Abstract：Morphological index and COⅠgene sequencing for species verification in wild Chinese minnow Rhynchocypris oxycephalus in Guanshan Geopark, Henan Province, and the nutritional composition and quality of Chinese minnow was evaluated by the analysis of conventional nutrients method and gas chromatography analysis method. The results showed that there were moisture of (79.59±2.92)%, crude protein of (16.62±2.73)%, crude fat of (1.15±0.45)%, ash of (0.93±0.05)%, pH of 6.91±0.16 and dressing rate of (61.50±3.35)%. A total of 17 kinds of amino acids were found in the muscle of Chinese minnow, with essential amino acid index (EAAI) of 96.24, the amino acid score (ASS) standard close to or greater than 1, the chemical score (CS) standard greater than 0.5, and the lysine content of 705 mg/g, which was 2.07 times as that as the FAO/WHO score and 1.60 times as that as the whole egg protein mode. There were 11 kinds of fatty acids in dorsal muscle, abdominal muscle, liver, intestine, brain, kidney and spleen of Chinese minnow. The content of EPA+DHA was 18.07% and PUFA 39.21% in muscle. The findings indicate that Chinese minnow in Henan Province is high in protein and PUFA (especially EPA and DHA), which can be used as high quality food source.
[1] ZHANG Z C, CHENG Q Q, GE Y S. The complete mitochondrial genome of Rhynchocypris oxycephalus (Teleostei:Cyprinidae) and its phylogenetic implications[J].Ecology and Evolution,2019,9(13):7819-7837. [2] 李筝.尖头大吻鱼岁HSPs基因克隆及热应激下HSPs和免疫相关基因的表达响应[D].新乡:河南师范大学,2018:9-10. [3] LIANG Y Y, SUI X Y, CHEN Y F, et al. Life history traits of the Chinese minnow Rhynchocypris oxycephalus in the upper branch of Yangtze River, China[J].Zoological Studies,2014,53(1):1-10. [4] STARNES L B, STARNES W C. Biology of the blackside dace Phoxinus cumberlandensis[J].American Midland Naturalist,1981,106(2):360-371. [5] 储玲,叶娟,司春,等.黄山九龙峰保护区尖头鱼岁的年龄、生长和繁殖(英文) [J].水生生物学报,2015,39(1):29-37. [6] SAKAI H, ITO Y, SHEDKO S V, et al. Phylogenetic and taxonomic relationships of northern Far Eastern phoxinin minnows, Phoxinus and Rhynchocypris (Pisces, Cyprinidae), as inferred from allozyme and mitochondrial 16S rRNA sequence analyses[J].Zoological Science,2006,23(4):323-331. [7] LEROY B, DIAS M S, GIRAUD E, et al. Global biogeographical regions of freshwater fish species[J].Journal of Biogeography,2019,46(11):2407-2419. [8] YU D, CHEN M, ZHOU Z C, et al. Global climate change will severely decrease potential distribution of the East Asian coldwater fish Rhynchocypris oxycephalus (Actinopterygii, Cyprinidae)[J].Hydrobiologia,2013,700(1):23-32. [9] 李其芳,严云志,储玲,等.太湖流域河流鱼类群落的时空分布[J].湖泊科学,2016,28(6):1371-1380. [10] 张春光,赵亚辉,邢迎春,等.北京及其邻近地区野生鱼类物种多样性及其资源保育[J].生物多样性,2011,19(5):597-604. [11] YU D, CHEN M, TANG Q Y, et al. Geological events and Pliocene climate fluctuations explain the phylogeographical pattern of the cold water fish Rhynchocypris oxycephalus (Cypriniformes:Cyprinidae) in China[J].BMC Evolutionary Biology,2014,14(1):1-12. [12] KUMAR S, STECHER G, LI M, et al. MEGA X:molecular evolutionary genetics analysis across computing platforms[J].Molecular Biology and Evolution,2018,35(6):1547-1549. [13] 马玲巧,李大鹏,田兴,等.1龄黄颡鱼的肌肉营养成分及品质特性分析[J].水生生物学报,2015,39(1):193-196. [14] 中华人民共和国卫生部.GB 5009.3—2016,食品安全国家标准 食品中水分的测定[S].北京:中国标准出版社,2016. [15] 中华人民共和国卫生部,中国国家标准化管理委员会.GB/T 5009.6—2016,食品中脂肪的测定[S].北京:中国标准出版社,2016. [16] 中华人民共和国国家卫生和计划生育委员会.GB 5009.4—2016,食品安全国家标准 食品中灰分的测定[S].北京:中国标准出版社,2017. [17] 中华人民共和国卫生部,中国国家标准化管理委员会.GB/T 5009.5—2016,食品中蛋白质的测定[S].北京:中国标准出版社,2016. [18] 隋修磊.尖头大吻鱼岁营养成分分析及品质评价[D].新乡:河南师范大学,2019:20-23. [19] 王光亚,中国预防医学科学院营养与食品卫生研究所.食物成分表:全国代表值[M].北京:人民卫生出版社,1991. [20] Energy and Protein Requirements. Report of a joint FAO/WHO ad hoc expert committee. Rome, 22 March-2 April 1971 [J]. FAO Nutrition Meetings Report Series, 1973,52:1-118. [21] FOLCH J, LEES M, STANLEY G H S. A simple method for the isolation and purification of total lipids from animal tissues[J].Journal of Biological Chemistry,1957,226(1):497-509. [22] ULBRICHT T L V, SOUTHGATE D A T. Coronary heart disease:seven dietary factors[J].The Lancet,1991,338(8773):985-992. [23] 陈宜瑜.中国动物志 硬骨鱼纲 鲤形目(中卷) [M].北京:科学出版社,1998. [24] MAHBOOB S, AL-GHANIM K A, AL-BALAWI H F A, et al. Study on assessment of proximate composition and meat quality of fresh and stored Clarias gariepinus and Cyprinus carpio[J].Brazilian Journal of Biology,2019,79(4):651-658. [25] 周朝伟,雷骆,邓星星,等.乌鳢与白乌鳢肌肉营养成分分析与评价[J].淡水渔业,2018,48(3):83-89. [26] 朱冰,樊佳佳,白俊杰,等.金草鱼肌肉品质和营养成分分析及评价[J].海洋渔业,2017,39(5):539-547. [27] 李池陶,关海红,胡雪松,等.大头鲤、黑龙江鲤、德国镜鲤及其杂种F3肌肉品质的比较[J].水产学报,2008,32(1):45-50. [28] 严安生,熊传喜,钱健旺,等.鳜鱼含肉率及鱼肉营养价值的研究[J].华中农业大学学报,1995,14(1):80-84. [29] 王桂芹,孙丽,李子平,等.不同食性鱼类的含肉率及肌肉营养组成与评价[J].饲料工业,2010(增刊):94-98. [30] 樊佳佳,朱冰,白俊杰,等.白金丰产鲫含肉率及肌肉营养成分分析[J].大连海洋大学学报,2018,33(3):347-352. [31] 王苗苗,王海磊,罗庆华,等.鳡鱼肌肉营养成分测定及评价[J].食品科学,2014,35(15):238-242. [32] 黄钧,杨淞,覃志彪,等.云斑鮰、泥鳅和瓦氏黄颡鱼的含肉率及营养价值比较研究[J].水生生物学报,2010,34(5):990-997. [33] 陈玉珍,唐黎,申晓东,等.白斑狗鱼含肉率及肌肉营养成分分析[J].水产科学,2010,29(10):578-582. [34] 杨四秀,蒋艾青.斑鳢含肉率及肌肉营养成分的测定[J].水产科学,2008,27(12):662-664. [35] 胡玖,张中英,吴福煌.尼罗罗非鱼与莫桑比克罗非鱼的含肉率及鱼肉的生化分析[J].淡水渔业,1982,12(4):34-37. [36] 陈定福,何学福,周启贵.南方大口鲶和鲶鱼的含肉率及鱼肉的营养成分[J].动物学杂志,1990,25(1):7-9,2. [37] 张昌吉,刘哲,王世银.虹鳟含肉率及肌肉营养成分分析[J].水利渔业,2006,26 (4):83-85. [38] 李文倩.鳜鱼、草鱼肌肉品质评价及比较[D].上海:上海海洋大学,2010. [39] HAARD N F. Control of chemical composition and food quality attributes of cultured fish[J].Food Research International,1992,25(4):289-307. [40] 陈小雷,崔凯,周蓓蓓,等.山泉流水与池塘养殖草鱼营养成分比较[J].水产科学,2020,39(1):63-71. [41] 蔡宝玉,王利平,王树英.甘露青鱼肌肉营养分析和评价[J].水产科学,2004,23(9):34-35. [42] 马旭婷,钱攀,戴志远.美国鳙鱼和鲢鱼的营养成分分析与评价[J].中国食品学报,2016,16(11):273-280. [43] 孙中武,尹洪滨.六种冷水鱼肌肉营养组成分析与评价[J].营养学报,2004,26(5):386-388. [44] 宋超,庄平,章龙珍,等.野生及人工养殖中华鲟幼鱼肌肉营养成分的比较[J].动物学报,2007,53(3):502-510. [45] CAI W C, LIU W B, JIANG G Z, et al. Lysine supplement benefits the growth performance, protein synthesis, and muscle development of Megalobrama amblycephala fed diets with fish meal replaced by rice protein concentrate[J].Fish Physiology and Biochemistry,2018,44(4):1159-1174. [46] 王思宇,郑永华,唐洪玉,等.灰裂腹鱼肌肉营养分析与评价[J].淡水渔业,2018,48(2):80-86. [47] 王雪锋,涂行浩,吴佳佳,等.草鱼的营养评价及关键风味成分分析[J].中国食品学报,2014,14(12):182-189. [48] 刘军,胡兵,李惠,等.长江上游四种特有鱼类肌肉营养组成与评价[J].水生生物学报,2007,31(5):763-766. [49] CEJAS J R, ALMANSA E, VILLAMANDOS J E, et al. Lipid and fatty acid composition of ovaries from wild fish and ovaries and eggs from captive fish of white sea bream (Diplodus sargus)[J].Aquaculture,2003,216(1/2/3/4):299-313. [50] 吕耀平,曹明富,姚子亮,等.花鱼骨和唇鱼骨的含肉率及肌肉营养成分分析[J].水生生物学报,2007,31(6):843-848. [51] 关健,柳学周,翟毓秀,等.褐牙鲆(♀)×犬齿牙鲆(♂)杂交F1及其亲本肌肉营养成分分析与比较[J].中国水产科学,2007,14(7):41-47. [52] 吴时敏.脂肪酸的膳食平衡研究进展[J].中国食物与营养,2000,6(6):48-49. [53] 张凤枰,宋军,张瑞,等.养殖南方大口鲶肌肉营养成分分析和品质评价[J].食品科学,2012,33(17):274-278.
2022, Vol. 41 
