Abstract：The coelomic fluid of echiuran worm Urechis unicinctus with body weight of (18.03±1.35) g was centrifuged at 4 ℃, 3000 r/min (centrifugal radius of 13.5 cm) for 20 min, and the supernatant was taken, and then centrifuged the blood cell pellet by centrifugation. After resuspending in sterile normal saline, it was crushed by ultrasonic disintegrator at 4 ℃ for 5 min, and the supernatant was taken as the supernatant of the coelomic cell crushing liquid. The antibacterial effects of the coelomic fluid supernatant, and coelomic cell crushing liquid on Aeromonas hydrophila, Escherichia coli, Vibrio parahemolyticus, V. anguillarum, A. veronii, V. harveyi, Staphylococcus aureus, and Micrococcus lysodeikticus were determined by the methods of Oxford cup and bacterial growth curve determination. Meanwhile, the influence of environmental factors including temperature, salinity, air exposure and fast on the antibacterial activity of coelomic fluid of the echiuran worm were investigated. The results showed that coelomic fluid supernatant had significant inhibiton on V. anguillarum and V. parahaemolyticus (P<0.05), while the coelomocyte ocytelysate supernatant had a significant bacteriostatic effect on V. anguillarum, V. parahaemolyticus and A. fragilis (P<0.05). There was slightly higher significant inhibition in the supernatant of the coelomic cell crushing liquid in the treatment group of salinity 25 than that in salinity 20 and 30 groups. However, the antibacterial activity of the coelomic cell crushing supernatant was found to be decreased significantly at salinity of 15 and 35, low salinity (15) showing more significant effect than at high salinity (35) (P<0.01). The coelomic cell crushing supernatant led to higher bacteriostatic effect under the temperature of 22—26 ℃, while the inhibition effect was declined significantly with the temperature below than 12 ℃ or more than 26 ℃ (P<0.01). The significantly lower antibacterial activity was observed in the coelomic fluid of echiuran worm exposed to the stress of air exposure for 8 h or more (P<0.05), and to be fasten for 2 days or more (P<0.01).
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2022, Vol. 41 
