Difference Analysis in Intestinal Flora of Juvenile Echiura Worm Urechis unicinctus with Different Growth Rates
DONG Haomiao1,2, WANG Fangyi3, XU Dong4, JIAO Xudong1,2, WANG Weizhong4
1. Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China; 3. Weifang Fisheries Technology Extension Station, Weifang 261000, China; 4. Shandong Blue Ocean Technology Co., Ltd, Yantai 261400, China
Abstract：In order to explore the relationship between growth difference of juvenile echiura worm Urechisunicinctus and its intestinal microbiota with different growth rates, the composition and changes in bacterial flora were analyzed in the intestines especially dissected of the juvenile echiura worm anesthesized by MS-222 with body weight of greater than 700 mg in fast-growth group (FG group) and with body weight of less than 300 mg in slow-growth group (SG group) sampled in three 100 cm2 substrate regions in the same culture pond by 16S rRNA high-throughput sequencing. The results showed that greater diversity of intestinal flora species was found in the juvenile echiura worm in FG group than that in FG group, with dominant bacteria in Proteobacteria and Firmicutes in the intestine. There was the maximal relative abundance of Ralstonia in FG group and significantly higher than that in SG group, while there was the maximal abundance of Candidatus_hepatoplasma in SG group and significantly higher than that in FG group.LEfSe analysis revealed that more Pseudomonas, which was speculated to promote individual growth, was observed in FG group, with more Candidatus_hepatoplasma in SG group, or the marker genus with too slow growth rate. Functional prediction showed that the relative abundance of bacteria involved in cellular processes and signaling was significantly higher in FG group than that in SG group, while the relative abundance of microorganisms involved in cell growth and death function groups accounted for a significant advantage in SG group, indicating that there was a correlation between the growth rate of echiura worm and the intestinal flora. The findings can provide theoretical guidance for the healthy breeding and efficient cultivation of echiura worm.
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