Abstract：In order to improve the survival rate in operation and transportation, the anesthetic effect of tricaine and eugenol on barbel Barbodes semifasciolatus with body weight of (0.28±0.09) g acclimated for 2 weeks was investigated under water temperature of (25.0±0.5) ℃: the barbel were exposured to 80, 90, 100, 110, 120, 130, 140, 150, 160 mg/L tricaine and 15, 20, 25, 30 and 35 mg/L eugenol to explore the effective anesthetic dose; the barbel were exposed to tricaine with concentration of 140 mg/L and eugenol with a mass concentration of 25 mg/L after deep anesthesia exposure to water for 0, 2, 4, 6, 8 and 10 min, and then put back into clean water for recovery, recording the recovery time and counting the recovery rate. After exposing the barbel to tricaine with concentration of 10 mg/L and eugenol with concentration of 8 mg/L, the transport test was simulated to explore the application effect of two anesthetics for different operating purposes at room temperature.The results showed that the suitable mass concentrations were 100—140 mg/L for tricaine and 20—30 mg/L for eugenolm. After the deep anesthesia at tricaine mass concentration of 140 mg/L, the suitable exposure time in air was below 6 min, while the barbel was recoveryed to 100% within 8 min of air exposure after deep anesthesia at eugenol mass concentration of 25 mg/L. An anesthesia transport test showed the two anesthetics both had good transport effect within 48 h at fish: water ratio of 1∶15, and tricaine was available at fish: water ratio of 1∶20 under 72 h transportation with the survival rate up to 90.74%. Both tricaine and eugenol had good anaesthetic effects on the barbel in practical applications, were chosen for a suitable anaesthetic mass concentration according to different operating purposes and in combination with other elements.
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2024, Vol. 43 
