Molecular Phylogenetic Relationships among 16 Species of Sweetlips Based on 3 Gene Sequences
CHENG Jing1, ZHANG Guixin2, CHEN Houhua2, YANG Sen2, LI Peiyuan2, CHEN Jingyi2, LIANG Rishen2
1. Shanghai Vocational College of Agriculture and Forestry, Shanghai 201600, China; 2. College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
Abstract:A total of 16 species of sweetlips were obtained, 14 species belonged to the genus Plectorhinchus, 2 species belonged to the genus Diagramma, domestic samples from the South China Sea and surrounding seas, while most of the foreign samples from the Indian Ocean, one in the South Pacific, and the mitochondrial Cyt b and nuclear RAG2 and S7 labeling sequences of these 16 fish species were obtained by PCR amplification and sequencing. The phylogenetic relationship of analytical molecules was constructed based on maximum likelihood method and Bayesian method. The results showed that the homologous sequence of 16 sweetlips in Cyt b genes were determined to be 657 bp, encoding 219 amino acids; the homologous sequences in RAG2 gene were 726 bp, encoding 243 amino acids, the homologous sequence in S7 genes were 729 bp, with numerous base insertions and deletions. The molecular phylogenetic tree were constructed using Pomadasys maculatus and P. hasta as outgroup species, sweetlips in the tree were divided into three major groups. Species in group Ⅰ possessed spectacular coloration and markings along bodies while species in group Ⅱ and group Ⅲ had monotone color and uniformly dark patterns, which were consistent with the morphological classification. Genus Diagramma did not form an independent branch but were clustered inside the Plectorhinchus group. Comparison of the dorsal spine numbers of Diagramma and Plectorhinchus with their relationships in the phylogenetic tree revealed that no correlation was found between dorsal spine numbers and species phylogenetic relationship, indicating that numbers of dorsal spines was used as an indicator to distinguish different sweetlips morphologically, but not be used as a basis to investigate the their relationships. The findings supported the viewpoints of most current molecular phylogenetic studies that genus Diagramma was classified into genus Plectorhinchus.
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