Abstract:Green microalga Dunaliella viridis has strong salt tolerance, can survive in 5.5 mol/L saturated salt solution, and has rich cellular material composition. It can be used as aquatic initial weaning food and health food. In this experiment, the green microalga was used as material and Walne's medium as culture substrate to compare the effects of different concentrations of natural plant growth regulator indole-3-acetic acid (IAA) and synthetic growth regulator 2,4-dichlorophenoxyacetic acid (2,4-D) as microalgae culture additives on the growth and biochemical components of the green microalga. The results showed that at the end of the culture period, the optimal application concentrations of indole-3-acetic acid and 2,4-dichlorophenoxyacetic acid were 0.2 mg/L and 0.05 mg/L respectively, the cell density was increased by 20.74% and 9.37% respectively compared with the control group, and the harvested biomass was 1.25 times and 1.09 times higher than that in the control group respectively (P<0.05). At the end of the culture period, the photosynthetic rate was increased first and then decreased with the increase in growth regulator concentration, and the respiratory rate increased. At the end of the culture period, the content of extracellular polysaccharide under the action of 0.20 mg/L indole-3-acetic acid was increased by 33.00%, the content of soluble protein under the action of 0.05 mg/L 2,4-dichlorophenoxyacetic acid was increased by 43.21%, and the content of glycerol under the action of 0.02 mg/L indole-3-acetic acid increased by 68.11%. Under the action of 0.20 mg/L indole-3-acetic acid and 0.50 mg/L 2,4-dichlorophenoxyacetic acid, the total lipid content was increased by 46.16% and 53.25% respectively compared with the control group, and the lipid yield was increased to 1.46 and 1.54 times as that in the control group, respectively. Considering the economic cost and ecological feasibility, 0.20 mg/L indole-3-acetic acid is more suitable as a plant growth regulator of the green microalga D. viridis.
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