Changes in Serum Bochemical Profiles and Osmotic Pressure of Schlegel's Black Rockfish Sebastes schlegelii Acclimatized by Various Salinities
CHENG Zhili1, SUN Pengfei1, LUO Jun1,2, SHEN Shuangye1, ZHANG Saisai3, WANG Wei1
1. Key Laboratory of Fish Applied Biology and Aquaculture in North China, Liaoning Province, Dalian Ocean University, Dalian 116023, China; 2. Dalian Sunasia Tourism Holding Co. , Ltd. , Dalian 116023, China; 3. Dalian Modern Agricultural Production Development Service Center, Dalian 116023, China
Abstract：Wild Schlegel's black rockfish Sebastes schlegelii with body weight of (94.32±6.41) g were reared in a tank from seawater with a salinity of 32 directly into seawater with a salinity of 30, 25, 20, 15, 10, 5, 4, 3, 2 and 0; in the chronic salinity acclimation, Schlegel's black rockfish were reared in the tank slowly into water with a salinity of 32, 30, 27, 24, 21, 18, 15, 12 and 9, with decrease in salinity at a rate of 1 salinity every 7 days. Then the semi-lethal salinity, osmotic pressure and the changes in serum biochemical profiles were determined in the fish samples collected at 14:00 on the first day. The median lethal concentrations of acute salinity in Schlegel's black rockfish for 24h was shown to be 0.29, with safe concentration 0.04. Under the condition of chronic salinity acclimation, the minimum osmotic pressure was (0.17±0.01) mOsm/kg at salinity 3, significantly different from that in other groups (P<0.05). There were peak activities of glutamic-oxaloacetic transminase (GOT) [(68.67±3.51) U/L], glutamic-pyruvic transminase (GPT) [(18.67±3.06) U/L] and lactate dehydrogenase (LDH) [(726±27) U/L] in the Schlegel's black rockfish exposed to a salinity of 24. The significant difference in urea content was observed between salinity groups and seawater control group (P<0.05), with a downward trend with the decreasing salinity. Serum total protein (TP) and serum albumin (ALB) contents had some fluctuation with the decrease in salinity, with the minimal TP at a salinity of 21, significantly different from the seawater control group (P<0.05). The minimal blood glucose value was found in a salinity of 21, without significant difference between salinity groups and seawater control group (P>0.05). The white cell ratio was not fluctuated greatly with the decrease in salinity in each salinity group, without significant difference between salinity groups and seawater control group (P>0.05).
成智丽, 孙鹏飞, 罗珺, 沈双烨, 张赛赛, 王伟. 盐度驯化下许氏平鲉血清生化指标及渗透压的变化[J]. 水产科学, 2021, 40(3): 310-317.
CHENG Zhili, SUN Pengfei, LUO Jun, SHEN Shuangye, ZHANG Saisai, WANG Wei. Changes in Serum Bochemical Profiles and Osmotic Pressure of Schlegel's Black Rockfish Sebastes schlegelii Acclimatized by Various Salinities. 水产科学, 2021, 40(3): 310-317.
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2021, Vol. 40 
