Genetic Diversity Analysis of 14 Cultured Populations of Red Swamp Crayfish Procambarus clarkii in China
LIU Xiaoyu1,2, XIONG Lijing1,2, PENG Bo1,2, LUO Cuirong1,3, PENG Guohui1,2, ZHU Xi1,2, BAI Xufeng1,2,4,5
1. National Key Laboratory of Crop Genetic Improvement, Shuangshui Shuanglü Institute, Huazhong Agricultural University, Wuhan 430070, China; 2. College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China; 3. College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 4. Hubei Hongshan Laboratory, Wuhan 430070, China; 5. Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Wuhan 430070, China
Abstract:To assess the genetic diversity of cultured populations of red swamp crayfish Procambarus clarkii, 14 populations, 464 samples, of red swamp crayfish, were collected from Jiangxi, Zhejiang, Guangdong and Hubei provinces in China. The number of alleles, expected heterozygosity (He), observed heterozygosity (Ho), polymorphism information content (PIC), Hardy-Weinberg equilibrium, genetic distance and population structure of red swamp crayfish were analyzed by the 35 simple sequence repeat(SSR) markers. The results showed that there were 2—3 alleles for each SSR marker site; the values of PIC, Ho and He were arranged from 0.33 to 0.43, 0.32 to 0.41, and 0.36 to 0.44, respectively. Absent heterozygote in each marker locus resulted in the loss of Hardy-Weinberg equilibrium. Four genetic populations can be grouped according to the genetic distance and population structure of the 14 populations,and the populations from Xiangshuba, Wenzhou, Quzhou, and Jian, Tianhuangping, Dipuwere classified into the two independent clades,respectively. The population from Longnan and the other populations were clustered into another two clades,respectively. The findings indicate that the cultured populations of red swamp crayfish in this study only show a medium genetic diversity, hence, the collection and protection of the wild crayfish germplasm with a higher genetic diversity will play an important role in the genetic breeding.
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