Changes in Calcium Content, Histological Structure and Expression of Calreticulin Gene of Chinese Mitten Crab Eriocheir sinensis during Molting Cycle
YANG Hang, YANG Zhigang, ZHANG Long
1. Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; 2. Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN), Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; 3. National Demonstration Centre for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
Abstract：In this study, calcium ion contents in hepatopancreas and gill of Chinese mitten crab Eriocheir sinensis with average body weight of (5.63±2.35) g were determined during molting cycle including the molting stage (within 0.5 h after the old shell molting) and the late molting stage (0.5 h after the old shell molting until the new shell was incompletely hardened) by biochemical analysis. The structure changes in hepatopancreas and gill were histologically observed during the molting cycle by paraffin section and HE staining, and the expression of calreticulin (CRT) gene was analyzed in hepatopancreas and gill by fluorescence quantitative PCR. The results showed that there was higher calcium content in hepatopancreas in the postmolt, while lower in gill during the ecdysis and postmolt. The histological observation showed that the cell contents in hepatopancreas were changed obviously during the molting cycle. In the gill, the cavity structure of marginal vessel was not recovered at the edge of the gill lamellae. The fluorescence quantitative analysis showed that the expression levels of calreticulin gene was decreased in hepatopancreas and gill from the intermolt to premolt (minimum) and increased from the premolt to postmolt. In the ecdysis, the minimal expression level was observed in the hepatopancreas and gill, which provided a theoretical basis for the study of molting and calcium transport of Chinese mitten crab.
杨航, 杨志刚, 张龙. 蜕壳周期内中华绒螯蟹钙含量、组织结构及相关基因表达变化[J]. 水产科学, 2022, 41(1): 116-121.
YANG Hang, YANG Zhigang, ZHANG Long. Changes in Calcium Content, Histological Structure and Expression of Calreticulin Gene of Chinese Mitten Crab Eriocheir sinensis during Molting Cycle. Fisheries Science, 2022, 41(1): 116-121.
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2022, Vol. 41 
