Isolation, Identification and Related Characteristics Analysis of Pathogen Isolated from Red Swamp Crayfish Procambarus clarkii with Tail-Rotted Disease
LIU Zhanghuai1,2, WU Ting2, WANG Jiajun1, ZHU Chunyan1, ZHANG Yingying1, MENG Qingguo2
1. Baoying Center for Control and Prevention of Aquatic Animal Infectious Disease, Yangzhou 225800, China; 2. Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Marine Science and Engineering, Nanjing Normal University, Nanjing 210023, China
Abstract：The purpose of this experiment was to study the species and related characteristics of pathogen which caused tail-rotted disease of red swamp crayfish Procambarus clarkii. Two representative bacterial strains with different colony morphologies and colors were isolated from infectious location of diseased red swamp crayfish, and known as LW2047-1 and LW2047-2. The pathogen bacteria were confirmed as strain LW2047-2 by artificial infection test. We observed strain LW2047-2 with bacteriological methods and analyzed that by morphological, physiological and biochemical characteristics. Finally, strain LW2047-2 was identified as Citrobacter freundii when 16S rRNA and gyrB gene were sequenced and analyzed. CFA gene and chi gene were detected for analysis of adhesion and chitin decomposition characteristics. Results of antimicrobial sensitivity test showed that strain LW2047-2 was sensitive to enrofloxacin and neomycin sulfate and insensitive to sulfa drugs.
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2022, Vol. 41 
