Effects of Light Spectrum on Intestinal Microbial Composition of Juvenile Tiger Puffer Takifugu rubripes
LU Hongbo1, LIU Ying1,2, SHEN Xufang2,3, WANG Jia1, ZHOU Huiting1, JIANG Jieming1,2, LI Zequn1, LIU Qi2, YAN Hongwei1,2, SUN Qunwen4
1. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China; 2. Key Laboratory ofMinistry of Education for Facility Fisheries, Dalian 116023, China; 3. Liaoning Normal University,Dalian 116029, China; 4. Dalian Tianzheng Industrial Company Limited, Dalian 116036, China
Abstract：In order to explore the influence of different light spectra on the gut microbial composition of tiger puffer Takifugu rubripes, in the experiment the juvenile tiger puffer was taken as the research object. The fertilized eggs were hatched and bred for a total of 90 days under 5 different light spectrum conditions (blue, green, red, white, and yellow). After the feeding trail, the body length of juvenile fish was measured in each group, and the composition of their gut microbes was analyzed using Illumina HiSeq high-throughput sequencing technology. The results showed that the body length of juvenile fish was found to be significantly higher in the green light group, yellow light group and white light groups than that in the blue light group and the red light group (P<0.05 ). The survival rate of each group was from high to low described as blue light group> red light group> yellow light group> white light group> green light group, without significant difference between the groups (P>0.05). A cluster analysis with a similarity of 97% showed that the number of operational taxonomic units (OTUs) of the intestinal microbes in the blue light group, green light group, red light group, white light group, and yellow light group were found to be 631, 508, 530, 513, and 418, respectively, with the total OTUs number of 87, the unique OTUs number of 0, were found to be but the number of OUTs shared by the blue light group and the red light group was 13. The Ace and Chao1 indices of the blue light group were significantly higher in the blue light group than those in the yellow light group (P<0.05), and there was the maximal abundance of microbial flora in the blue light group, while the minimal abundance in the yellow light group. The Shannon index of blue light and red light group was significantly higher than that in green light and yellow light groups (P<0.05 ), that is, the diversity of gut microbial flora in blue light and red light groups was greater than that in green light and yellow light groups. According to the distribution of the intestinal flora at the level of the genus, the genera Arcobacter, Vibrio, mycoplasma, Rhodococcus, Brevinema, Brevibacterium, Cetobacterium, Pseudoalteromonas and Ochrobactrum were the main genus of the intestinal tract of tiger puffer, among which Arcobacter was the absolute dominant bacteria in the intestinal microbe genus. In summary, the spectrum can affect the growth of tiger puffer, and the spectrum has a micro-regulatory effect on the homeostasis of juvenile gut microbes, but the effect on the gut microbial structure of juvenile fish is not obvious.
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2023, Vol. 42 
