1. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China; 2. Jiangsu Institute of Marine Fisheries, Nantong 226007, China; 3. Nanjing Normal University, Nanjing 210023, China
Abstract：Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease (VpAHPND) was used as the test strain. Antagonistic strains were isolated and screened from the healthy Pacific white shrimp Litopenaeus vannamei collected from the coastal areas of Jiangsu Province, identified by morphological, physiological, biochemical and molecular biological methods, and their antagonistic effects and passive protection effects were evaluated. As a result, one antagonistic bacterial strain JSHY-R5 with good inhibitory effect against VpAHPND was selected, and identified as Enterococcus faecalis by morphological characteristics, physiological and biochemical and 16S rDNA sequence analysis. JSHY-R5 showed significant inhibitory effect on VpAHPND at 5∶1 inoculation ratio of JSHY-R5 to VpAHPND co-cultured. In vivo protection experiments, the survival rate of the healthy Pacific white shrimp challenged for 5-days by VpAHPND and exposed to JSHY-R5 group was increased by 26% compared to positive control group. The mRNA expression of V. parahaemolyticus adhesion factor VpadF was found to be decreased significantly in the shrimp(P<0.05). The mRNA expression levels of peneidins and hemocyanin in shrimp fed diet containing JSHY-R5 were shown to be significantly higher than those in the blank and positive group (P<0.05). The mRNA expression levels of Serpin in the probiotic and positive group were significantly higher than that in the blank group (P<0.05). The findings indicate that E. faecalis JSHY-R5 isolated inhibits VpAHPND infection, with characteristics of down-regulation of the mRNA expression of V. parahaemolyticus adhesion factor, and the mRNA expression of immune factors and enhancement of the host immunity to improve the survival rate of Pacific shite shrimp. JSHY-R5 has the potential to be used as feed additive in shrimp farming.
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2024, Vol. 43 
