Abstract:Under the conditions of water temperature of (22±1) ℃, pH 8.0—8.5 and dissolved oxygen level of 7—8 mg/L, 20 μL of microcystins with the concentration of 3.52×10-4 mmol/kg was injected into Pacific white shrimp Litopenaeus vannamei with body weight of (16.0±1.0) g. The mortality of Pacific white shrimp was measured at 0, 2, 4, 8, 16, 24, 48 and 72 hours after injection, and hepatopancreas tissues were taken at the same time. The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) and the changes in malondialdehyde (MDA) content were detected to study the effects of microcystins on the hepatopancreas of Pacific white shrimp. The results showed that the 72 h mortality rate was 13.33% in Pacific white shrimp after injection of microcystins. The content of malondialdehyde in hepatopancreas of Pacific white shrimp was increased gradually after injection of microcystins, which was significantly higher than that in the control group after 8 h, and reached the maximum value of (4.02±0.02) nmol/mg at 72 h (P<0.05). The activities of SOD, CAT and GSH-Px all showed a trend of increasing at first and then decreasing within 72 h, with the maximum at 2,4 and 8 h, respectively. Then the activity was gradually decreased, which was lower than that in the control group after 16 h, with the minimal value at 72 h. The findings indicated that microcystins gradually enhanced the lipid peroxidation in the hepatopancreas of Pacific white shrimp, inhibited the activities of antioxidant-related enzymes in Pacific white shrimp, and caused oxidative stress, causing severe oxidative damage to the hepatopancreas within 72 hours.
[1]粟丽,朱长波,张汉华,等.混养罗非鱼对虾池浮游微藻群落的影响及浮游微藻优势种组成与罗非鱼生长的关系[J].广东农业科学,2011,38(14):101-104. [2]丁笑生,李效宇.微囊藻毒素的研究进展[J].水产科学,2004,23(12):42-43. [3]CHEN L, ZHANG X Z, ZHOU W S, et al. The interactive effects of cytoskeleton disruption and mitochondria dysfunction lead to reproductive toxicity induced by microcystin-LR[J].PLoS One,2013,8(1):e53949. [4]ZHOU M, TU W W, XU J. Mechanisms of microcystin-LR-induced cytoskeletal disruption in animal cells[J].Toxicon,2015,101:92-100. [5]SYMONDS H, KRALL L, REMINGTON L, et al. p53-Dependent apoptosis suppresses tumor growth and progression in vivo[J].Cell,1994,78(4):703-711. [6]贺燕,黄先智,丁晓雯.微囊藻毒素毒性及其作用机理研究进展[J].食品科学,2020,41(5):290-298. [7]丁笑生,段红英.微囊藻毒素及其生殖毒性的研究进展[J].水产科学,2007,26(1):61-62. [8]原居林,叶金云,沈锦玉,等.微囊藻毒素对太湖翘嘴红鲌早期发育阶段的毒性研究[J].水产科学,2010,29(6):334-338. [9]BIECZYNSKI F, DE ANNA J S, PIREZ M, et al. Cellular transport of microcystin-LR in rainbow trout (Oncorhynchus mykiss) across the intestinal wall:possible involvement of multidrug resistance-associated proteins[J].Aquatic Toxicology,2014,154:97-106. [10]SVIREV Z, DROBAC D, TOKODI N, et al. Epidemiology of primary liver cancer in Serbia and possible connection with cyanobacterial blooms[J].Journal of Environmental Science and Health Part C,2013,31(3):181-200. [11]张春景,陆开宏,朱津永,等.微囊藻毒素对鱼类组织器官的毒理学研究进展[J].水产科学,2009,28(9):537-541. [12]CHEN J, XIE P. Tissue distributions and seasonal dynamics of the hepatotoxic microcystins-LR and-RR in two freshwater shrimps, Palaemon modestus and Macrobrachium nipponensis, from a large shallow, eutrophic lake of the subtropical China[J].Toxicon,2005,45(5):615-625. [13]SDERHLL K, CERENIUS L. Role of the prophenoloxidase-activating system in invertebrate immunity[J].Current Opinion in Immunology,1998,10(1):23-28. [14]CHEN L, XIE P. Mechanisms of microcystin-induced cytotoxicity and apoptosis[J].Mini Reviews in Medicinal Chemistry,2016,16(13):1018-1031. [15]曹清晟,王丽萍,杨辉,等.低剂量微囊藻毒素MC-LR诱导罗氏沼虾肝胰腺损伤及凋亡[J].生态毒理学报,2020,15(2):171-179. [16]JIANG J L, SHAN Z J, XU W L, et al. Microcystin-LR induced reactive oxygen species mediate cytoskeletal disruption and apoptosis of hepatocytes in Cyprinus carpio L.[J].PLoS One,2013,8(12):e84768. [17]MUOZ M, CEDEO R, RODRíGUEZ J, et al. Measurement of reactive oxygen intermediate production in haemocytes of the penaeid shrimp, Penaeus vannamei[J].Aquaculture,2000,191(1/2/3):89-107. [18]张榜军.微囊藻毒素LR对小鼠的肝毒性及其分子机理研究[D].新乡:河南师范大学,2016. [19]隗黎丽,何丽,阮记明,等.草鱼幼鱼肝胰脏抗氧化系统对MC-LR胁迫的响应[J].农业环境科学学报,2019,38(1):44-50. [20]JIANG J L, SHI Y, SHAN Z J, et al. Bioaccumulation, oxidative stress and HSP70 expression in Cyprinus carpio L. exposed to microcystin-LR under laboratory conditions[J].Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology,2012,155(3):483-490. [21]PINHO G L L, ROSA C M D, YUNES J S, et al. Toxic effects of microcystins in the hepatopancreas of the estuarine crab Chasmagnathus granulatus (Decapoda, Grapsidae)[J].Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology,2003,135(4):459-468. [22]BIECZYNSKI F, BIANCHI V A, LUQUET C M. Accumulation and biochemical effects of microcystin-LR on the Patagonian pejerrey (Odontesthes hatcheri) fed with the toxic cyanobacteria Microcystis aeruginosa[J].Fish Physiology and Biochemistry,2013,39(5):1309-1321. [23]CAZENAVE J, BISTONI M D L A, PESCE S F, et al. Differential detoxification and antioxidant response in diverse organs of Corydoras paleatus experimentally exposed to microcystin-RR[J].Aquatic Toxicology,2006,76(1):1-12. [24]PAVAGADHI S, GONG Z Y, HANDE M P, et al. Biochemical response of diverse organs in adult Danio rerio (zebrafish) exposed to sub-lethal concentrations of microcystin-LR and microcystin-RR:a balneation study[J].Aquatic Toxicology,2012,109:1-10. [25]GONALVES-SOARES D, ZANETTE J, YUNES J S, et al. Expression and activity of glutathione S-transferases and catalase in the shrimp Litopenaeus vannamei inoculated with a toxic Microcystis aeruginosa strain[J].Marine Environmental Research,2012,75:54-61. [26]陈妍妍.虾池蓝藻群落及微囊藻毒素对凡纳滨对虾相关免疫酶影响[D].湛江:广东海洋大学,2016:34-36. [27]陈隽.肝毒性微囊藻毒素在巢湖和太湖水生动物体内的生物富集及对水产品安全性的潜在威胁[D].武汉:中国科学院研究生院(水生生物研究所),2006:46-51. [28]张大文,袁丽娟,张莉,等.微囊藻毒素-LR对鸡肝脏的氧化损伤影响[J].生态毒理学报,2019,14(5):267-275. [29]李智,傅一鸣,李响,等.凡纳滨对虾在微囊藻毒素(MC-LR)诱导下3种抗菌肽基因的表达量分析[J].上海海洋大学学报,2014,23(6):842-847. [30]查广才,周昌清,牛晓光.铜绿微囊藻对凡纳滨对虾低盐度养殖的危害研究[J].中山大学学报(自然科学版),2007,46(2):64-67. [31]FISCHER A, HOEGER S J, STEMMER K, et al. The role of organic anion transporting polypeptides (OATPs/SLCOs) in the toxicity of different microcystin congeners in vitro:a comparison of primary human hepatocytes and OATP-transfected HEK293 cells[J].Toxicology and Applied Pharmacology,2010,245(1):9-20. [32]胡益鸣,李琪,刘士凯,等.温度和盐度急性胁迫对岩牡蛎存活及免疫指标的影响[J].中国水产科学,2020,27(3):286-294. [33]梁花蕾.饲料中添加蓝藻粉对鲫肌肉品质、脂肪代谢的影响[D].武汉:华中农业大学,2016:45-46. [34]赵伟,王宝杰,刘梅,等.短期饲喂黄曲霉毒素B1对凡纳滨对虾肝胰腺抗氧化酶活性及基因表达的影响[J].饲料工业,2017,38(8):23-28. [35]WANG W N, ZHOU J, WANG P, et al. Oxidative stress, DNA damage and antioxidant enzyme gene expression in the Pacific white shrimp, Litopenaeus vannamei when exposed to acute pH stress[J].Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology,2009,150(4):428-435. [36]许燕,杨洁,孙静秋,等.凡纳滨对虾不同组织内SOD、POD酶的细胞化学定位[J].水生生物学报,2010,34(2):402-409. [37]KANG S W, RHEE S G, CHANG T S, et al. 2-Cys peroxiredoxin function in intracellular signal transduction:therapeutic implications[J].Trends in Molecular Medicine,2005,11(12):571-578. [38]BAUER G. Tumor cell-protective catalase as a novel target for rational therapeutic approaches based on specific intercellular ROS signaling[J].Anticancer Research,2012,32(7):2599-2624. [39]姜招峰,杨翰仪.氧自由基对CAT、SOD和GPX的氧化修饰研究[J].北京联合大学学报(自然科学版),2003,17(3):12-17. [40]SCHWARZ K B. Oxidative stress during viral infection:a review[J].Free Radical Biology and Medicine,1996,21(5):641-649. [41]侯杰.微囊藻毒素-LR对斑马鱼生殖和生长发育的影响及其机制[D].武汉:华中农业大学,2017:36-37.