Isolation and Identification of Pathogen of Bacterial Gill Disease in Brown Trout Salmo trutta
SUN Shuaijie1, ZHOU Jianshe2, WANG Wanliang2, CHEN Meiqun2, ZHANG Chi2, LI Ming1, TASHI Lhamo2
1. College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; 2. Institute of Fisheries Science, Academy of Agriculture and Animal Husbandry Sciences, Xizang Autonomous Region,Lhasa 850032, China
Abstract：In September 2020, gill disease of brown trout Salmo trutta was developed at a breeding base in Yadong County, Xigaze City, Xizang Autonomous Region. The sick fish had main clinical symptoms including open gill cover, gill filament congestion, lonely, and difficult breathing. In order to explore the cause of the disease and to find its treatment, a dominant strain (YDX-1) was isolated from the gill tissue of brown trout with gill disease, using conventional bacterial analysis, 16S rRNA gene sequence comparison analysis and artificial infection test. At the same time, the drug resistance of strain YDX-1 was explored. The results showed that the strain YDX-1 was a gram-negative bacterium, and its phylogenetic tree constructed based on the sequence alignment of 16S rRNA gene clusters with Aeromonas salmonicida, with the similarity of more than 99%. Combined with the morphological characteristics of the bacteria, biochemical properties and the results of sequencing identification, it was comprehensively identified as A. salmonicida. The artificial infection test showed that strain YDX-1 was infectious and had a higher lethality to brown trout. The results of the drug susceptibility test showed that strain YDX-1 was generally sensitive to 6 types (21 types) of drugs used, and was only insensitive to compound trimethoprim. The finding provides guiding significance for the prevention and treatment of bacterial gill disease of brown trout in Xizang.
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2023, Vol. 42 
