In Vitro Effect of Single and Compound Chinese Herbal Medicines on Bacteriostasis of Aeromonas hydrophila
TAO Sha1,2, ZHANG Xiaoping1,3, SHANG Baodi1,3, LI Xiaoyi1,3, YAO Junjie2
1. Guizhou Fisheries Research Institute, Guiyang 550025, China; 2. Center of Fisheries Resources and Environment, Guizhou University, Guiyang 550025, China; 3. Guiyang Special Aquatic Engineering Technology Center, Guiyang 550025, China
Abstract：To effectively control the economic loss caused by infection of Aeromonas hydrophila from channel catfish Ictalurus punctatus, 41 kinds of extracts of Chinese herbal medicine were obtained by decoction, such as Flos Lonicerae, Spica Prunellae and Pericarpium Granati. The diameter of inhibition circle, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of 41 kinds of Chinese herbal medicine and their compound to A. hydrophila were detected respectively. The results showed that 14 of 41 Chinese herbs had bacteriostasis circle, with the minimal MIC in Rhizoma Coptidis at concentration of 7.81 mg/mL, comprehensive diameter of inhibition circle, MIC and MBC, comprehensive screening of 6 Chinese herbs with better bacteriostatic effect: Pericarpium Granati, Fructus Chebulae, Herba Violae, Lignum Sappan, Rhizoma Coptidis and Fructus Mume. Drug combination results showed that there was no antagonism of 93.3% between the 6 herbs, with positive effect of 80%. The results of 25 groups according to preparation tables of mixed Chinese herbal medicine showed that M-10 compound group with V (Pericarpium Granati)∶V (Fructus Chebulae)∶V (Herba Violae)∶V (Lignum Sappan)∶V (Rhizoma Coptidis)∶V (Fructus Mume) = 2∶5∶1∶2∶3∶4 had the best comprehensive bacteriostatic effect. The findings provide a theoretical basis for the application of Chinese herbal medicine in bacterial diseases of A. hydrophila.
陶莎, 张效平, 商宝娣, 李小义, 姚俊杰. 中草药及复方对嗜水气单胞菌体外抑菌作用研究[J]. 水产科学, 2022, 41(5): 883-890.
TAO Sha, ZHANG Xiaoping, SHANG Baodi, LI Xiaoyi, YAO Junjie. In Vitro Effect of Single and Compound Chinese Herbal Medicines on Bacteriostasis of Aeromonas hydrophila. 水产科学, 2022, 41(5): 883-890.
2022, Vol. 41 
