Effect of Low Temperature Stress on Function of Thymosins Gene in Pacific White Shrimp Litopenaeus vannamei
ZHANG Yi1, JIN Kuifeng1, LI Wenhui1, YANG Yingcan1, LAI Hui1,2, LIU Wenguang3
1. Meteorological Public Service Center of Guangdong Province, Guangzhou 510640, China; 2. Guangdong HuayunTechnology Development Company, Guangzhou 510640, China; 3. South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
Abstract：In order to investigate the molecular mechanism of thymosinsgene under low temperature stress in Pacific white shrimp Litopenaeus vannamei, the Lv-thymosins gene was cloned by rapid amplification of cDNA ends (RACE), and then the tissue distribution and the expression profiles of Lvthy gene under low temperature stress was detected by real-time quantitative PCR. Subsequently, RNA interference (RNAi) experiment was conducted to be knocked down the expression of Lvthy gene to verify the gene function under low temperature stress. It was found that the full length of the Lvthy gene cDNA sequences consisted of 1765 base pairs (bp), including a 729 bp open reading frame (ORF) encoding a 242-amino acid peptide with six thymosins beta actin-binding motif. The qRT-PCR showed that Lvthy gene was expressed in all tissues of the Pacific white shrimp, the maximal expression level in stomach, followed by intestine, heart and brain. Low temperature challenge showed that the expression level of the Lvthy gene was shown to be increased 1 h post low temperature stimulation. The maximal level (2.7 times compared with the control group) was found at 3 h in the shrimp challenged and then to be declined. RNA interference (RNAi) technology verified the gene function. The survival rate of the Pacific white shrimp was 31.1% under low temperature stress, significantly lower than that in the control group (83.3%). The findings indicate that the Lvthy gene plays critical role in the process of accumulating to cold stress and that provide a foundation for the study of adaptation mechanism of crustaceans under low temperature stress.
张毅, 靳奎峰, 李文慧, 杨颖璨, 来辉, 刘文广. 低温胁迫对凡纳滨对虾胸腺肽基因功能的影响[J]. 水产科学, 2024, 43(1): 104-110.
ZHANG Yi, JIN Kuifeng, LI Wenhui, YANG Yingcan, LAI Hui, LIU Wenguang. Effect of Low Temperature Stress on Function of Thymosins Gene in Pacific White Shrimp Litopenaeus vannamei. 水产科学, 2024, 43(1): 104-110.
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2024, Vol. 43 
