Isolation and Identification of Pathogenic Bacteria and Antagonistic Bacteria in Loach Gill Haemorrhagic Disease
WANG Qiannan1, FENG Yan2, JIA Lingchen1, LIU Youhua1, PI Qiaomu1, XU Siqi1, LI Liantai1,2, CAI Yuefeng2, AN Xianhui1,2
1. Jiangsu Key Laboratory of Marine Bioresources and Eco-Environment, School of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang 222005, China; 2. Jiangsu Key Laboratory of Marine Biotechnology, Lianyungang 222005, China
Abstract：Loach gill haemorrhagic disease is a common disease in the process of loach Misgurnus anguillicaudatus breeding. In this study, the dominant pathogen JY5 was isolated from the lesion site, and then the antagonist CJT23 inhibiting the growth of JY5 was isolated from the bottom mud of the loach breeding ponds. According to the colony morphology observation, physiological and biochemical identification and 16S rDNA sequence analysis of JY5 and CJT23, the pathogen JY5 was preliminarily identified as Aeromonas veronii and the antagonist CJT23 was identified as Pseudomonas marginalis, which were tentatively named as A. veronii JY5 and P. marginalis CJT23. Further drug sensitivity test of strain CJT23 showed that the strain was sensitivity and medium sensitivity to 6 drugs including ciprofloxacin, norfloxacin, gentamicin, streptomycin, enrofloxacin and compound sulfamethoxazole. It is suggested that the above 6 antibiotics should be avoided when the strain CJT23 was used to control the gill hemorrhagic disease of loach. The antagonistic spectrum experiment showed that the strain CJT23 has different degrees of bacteriostasis to pathogenic bacteria A. veronii JY5, V. parahaemolyticus, V. anguillaris, V. vulnificus, A. hydrophila, A. sobria, and V. havieri, and the diameters of the bacteriostasis zones were (21.00±0.41) mm, (17.97±0.21) mm, (15.93±0.26) mm, (12.73±0.26) mm, (12.63±0.17) mm, (12.57±0.12) mm and (11.47±0.17) mm, without inhibition effect on A. caviae. The findings provide certain reference significance for the prevention and control of loach diseases, and for ecological control research.
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2022, Vol. 41 
