South Pacific, and the mitochondrial Cyt b and nuclear RAG2 and S7 labeling sequences of these 16 fish species were obtained by PCR amplification and sequencing. The phylogenetic relationship of analytical molecules was constructed based on maximum likelihood method and Bayesian method. The results showed that the homologous sequence of 16 sweetlips in Cyt b genes were determined to be 657 bp, encoding 219 amino acids; the homologous sequences in RAG2 gene were 726 bp, encoding 243 amino acids, the homologous sequence in S7 genes were 729 bp, with numerous base insertions and deletions. The molecular phylogenetic tree were constructed using
Pomadasys maculatus
and
P. hasta
as outgroup species, sweetlips in the tree were divided into three major groups. Species in group Ⅰ possessed spectacular coloration and markings along bodies while species in group Ⅱ and group Ⅲ had monotone color and uniformly dark patterns, which were consistent with the morphological classification. Genus
Diagramma
did not form an independent branch but were clustered inside the
Plectorhinchus
group. Comparison of the dorsal spine numbers of
Diagramma
and
Plectorhinchus
with their relationships in the phylogenetic tree revealed that no correlation was found between dorsal spine numbers and species phylogenetic relationship, indicating that numbers of dorsal spines was used as an indicator to distinguish different sweetlips morphologically, but not be used as a basis to investigate the their relationships. The findings supported the viewpoints of most current molecular phylogenetic studies that genus
Diagramma
was classified into genus
Plectorhinchus.
2024 Vol. 43 (2): 234-242 [
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76
)
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243
Effects of Florfenicol Residues on Growth and Antioxidant System of
Chlorella
sp.
WANG Baolong, ZHANG Tao, ZHANG Weixiao, HUANG Ruipin, MA Pengfei, LI Na, ZHANG Yingqi, XUE Mingyu, ZHANG Qian, LIU Ying
DOI: 10.16378/j.cnki.1003-1111.22049
With the intensive development of aquaculture, antibiotic residues in aquaculture effluent posed a serious threat to aquatic ecosystem, which has aroused global attention. In present study, the commonly used diet microalga
Chlorella
sp. in aquaculture was taken as the research object to explore the effects of florfenicol on marine microalgae. The effects of florfenicol exposure at different concentrations for 72 h on the growth, the contents of pigments, reactive oxygen species (ROS), malondialdehyde (MDA) content and antioxidant enzyme activities of
Chlorella
sp. were analyzed. The results showed that florfenicol at low concentration promoted the growth of
Chlorella
sp., while inhibited the growth of
Chlorella
sp. at high concentration, and the contents of chlorophyll varied in a reverse U-shaped trend with the increase in mass concentration of florfenicol, with the maximal chlorophyll content in 0.100 mg/L florfenicol group, which was 74.6% higher than that in control group. Florfenicol exposure may cause the oxidative damage to the cell membrane of microalgae, 20 mg/L florfenicol led to the increase of MDA contents and stimulates the carotenoids synthesis, was 2.45 times higher than that in the control group, which may participate in the resistance to oxidative damage. Under the experimental concentrations, the activities of glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were significantly inhibited. Compared to
Isochrysis galbana
,
Chlorella
sp. has a high tolerance to florfenicol. The finding provides theoretical basis for the treatment of antibiotic residues in aquaculture effluent.
2024 Vol. 43 (2): 243-251 [
Abstract
] (
127
)
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252
Two Transfection Reagents of Epithelioma Papulosum Cyprinid Cell Transfection: Condition Optimization and Application
LI Shangqi, XU Ziming, ZHAO Ran, SUN Xiaoqing, ZHANG Yan, LI Jiongtang
DOI: 10.16378/j.cnki.1003-1111.22035
To solve no data support of reagent dose and sample time in gene overexpression research with epithelioma papulosum cyprinid(EPC) cell transfection, two transfection reagents, Lipofectamine
®
2000 and FuGENE
®
HD, were used to transfect a fluorescent protein tagged plasmids into EPC cells according to varied dose groups with transfection efficiencies recorded at multi-temperature and multiple time points. EPC cells were plated on 35mm cell plate at 28 ℃, with the maximal transfection efficiency (5.92±0.52)% when transfected by 16 μL Lipofectamine
®
2000 with 4 μg DNA after 48 h post transfection;meanwhile (9.81±0.33)% EPC cells were fluorescence positive by 12 μL FuGENE
®
HD with 4 μg DNA after 72 h post transfection. To test these optimized conditions and explore the transfection performance differences of the two reagents in transcriptional regulation research, an EGFP tagged overexpression vector was conducted which loaded a common carp transcription factor peroxisome proliferators-activated receptors alpha (PPARα), which was involved in highly unsaturated fatty acid biosynthesis. The transfection efficiency of Lipofectamine
®
2000 was found to be 4% and that of FuGENE
®
HD 8%. Then, real-time quantitative PCR was performed to detect the expression level of candidate target gene fatty acid desaturase 2 (fads2). The candidate target genes were similarly up-regulated by transcription factor overexpression despite transcriptional activation by the lipid from transfection reagent, compared with strict control groups transfected with no-load plasmids. In this study, the dose of two transfection reagents (Lipofectamine
®
2000 and FuGENE
®
HD) to DNA and their sampling time of EPC cell transfection were optimized, and tested with a transcriptional regulation instance of common carp, which contributed to effectively conduct gene research with EPC cells and better promoted breeding innovation of fishery germplasm resource.
2024 Vol. 43 (2): 252-263 [
Abstract
] (
100
)
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264
Genome-Wide Selection Signature of Yellow River Common Carp
Cyprinus carpio haematopterus
WANG Yanhui, ZHANG Qin, WANG Bingke, ZHANG Ling, YANG Xingli, ZHOU Xiaolin, FENG Jianxin
DOI: 10.16378/j.cnki.1003-1111.22097
It is speculated that there should be specific selection signatures in the genetic improvement population of Yellow River common carp
Cyprinus carpio haematopterus
arising from long-term artificial selection after domestication. Understanding and improvement of the signatures at a genetic level is important to inform future efforts to further improve a fish which is one of the most important commercial species in freshwater culture with a long period of artificial selection. In this study, two genetically modified populations: 12 whole sibling families (10) of the new strain of Yellow River common carp (F5) and the culture population of Furui carp No. 2 (FFRC No.2)and one unselected population were resequenced whole-genome, and a total of 8 665 728 high-quality SNP sites were obtained by typing data of SNP sites, and the selected genomic regions were detected by the fixation index (
F
st
), nucleotide diversity (π ratio)and cross-population composite likelihood ratio test (XP-CLR). Top 5% of values was used as the threshold signature of artificial selection where were annotated to further identify potential candidate genes. To further control the false positive rates of the detection of selection signatures, genes identified by three methods were used in further enrichment analyses. In total, compared with the reference population, 1434 and 1333 genes were potentially selected between two genetic improvement populations, respectively. These candidate genes were enriched in cell development, heart contraction, cell differentiation and protein glycosylation in new strain of Yellow River common carp. The candidate genes were enriched in embryonic skeletal joint development, membrane fusion and neurotransmitter secretion in
C. carpio
FFRC No.2 strain. Mixing two genetic improvement populations, compared with the reference population, 2037 genes were annotated, including Fabp2, Acaca, Acsl and Cpt1. These candidate genes were significantly enriched in several fatty acid metabolism-related pathways, including glycerophospholipid metabolism, fatty acid biosynthesis, fatty acid degradation and peroxisome proliferators activate receptors (PPAR) signaling pathway, which may partly explain the difference between the genetic improvement population and reference population in terms of growth traits. In the current study, a number of regions with signatures of positive selection were identified, and further functional analyses on these regions identified a list of genes involved in fatty acid metabolism. The findings provide a new insight into the mechanism of genetic improvement of growth traits in common carp and contribute to promote the application of genomic selection in breeding of common carp.
2024 Vol. 43 (2): 264-272 [
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111
)
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273
Genome-Wide Identification, Characterization and Expression of UROS Gene in Yesso Scallop
Patinopecten yessoensis
HANG Yunna, WANG Yiying, SUN Hongyan, FAN Zhiyue, WANG Xubo, TIAN Ying, HAO Zhenlin, MAO Junxia, CHANG Yaqing
DOI: 10.16378/j.cnki.1003-1111.22064
In order to explore the molecular function of uroporphyrinogen Ⅲ synthetase (UROS) gene in shell color formation, and provide more clues for the mechanism reseach of shell color in molluscs, the genome-wide identification, sequence characterization and expression of the UROS gene were investigated in 2 years old Yesso scallop
Patinopecten yessoensis
with brown and white shell colors, an important aquaculture species in North China by bioinformatic and quantitative real-time PCR methods. A single-copy of UROS gene, named PyUROS gene, was identified in the whole genome of the Yesso scallop, with full length of 68 808 bp containing 9 exons and 8 introns, and encoding 298 amino acids. A conserved HemD domain was predicted in the PyUROS protein sequence, which confirmed the identification of PyUROS gene. PyUROS gene was ubiquitously expressed in all the adult tissues of the Yesso scallop, indicating that the PyUROS gene had an important role in life activities of the Yesso scallop. Significantly different expression levels of PyUROS gene were found among different mantle regions and between different shell color individuals, which indicated that the key function of PyUROS gene was involved in shell coloration in the Yesso scallop. The finding will provide valuable information for molecular mechanism research of shell coloration, and lay a theoretical foundation for genetic breeding of the shell color trait.
2024 Vol. 43 (2): 273-280 [
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] (
115
)
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281
Expression and Identification of Glycoprotein of
Siniperca chuatsi
rhabdovirus
in Baculovirus Expression System
ZHANG Yanbing, YE Jiaxin, SUN Wei, LIU Xiaodan, LIN Qiang
DOI: 10.16378/j.cnki.1003-1111.21185
Siniperca chuatsi rhabdovirus
(SCRV) genome encodes five structural proteins, in which glycoprotein G is the main antigen on the surface of the virus, In order to achieve large expression of the antigen protein, the glycoprotein G of SCRV was inserted into Baculovirus shuttle vector pFastBac1 by molecular cloning technology and transformed into
Escherichia coli
DH5α competent cells. Then it was transformed into DH10Bac
E. coli
competent cells, and the recombinant plasmid rBacmid-G was obtained by blue-white spot and resistance screening. The recombinant bacmid was transfected into Sf9 insect cells by liposome transfection method to obtain recombinant Baculovirus expressing glycoprotein. The obtained P3 generation recombinant virus was infected with Sf9 insect cells for SDS-PAGE detection of the recombinant protein. After successful expression, the target protein was purified by nickel ion affinity chromatography column, and then the recombinant protein was identified by Western blot. There was a specific band at 50 ku, and no band in the control group, indicating that the prepared antigen can specifically bind to the antibody. Therefore, the SCRV glycoprotein G was successfully expressed in the Baculovirus expression system. The findings provide technical support for the development and large-scale production of SCRV glycoprotein G vaccine.
2024 Vol. 43 (2): 281-288 [
Abstract
] (
96
)
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166
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289
Analysis and Evaluation of Nutritional Composition in Muscle of
Selenotoca multifasciata
GAO Jin, HAN Lina, WANG Yongbo, CHEN Fuxiao, LIU Jinye, ZHU Hai, FU Shuyuan
DOI: 10.16378/j.cnki.1003-1111.22060
In order to clarify characteristics of the muscle quality and nutritional composition of
Selenotoca multifasciata
, the contents of main nutrients including amino acids, fatty acids and some of minerals were analyzed and evaluated in 10 g of dorsal and abdominal muscles in 15 marine cultured
S
.
multifasciata
[body weight of (481.5±78.3) g]. The results showed that there were (67.50±0.20)% of moisture, (18.60±0.33)% of crude protein, (12.20±0.08)% of crude fat and (1.40±0.12)% of crude ash in muscle of S.
multifasciata
. The detected 17 amino acids accounted for 57.01% of the total content in dry muscle tissue, including 22.55% of essential amino acids, with the first limiting amino acid of valine (Val) and the second limiting amino acid of methionine+cystine (Met+Cys). A total of 24 fatty acids were detected, including 8 saturated fatty acids (41.43%), 7 monounsaturated fatty acids (36.29%) and 9 polyunsaturated fatty acids (22.28%). In the 8 minerals, the macronutrient potassium and the trace element zinc were abundant at 3830.00 mg/kg and 6.80 mg/kg, respectively. The findings indicated that the essential amino acid composition of
S
.
multifasciata
is reasonably balanced, and rich in fatty acids and high in minerals, and has high nutritional value, which provides a nutritional theoretical basis for the promotion of the culture and diversification of the processing and utilization of
S
.
multifasciata.
2024 Vol. 43 (2): 289-296 [
Abstract
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127
)
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191
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297
Karyotype and Ploidy Analysis of
Gymnocypris selincuoensis
LI Xin, MA Kexin, GUO Wenxuan, LI Yifan, YANG Yueyao, ZHOU Ziyu, LIU Haiping, XIAO Shijun, ZHOU He
DOI: 10.16378/j.cnki.1003-1111.22116
Harsh plateau environment in Tibetan Plateau has unique and rich biological resources. The chromosomes in different groups and genera in the subfamily Schizothoracinae showed diverse characteristics.The chromosome samples were prepared from renal tissue cells of
Gymnocypris selincuoensis
with body weight of 246.0—374.2 g by the conventional cold drop method. Giemsa staining analysis was used to determine the chromosome number and karyotype. The band type and ploidy were speculated through Ag-NORs and Chromomycin A3/4,6-diamidino-2-phenylindole double fluorescent staining technology. It was found that the karyotype formula of
G. selincuoensis
was 4n=88, 40m+20sm+28st/t, NF=148. In interphase nucleoli, the maximal frequency of Ag-NORs sites was 4. In each metaphase karyotype, both Ag-NORs sites and CMA
3
-positive sites were shown to be 4 on the short arms of submetacentric chromosomes. In conclusion, different
G. selincuoensis
samples had identical chromosome numbers.
G. selincuoensis
is a genetic tetraploid with four sets of chromosomes. The finding provides an important basis for studying genetic relationships among species and reference for the subsequent research on the classification and evolutionary status of the subfamily Schizothoracinae.
2024 Vol. 43 (2): 297-303 [
Abstract
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82
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304
Analysis and Evaluation of Nutritional Compositions of
Barnea davidi
FU Zhiyu, LI Dacheng, BAI Yong'an, YU Di, YU Zhe, WANG Qingzhi, ZHENG Jie
DOI: 10.16378/j.cnki.1003-1111.22074
In order to fully understand nutritional compositions and edible value of
Barnea davidi
, the contents of proximate nutritive compositions, part of minerals, fatty acid and amino acid composition of siphon and visceral mass of
B
.
davidi
with body weight of (30.0±5.0) g collected from coastal Jinzhou in China were analyzed and evaluated by the corresponding national standard methods. The results showed that there was much higher crude protein content in siphon (75.45%) than that in visceral mass (47.03%), and much lower crude fat (1.68%), ash (15.23%) and total sugar (4.37%) than those in visceral mass. Only higher contents of sodium, cuprum and zinc were found in siphon than those in visceral mass. Both siphon and visceral mass contained 32 fatty acids, the percents of polyunsaturated fatty acids were 30.02% and 20.71%, respectively, with significantly higher perent of eicosapentaenoic acid (9.48%) and docosahexaenoic acid (14.97%) in siphon than those in visceral mass. A total of 18 amino acids were detected in both siphon and visceral mass, with the maximal contents of glutamate, aspartate and glycine.
B. davidi
had contents of essential amino acids (23.63 g/100 g) and flavor amino acids (28.50 g/100 g) in siphon, higher than those in visceral mass, with similar contents of essential and non-essential amino acids in siphon (53.43%) and visceral mass (56.59%). The first and second limited amino acids were shown to be tryptophan and methionine+cystine in siphon, which were determined by the amino acid score (AAS) and chemical score (CS). The essential amino acid index (EAAI) was found to be 66.38 in siphon and 50.14 in visceral mass. The siphon and visceral mass of
B. davidi
was characterized by high protein and low fat in nutrition composition, which provided the theoretical basis for the high value comprehensive development and utilization of
B. davidi
.
2024 Vol. 43 (2): 304-311 [
Abstract
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111
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312
Genetic Diversity Analysis for Wild and Cultured Filefish
Thamnaconus modestus
Populations Revealed by Microsatellite Markers
WANG Jiulong, YE Miao, LI Hongli, XU Wengang, LIU Liming, DU Rongbin, YIN Shuo
DOI: 10.16378/j.cnki.1003-1111.21165
Utilizing filefish
Thamnaconus modestus
complete genome sequence published in NCBI database, we screened microsatellite loci in the genome and designed primer pairs. Among 50 microsatellite loci selected randomly, 20 novel loci amplified target products efficiently and steadily and the 20 microsatellite markers were evaluated using a wild filefish population. The number of allele (Na) and effective allele (Ne) for these markers was found to be ranged from 4 to 13 and from 3.758 to 12.000 per locus, the observed heterozygosity (Ho) and expected heterozygosity (He) values were ranged from 0.267 to 0.833 and from 0.413 to 0.932, respectively, polymorphism information content (PIC) ranged from 0.361 to 0.910, and the Shannon-Weiner′s diversity index ranged from 0.806 to 2.520. Comparison of genetic diversity was conducted between the wild and cultured populations, the average Na and Ne were 8.2 and 7.3; the average Ne were 7.168 and 6.239; the average Ho and He were 0.663 and 0.561; the average PIC were 0.780 and 0.684; the average value of Shannon-Weiner′s diversity index were 1.914 and 1.647. The high-genetic diversity was shown to be maintained in the cultured population while polymorphism of the cultured population was lower than the wild, indicating that artificial breeding had a certain impact on the genetic diversity of the cultured population. The microsatellite markers developed in the present research could be used in population genetics and molecular marker-assisted selection for the filefish, and the findings provided theoretical basis of resources conservation and genetic breeding for
T. modestus
.
2024 Vol. 43 (2): 312-318 [
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92
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(969 KB) (
32
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Overview and Specific Topic
319
Research Progress on Staging and Identification Technology of Molting Cycle in Shrimp and Crab: a Review
HOU Yiling, CHENG Wenzhi, WEI Yiming, DENG Xiaojie, CHEN Chuanxi, MAO Yong
DOI: 10.16378/j.cnki.1003-1111.22058
2024 Vol. 43 (2): 319-332 [
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102
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333
Advances in Research on Effects of Hypoxic Stress in Fish
YU Xinxin, ZHENG Guodong, CHEN Jie, ZOU Shuming
DOI: 10.16378/j.cnki.1003-1111.21206
2024 Vol. 43 (2): 333-340 [
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183
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