Heritability Assessment of High Temperature Tolerance Traits in Juvenile Pacific White Shrimp Litopenaeus vannamei
QU Min1,2, TAN Jian2,3, LUAN Sheng2,3, QIANG Guangfeng2,3, LUO Kun2,3, SUI Juan2,3, MENG Xianhong2,3, KONG Jie2,3
1. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China; 2. Key Laboratory of Marine Fisheries Sustainable Development, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; 3. Functional Laboratory of Marine Fishery Science and Food Production Processes, Qingdao Pilot National Laboratory of Marine Science and Technology, Qingdao 266071, China
Abstract:In order to study the effects of high temperature on genetic variation of Pacific white shrimp Litopenaeus vannamei, 40 full-sibling families were randomly selected from the 4 populations of Pacific white shrimp (EGDB, PRMA, API, and SIS) that were introduced from abroad in 2018, and 100 individuals were randomly selected from each family, divided into two parts and reared to P21—P23 in the designated test barrels. The test barrels were heated using a water bath heating method and its corresponding survival time and temperature were recorded. A single character animal model was used to evaluate the genetic parameters of its heat resistance. The results showed that the heat resistance values of larvae of each family were ranged from 782.64 to 2074.33 ℃·h, indicating that there were significant differences in heat resistance among Pacific white shrimp larvae. The coefficient of variation of heat resistance between families was ranged from 10% to 68%, and the cumulative survival rate of each line was ranged from 58% to 99% at water temperature increasing from 28 ℃ to 40 ℃. The heritability of heat resistance traits in juvenile Pacific white shrimp was estimated to be 0.22±0.05, as medium heritability, by using a single character animal model combined with pedigree information. There was a high level of genetic variation in heat resistance traits of juvenile Pacific white shrimp, and there was a great potential for genetic improvement. The findings can provide data reference for high- temperature culture and breeding of high-temperature resistant strains of Pacific white shrimp in summer.
[1] 黄永春,郑伟刚,陈辉辉,等.凡纳滨对虾2个选育群体的生长、消化、免疫酶活性及其抗白斑综合症病毒性能[J].厦门大学学报(自然科学版),2017,56(2):188-193. [2] 黄小帅,徐煜,胡晓娟,等.利用微卫星标记分析7个凡纳滨对虾引进群体子一代的遗传多样性[J].南方水产科学,2019,15(1):54-62. [3] 农业农村部渔业渔政管理局,全国水产技术推广总站,中国水产学会.2020中国渔业统计年鉴[M].北京:中国农业出版社,2020. [4] KUMLU M, KUMLU M, TURKMEN S. Combined effects of temperature and salinity on critical thermal minima of Pacific white shrimp Litopenaeus vannamei (Crustacea:Penaeidae)[J].Journal of Thermal Biology,2010,35(6):302-304. [5] 卓晓菲,何苹萍,韦嫔媛,等.凡纳滨对虾低温差异表达miRNA的分析鉴定及靶基因的验证[J].水生生物学报,2019,43(6):1203-1209. [6] 蒋天宝.常见理化因子对凡纳滨对虾生长存活的影响研究概况[J].当代水产,2015,40(9):92-95. [7] 翟盘茂,刘静.气候变暖背景下的极端天气气候事件与防灾减灾[J].中国工程科学,2012,14(9):55-63. [8] 王吉桥,罗鸣,张德治,等.水温和盐度对南美白对虾幼虾能量收支的影响[J].水产学报,2004,28(2):161-166. [9] 孙金辉,徐赟霞,季延滨,等.温度骤降对南美白对虾仔虾抗氧化机能的影响[J].天津农学院学报,2008,15(3):7-10. [10] 谢数涛,吴任,许忠能,等.亚致死温度热休克对凡纳滨对虾的保护作用[J].中山大学学报(自然科学版),2005,44(6):87-89. [11] 李丽君,宋晓红,林瑶琼,等.日本囊对虾耐热性状及其与凡纳滨对虾、脊尾白虾的比较[J].中国水产科学,2015,22(3):418-425. [12] 陈文静,裴建明,邓勇辉,等.罗氏沼虾蚤状幼体对温度、盐度、pH值、溶解氧、亚硝酸盐和氨的适应范围试验[J].江西水产科技,2001(3):20-25. [13] 李莹春,朱国平,孟涛,等.人工条件下南极磷虾的温度耐受性试验观察[J].江苏农业科学,2012,40(9):204-206. [14] ALLAN E L, FRONEMAN P W, HODGSON A N. Effects of temperature and salinity on the standard metabolic rate (SMR) of the caridean shrimp Palae-mon peringueyi[J].Journal of Experimental Marine Biology and Ecology,2006,337(1):103-108. [15] 胡利杰,李旭鹏,孟宪红,等.凡纳滨对虾6-磷酸海藻糖合成酶在抗高温胁迫中的表达特征[J].渔业科学进展,2020,41(2):159-167. [16] LI W J, LUAN S, LUO K, et al. Genetic parameters and genotype by environment interaction for cold tolerance, body weight and survival of the Pacific white shrimp Penaeus vannamei at different temperatures[J].Aquaculture,2015,441:8-15. [17] 刘宝锁,张天时,孔杰,等.大菱鲆生长和耐高温性状的遗传参数估计[J].水产学报,2011,35(11):1601-1606. [18] 孔杰,栾生,谭建,等.对虾选择育种研究进展[J].中国海洋大学学报(自然科学版),2020,50(9):81-97. [19] 张沛东,张秀梅,李健.对虾行为生态学研究进展Ⅱ.环境因子对对虾行为习性的影响[J].应用生态学报,2006,17(2):340-344. [20] 李东宇,孟宪红,孔杰,等.凡纳滨对虾(Litopenaeus vannamei)选育群体与杂交群体遗传多样性差异及其在低温条件下生长性能的比较[J].渔业科学进展,2017,38(4):69-77. [21] 杨章武,张哲,陈思源,等.凡纳滨对虾选育群体子代仔虾幼体耐低温能力比较研究[J].渔业研究,2020,42(3):253-260. [22] 程光平,桑明远,黄国强,等.不同低温持续胁迫对凡纳滨对虾存活的影响[J].南方农业学报,2013,44(2):333-337. [23] 朱华平,卢迈新,黄樟翰,等.水产动物选择育种的常用方法和应用[J].海洋与渔业,2010(2):48-49. [24] 唐扬,孟小菲,沈瑞福,等.凡纳滨对虾家系选育的研究与应用[J].水产科学,2018,37(4):555-563. [25] HENRYON M, JOKUMSEN A, BERG P, et al. Genetic variation for growth rate, feed conversion efficiency, and disease resistance exists within a farmed population of rainbow trout[J].Aquaculture,2002,209(1/2/3/4):59-76. [26] FRIARS G W, MCMILLAN I, QUINTON V M, et al. Family differences in relative growth of diploid and triploid Atlantic salmon (Salmo salar L.)[J].Aquaculture,2001,192(1):23-29. [27] 陈松林,田永胜,徐田军,等.牙鲆抗病群体和家系的建立及其生长和抗病性能初步测定[J].水产学报,2008,32(5):665-673. [28] 曾凡勇,罗坤,栾生,等.凡纳滨对虾在氯化物型盐碱水养殖环境下不同家系间生长、存活性能分析[J].中国水产科学,2018,25(2):308-315. [29] 王军.基于SSR标记的中国对虾抗WSSV性状遗传参数估计及野生和选育群体的遗传多样性分析[D].上海:上海海洋大学,2017:8-9. [30] 董丽君.对虾耐低温性状遗传参数分析及相关基因筛选[D].上海:上海海洋大学,2018:2-3. [31] 王明珠,孟宪红,孔杰,等.低温胁迫条件下中国明对虾生长性状和耐低温性状的遗传参数评估[J].渔业科学进展,2018,39(3):96-102.