Abstract：In order to understand the physiological characteristics of sperm of fat minnow Phoxinus lagowskii and improve its fertilization rate, we studied the effects of metal ion concentrations, temperature and pH on the sperm motility of fat minnow.The sperm fast moving time (FT) and life time (LT) were measured of 10 individuals of male fat minnow (body weight of 80.0—100.0 g and body length of 10.0—20.0 cm) under different ions [Na+, K+, Ca2+, glucose, and Tris buffer (C4H11NO3)], different temperatures [(8.0±1.0) ℃, (12.0±1.0) ℃, (16.0±1.0) ℃, (20.0±1.0) ℃, (24.0±1.0) ℃, and (30.0±1.0) ℃] and different pH (5.0, 6.0, 7.0, 8.0, and 9.0). The results showed that the sperm had the FT of (72.05±9.93) s and rising rate of 28.1%, the LT of (106.86±6.00) s with rising rate of 39.4%at Na+ concentration of 100 mmol/L. The sperm motility was the strongest at the K+ concentration of 75 mmol/L, and had the FT of (59.47±5.76) s with rising rate of 5.7%, and the LT of (252.11±218.64) s with rising rate of 228.9%, without no significant difference in the FT between 0 and 100 mmol/L glucose concentrations, but increase in LT with the increase in glucose concentration. In addition, Ca2+ and Tris buffer inhibited the sperm motility, with short FT and LT. The sperm motility was better with FT of (32.99±7.50) s and LT of(78.77±8.47) s at temperature of (20.0±1.0) ℃. The longest FT and LT was observed at pH 8.0, (45.42±6.56) s and (475.02±46.35) s. Without significant difference in the time of sperm rapid movement(745.67±273.08) s in compound solution B [V(Na+100 mmol/L)∶V(K+75 mmol/L)=1∶1],significantly higher life time than that in other conditions, with an increase rate of 968.6%. It was found that appropriate Na+, K+ and glucose concentration promoted the sperm motility of fat minnow, Ca2+ and that Tris buffer inhibited the sperm motility. The best sperm motility was observed in the fat minnow exosed to water temperature of (20.0±1.0) ℃ and pH range of 7.0—8.0. Compound solution B [V(Na+ 100 mmol/L)∶V(K+ 75 mmol/L)=1∶1] led to significant increase in sperm longevity.
吴晨, 骆小年, 段友健, 曾永聪, 缪云亮, 韩峰, 罗超. 不同离子浓度、温度、pH对拉氏鱼岁精子活力的影响[J]. 水产科学, 2022, 41(2): 210-217.
WU Chen, LUO Xiaonian, DUAN Youjian, ZENG Yongcong, MIAO Yunliang, HAN Feng, LUO Chao. Effects of Different Metal Concentrations, Temperature and pH on Sperm Motility of Fat Minnow Phoxinus lagowskii. Fisheries Science, 2022, 41(2): 210-217.
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2022, Vol. 41 
