Effects of Glyphosate-Resistant Genetically Modified Soybean on Antioxidant Capacity, Biochemical and Immune Indices of Jian Carp Cyprinus carpio var. jian
1. Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN), Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; 2. Shandong Vocational and Technical College of Commerce, Jinan 250103, China; 3. Shandong Provincial Key Laboratory of Storage and Transportation Technology for Agricultural Products, Jinan 250103, China; 4. Guangdong Ocean University, Zhanjiang 524000, China
Abstract：In order to evaluate the safety of glyphosate-resistant genetically modified soybeans (GMS) for Jian carp Cyprinus carpio var. jian, 480 juveniles with average body weight of (60±10) g were used as the test subjects in the experiment and randomly divided into four groups. The test diet was supplemented with 15% and 30% glyphosate-resistant GMS pretreated with high temperature, respectively, and the same proportion of non-GMS diet pretreated with high temperature was used as control. The feeding period was 180 d. Growth indicators were determined by conventional methods, and related hepatopancreas and blood indicators were determined by visible light method and dry powder method. The results showed that the addition of 15% and 30% glyphosate-resistant GMS to the diet did not led to significant effect on the growth index, organ index, and muscle routine nutritional components of carp (P>0.05). The activity of GSH-Px in hepatopancreas was significantly increased when the amount of GMS in the diet was 15% and 30% (P<0.05). The activity of CAT in hepatopancreas was significantly reduced and the level of MDA in hepatopancreatic was significantly increased by adding 30% GMS (P<0.05). The ALP activity in carp serum was significantly reduced by 30% in the fish fed the diet containing 30% of GMS (P<0.05). Therefore, adding a high amount of glyphosate-resistant GMS to the diet will significantly affect the antioxidant capacity of the hepatopancreatic of Jian carp, and have a certain degree of negative impact on the immunity of the carp. However, whether the critical cause of these effects is genetically modified or not needs to be further explored.
[1]OLSEN R L, HASAN M R. A limited supply of fishmeal:impact on future increases in global aquaculture production[J].Trends in Food Science & Technology,2012,27(2):120-128. [2]东莞林氏.2019年鱼粉行情回顾及2020年形势展望[J].广东饲料,2019,28(12):20-22. [3]国际农业生物技术应用服务组织.2016年全球生物技术/转基因作物商业化发展态势[J].中国生物工程杂志,2017,37(4):1-8. [4]朱元招,王凤来,尹靖东.抗草甘膦大豆及豆粕营养成分和抗营养因子研究[J].营养学报,2010,32(2):178-182. [5]JOBLING M. National Research Council (NRC):nutrient requirements of fish and shrimp[J].Aquaculture International,2012,20(3):601-602. [6]周红蕾,李春玲,王贵平,等.大豆中抗营养因子及其去除方法概述[J].饲料工业,2006,27(3):23-26. [7]HAMMOND B G, VICINI J L, HARTNELL G F, et al. The feeding value of soybeans fed to rats, chickens, catfish and dairy cattle is not altered by genetic incorporation of glyphosate tolerance[J].The Journal of Nutrition,1996,126(3):717-727. [8]SANDEN M, BRUCE I J, RAHMAN M A, et al. The fate of transgenic sequences present in genetically modified plant products in fish feed, investigating the survival of GM soybean DNA fragments during feeding trials in Atlantic salmon, Salmo salar L.[J].Aquaculture,2004,237(1/2/3/4):391-405. [9]SANDEN M, BERNTSSEN M H G, KROGDAHL Å, et al. An examination of the intestinal tract of Atlantic salmon, Salmo salar L., parr fed different varieties of soy and maize[J].Journal of Fish Diseases,2005,28(6):317-330. [10]SANDEN M, KROGDAHL Å, BAKKE-MCKELLEP A M, et al. Growth performance and organ development in Atlantic salmon, Salmo salar L. parr fed genetically modified (GM) soybean and maize[J].Aquaculture Nutrition,2006,12(1):1-14. [11]HEMRE G I, SANDEN M, BAKKE-MCKELLEP A M, et al. Growth, feed utilization and health of Atlantic salmon Salmo salar L. fed genetically modified compared to non-modified commercial hybrid soybeans[J].Aquaculture Nutrition,2005,11(3):157-167. [12]SUHARMAN I, SATOH S, HAGA Y, et al. Utilization of genetically modified soybean meal in Nile tilapia Oreochromis niloticus diets[J].Fisheries Science,2009,75(4):967-973. [13]CHAINARK P, SATOH S, HINO T, et al. Availability of genetically modified soybean meal in rainbow trout Oncorhynchus mykiss diets[J].Fisheries Science,2006,72(5):1072-1078. [14]陶冉.饲料中转基因成分检测及对水产动物安全性评价的初步研究[D].青岛:中国科学院研究生院(海洋研究所),2008:50-52. [15]刘梅,陶冉,王雷,等.进口转基因大豆(Roundup Ready)对吉富罗非鱼生长和生理的影响[J].粮食与饲料工业,2009(2):43-45. [16]刘晓庆. 热处理的转基因和非转基因大豆对不同食性鱼类的影响[D].合肥:安徽大学,2013:34-37. [17]李亚男,张海滨.海洋无脊椎动物抗氧化酶研究进展[J].海洋通报,2018,37(3):241-253. [18]NORDBERG J, ARNÉR E S J. Reactive oxygen species, antioxidants, and the mammalian thioredoxin system 1[J].Free Radical Biology and Medicine,2001,31(11):1287-1312. [19]徐志远.转Cry1Ac/sck基因糙米作为鲤鱼(Cyprinus carpio)日粮原料的安全性评价[D].北京:中国农业科学院,2011:23-33. [20]宋志明,刘鉴毅,庄平,等.低温胁迫对点篮子鱼幼鱼肝脏抗氧化酶活性及丙二醛含量的影响[J].海洋渔业,2015,37(2):142-150. [21]林浩然.鱼类生理学[M].广州:中山大学出版社,2011:105-114. [22]史宗勇,许冬梅,路超,等.短期喂食转基因大豆对雄性大鼠睾丸StAR表达的影响[J].食品与机械,2016,32(10):12-16. [23]杜浩,危起伟,甘芳,等.美洲鲥应激后皮质醇激素和血液生化指标的变化[J].动物学杂志,2006,41(3):80-84. [24]彭翔,宋文新,周凡,等.发酵豆粕替代鱼粉对黑鲷胃肠道和血清指标的影响[J].江苏农业学报,2012,28(5):1096-1103. [25]李秀玲,刘宝锁,张楠,等.发酵豆粕替代鱼粉对卵形鲳鲹生长和血清生化的影响[J].南方水产科学,2019,15(4):68-75. [26]明建华,谢骏,徐跑,等.大黄素、维生素C及其配伍对团头鲂生长、生理生化指标、抗病原感染以及两种HSP70s mRNA表达的影响[J].水产学报,2010,34(9):1447-1459. [27]EISSA M I, EL-SHERBINY M A, IBRAHIM A M, et al. Biochemical and histopathological studies on female and male Wistar rats fed on genetically modified soybean meals (Roundup Ready)[J].The Journal of Basic and Applied Zoology,2019,80(1):54. [28]刘莎莎,谭建庄,孙哲,等.不同水平抗草甘膦转基因豆粕日粮对AA肉仔鸡免疫功能的影响[J].中国畜牧杂志,2011,47(13):41-46. [29]鞠婷婷,占秀安.溶菌酶生物学功能及在动物生产中应用[J].中国畜牧杂志,2016,52(15):79-82. [30]BAKKE-MCKELLEP A M, KOPPANG E O, GUNNES G, et al. Histological, digestive, metabolic, hormonal and some immune factor responses in Atlantic salmon, Salmo salar L., fed genetically modified soybeans[J].Journal of Fish Diseases,2007,30(2):65-79. [31]王崇,雷武,解绶启,等.饲料中豆粕替代鱼粉蛋白对异育银鲫生长、代谢及免疫功能的影响[J].水生生物学报,2009,33(4):740-747. [32]刘勇,冷向军,李小勤,等.豆粕替代鱼粉对奥尼罗非鱼生长、表观消化率及血清非特异性免疫的影响[J].中国粮油学报,2009,24(12):95-100. [33]刘胜军.高温高湿环境对生长猪氧化还原状态和免疫功能的影响[D].北京:中国农业科学院,2010:8-10. [34]高珊,余涛,周景祥,等.鱼类白介素及其受体的研究[J].水产学杂志,2014,27(3):62-64. [35]田佳音,陈康勇,张畅,等.斑马鱼肿瘤坏死因子(tnfα)及其受体(tnfrsf1a)应答细菌和病毒感染的表达[J].上海海洋大学学报,2020,29(2):171-179. [36]谭建庄.抗草甘膦转基因豆粕对肉仔鸡的饲用安全性评定[D].北京:中国农业科学院,2011:25-26. [37]FINAMORE A, ROSELLI M, BRITTI S, et al. Intestinal and peripheral immune response to MON810 maize ingestion in weaning and old mice[J].Journal of Agricultural and Food Chemistry,2008,56(23):11533-11539.
2023, Vol. 42 
