Abstract：A dominant bacterial strain NDLc-P was isolated from the liver of diseased large yellow croaker Larimichthys crocea in Ningde, Fujian Province. An artificial infection with the isolates caused the similar symptom in the fish, with 50% lethal concentration of 3.98×107 cfu/mL within 7 d trial. Physiological and biochemical tests by commercially produced kit systems (bioMerieux) revealed that strain NDLc-P was identified as Aeromonas hydrophila. Then, the 16S rDNA sequence analysis showed that the bacterium had a 99.87% identify with A. hydrophila. The phylogenetic analysis also showed that the NDLc-P isolate was clustered most clearly with A. hydrophila. Furthermore, healthy mice were immunized with formalin inactivated strain NDLc-P, and the ELISA showed that the titer of antisera was determined to be 40 000. These findings provided a reference for research on pathogenic characteristics, diagnosis and prevention and treatment of large yellow croaker disease caused by A. hydrophila.
[1]LIU M, DE MITCHESON Y S.Profile of a fishery collapse:why mariculture failed to save the large yellow croaker[J].Fish and Fisheries,2008,9(3):219-242. [2]农业农村部渔业渔政管理局,全国水产技术推广总站,中国水产学会.2020中国渔业统计年鉴[M].北京:中国农业出版社,2020:22. [3]曹飞飞,朱凝瑜,郑晓叶.2014—2016年浙江省大黄鱼养殖病害测报及防治建议[J].浙江农业科学,2017,58(6):1043-1047. [4]DADAR M, DHAMA K, VAKHARIA V N, et al. Advances in aquaculture vaccines against fish pathogens:global status and current trends[J].Reviews in Fisheries Science & Aquaculture,2017,25(3):184-217. [5]LI C C, YE Z C, WEN L Y, et al. Identification of a novel vaccine candidate by immunogenic screening of Vibrio parahaemolyticus outer membrane proteins[J].Vaccine,2014,32(46):6115-6121. [6]POLZ M F, CAVANAUGH C M. Bias in template-to-product ratios in multitemplate PCR[J].Applied and Environmental Microbiology,1998,64(10):3724-3730. [7]邹玉霞,张培军,莫照兰,等.大菱鲆出血症病原菌的分离和鉴定[J].高技术通讯,2004,14(4):89-93. [8]何亮,陈群,曾忠铭,等.通过特异PCR扩增和16S rDNA序列分析检测动弯杆菌[J].微生物学报,2005,45(1):27-30. [9]沈锦玉,余旭平,尹文林,等.养殖大黄鱼低温弧菌病病原的研究[J].水产学报,2004,28(增刊):130-134. [10]李忠琴,张坤,林茂,等.大黄鱼(Pseudosciaena crocea)致病性维氏气单胞菌的分离鉴定与药敏特性研究[J].海洋与湖沼,2017,48(1):139-147. [11]曹际,马林,张文畅,等.大黄鱼源溶藻弧菌的鉴定及其菌蜕制备[J].微生物学通报,2018,45(1):129-137. [12]秦莉,殷建国,张薇,等.白斑狗鱼(Esox lucius)致病性嗜水气单胞菌的分离与鉴定[J].渔业科学进展,2014,35(5):40-45. [13]SUAREZ G, KHAJANCHI B K, SIERRA J C, et al. Actin cross-linking domain of Aeromonas hydrophila repeat in toxin A (RtxA) induces host cell rounding and apoptosis[J].Gene,2012,506(2):369-376. [14]陆承平.致病性嗜水气单胞菌及其所致鱼病综述[J].水产学报,1992,16(3):282-288. [15]SAITANU K, WONGSAWANG S. Red-score disease in carp (Cyprinus carpio)[J].Journal of Aquatic Animal Health,1982,5(3):79-96. [16]蒋自立,李春涛,张其中,等.黄颡鱼败血症病原菌的分离鉴定与病理组织学观察[J].西南师范大学学报(自然科学版),2012,37(6):77-82. [17]梁利国,谢骏.青鱼病原嗜水气单胞菌分离鉴定、毒力因子检测及药敏试验[J].生态学杂志,2013,32(12):3236-3242. [18]杨宁,黄海,张希,等.尼罗罗非鱼嗜水气单胞菌病的病原分离鉴定和药敏试验[J].水产科学,2014,33(5):306-310. [19]曹军,冯学芝,冯展波,等.大黄鱼致病性嗜水气单胞菌的分离与鉴定[J].湖北农业科学,2007,46(5):808-810. [20]MU YN, DING F, CUI P, et al. Transcriptome and expression profiling analysis revealed changes of multiple signaling pathways involved in immunity in the large yellow croaker during Aeromonas hydrophila infection[J].BMC Genomics,2010,11(1):1-14. [21]姜艳丽,李丽丽.嗜水气单胞菌的研究进展[J].黑龙江农业科学,2015(2):142-146. [22]窦勇,宁喜斌.副溶血弧菌多克隆抗体的制备及其特性分析[J].食品与生物技术学报,2007,26(3):85-89. [23]李华,李重实,李明,等.抗鲶爱德华氏菌多克隆抗体的制备及特性分析[J].中国海洋大学学报(自然科学版),2010,40(10):29-32. [24]王雪妹.鳗弧菌多克隆抗体和单克隆抗体的制备及其特性分析[D].福州:福建农林大学,2015.
2022, Vol. 41 
