复方中草药制剂对克氏原螯虾抗白斑综合征感染研究

doi:10.16378/j.cnki.1003-1111.22043

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摘要为评价饲料中添加大黄、黄芩、茵陈、地锦草、白术、板蓝根制成的复方中草药制剂对克氏原螯虾非特异免疫、抗氧化能力和抗白斑综合征病毒感染的效果,在空白饲料中分别添加0.5%、1.0%、1.5%和2.0%的复方中草药连续投喂克氏原螯虾14 d,分析克氏原螯虾血清非特异免疫酶活、肝胰腺抗氧化能力及其对白斑综合征病毒抗感染能力。试验结果显示,以摄食空白饲料的克氏原螯虾为对照,摄食复方中草药制剂的克氏原螯虾血清溶菌酶、酚氧化酶、酸性磷酸酶和碱性磷酸酶的活性分别增加了4.07%~16.20%(P<0.05)、12.96%~31.94%(P<0.05)、32.36%~39.87%(P<0.05)、36.50%~71.94%(P<0.05),肝胰腺总超氧化物歧化酶、过氧化氢酶和总抗氧化能力分别提升了4.39%~25.76%(P<0.05)、32.85%~93.43%(P<0.05)、21.52%~55.10%(P<0.05),肝胰腺丙二醛含量降低了26.22%~45.05%(P<0.05)。此外,复方中草药制剂处理的克氏原螯虾人工感染白斑综合征病毒5 d的存活率较对照组提高了13.33%~31.67%(P<0.05)。试验结果表明,在饲料中添加复方中草药制剂具有显著提升克氏原螯虾非特异免疫能力、抗氧化能力和抗白斑综合征病毒感染的能力,且1.0%添加量的效果最佳。

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关键词 ：复方中草药制剂, 非特异免疫, 抗氧化能力, 白斑综合征, 克氏原螯虾

Abstract：In order to evaluate the non-specific immunity, antioxidant capacity and anti-white spot syndrome virus (WSSV) infection effect of supplementation with compound Chinese herb medicine prepared byrhubarb root and rhizome, baical skullcap root, virgate wormwood herb, creeping euphorbia, largehead atractylodes rhizome, and isatis rootin red swamp crayfish Procambarus clarkiiin diet, juvenile red swamp crayfish with body weight of (8.94±1.16)g was reared in a 2 m×3 m×1 m aerated pond at stocking rate of 120 individuals per pond and fed the basic diet (control group) and supplemented with the compound Chinese herb medicine at a dose of 0.5%, 1.0%, 1.5% and 2.0% for 14 d. Then the non-specific immunity of hemolymph and the antioxidant capacity of hepatopancreas were determined, and the anti-infection ability of the red swamp crayfish against WSSV infection was analyzed. The results showed that the activities of lysozyme, phenol oxidase (PO), acid phosphatase (ACP) and alkaline phosphatase (AKP) in the serum of the red swamp crayfish fed the compound Chinese herb medicine were found to be increased by 4.07%-16.20% (P<0.05), 12.96%-31.94% (P<0.05), 32.36%-39.87% (P<0.05) and 36.50%-71.94% (P<0.05). The total superoxide dismutase (T-SOD), catalase (CAT) and total antioxidant capacity (T-AOC)in hepatopancreas were increased by 4.39%-25.76% (P<0.05), 32.85%-93.43% (P<0.05) and 21.52%-55.10% (P<0.05), and the malondialdehyde content in hepatopancreas was decreased by 26.22%-45.05% (P<0.05). The red swamp crayfish treated with compound Chinese herb medicine and then challenged with WSSV had increase insurvival rate by 13.33%-31.67% during the 5 d (P<0.05).The findings indicated that the addition of dietary compound Chinese herb medicine significantly enhanced the non-specific immunity, antioxidant capacity and resistance to WSSV infection in red swamp crayfish, with optimal dose of 1.0%.

Key words： compound Chinese herbs medicine preparation non-specific immunity antioxidant capacity white spot syndrome Procambarus clarkii

收稿日期: 2022-05-15

PACS:

S945.4

基金资助:河南省科技厅科技攻关项目(232102321030);河南省现代农业产业技术体系建设专项资金资助项目(HARS-22-16-G3);江苏省自然科学基金资助项目(BK20210943);江苏省水产动物营养重点实验室开放课题(KJS2228);盐城工学院校企合作项目(YG2021040909).

通讯作者: 任胜杰(1989—)男,博士;研究方向: 水产动物营养疾病调控. E-mail: Renshengjie1990@163.com.

作者简介: 田海军(1980—)男,副教授,硕士;研究方向: 水生动物病理与病害. E-mail: thj_666@163.com.

引用本文:

田海军, 任胜杰, 杨治国, 顾夕章. 复方中草药制剂对克氏原螯虾抗白斑综合征感染研究[J]. 水产科学, 2024, 43(1): 129-135.
TIAN Haijun, REN Shengjie, YANG Zhiguo, GU Xizhang. Effect of Compound Chinese Herb Medicine on Resistance to White Spot Syndrome Infection in Red Swamp Crayfish Procambarus clarkii. 水产科学, 2024, 43(1): 129-135.

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http://www.shchkx.com/CN/10.16378/j.cnki.1003-1111.22043 或 http://www.shchkx.com/CN/Y2024/V43/I1/129

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