Effect of Cu2+ on Antioxidant Capacity of Red Shell Color Breeding Line and Natural Population of Hard Clam Meretrix meretrix
TIAN Zhen1,2, ZHANG Zhidong1, CHEN Aihua1, WU Yangping1, CHEN Suhua1, ZHANG Yu1, CAO Yi1, LI Qiujie1,2
1. Jiangsu Institute of Marine Fisheries, Nantong 226007, China; 2. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
Abstract：Cu2+ as one of the main pollutants of heavy metal pollution in the ocean is characterized by wide source, easy residue, and easy enrichment of food chain, which constitutes a potential ecological crisis and economic crisis for the cultivation of marine shellfish. In order to understand the tolerance of natural populations Meretrix meretrix and red shell color breeding lines of hard cham to different concentrations of Cu2+, the effects of different concentrations of Cu2+ on the activities of superoxide dismutase (SOD) and catalase (CAT) and the relative expression of Cu/Zn-SOD gene in hard clam were studied. The results showed: (1) at the same concentration of Cu2+, the survival rate of red shell color breeding line hard clam was found to be slightly higher than that of natural population, without significant difference (P>0.05). (2) The changes in SOD and CAT enzyme activities of hard clam under different concentrations of Cu2+ showed the corresponding time effect relationship and concentration effect relationship, and the SOD enzyme activity of hard clam showed a linear upward trend, while CAT activity showed a first trend, with significant difference between red shell color breeding line and natural population (P<0.05). (3) The expression of Cu/Zn-SOD gene was consistent with the expression of SOD enzyme activity. With the linear expression of Cu2+ concentration, the maximal relative expression of gene was observed at Cu2+ concentration of 0.4 mg/L. (4) The antioxidant activity of red shell color breeding line hard clam was better than that of natural population. The purpose of this study is to lay a theoretical foundation for the selection of stress resistance and the development of new species of hard clam.
田镇, 张志东, 陈爱华, 吴杨平, 陈素华, 张雨, 曹奕, 李秋洁. Cu2+对文蛤红壳色选育系及自然群体抗氧化能力的影响[J]. 水产科学, 2022, 41(1): 62-68.
TIAN Zhen, ZHANG Zhidong, CHEN Aihua, WU Yangping, CHEN Suhua, ZHANG Yu, CAO Yi, LI Qiujie. Effect of Cu2+ on Antioxidant Capacity of Red Shell Color Breeding Line and Natural Population of Hard Clam Meretrix meretrix. Fisheries Science, 2022, 41(1): 62-68.
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2022, Vol. 41 
