Intestinal Microbiota Analysis in Gibel Carp Carassius auratus gibelio Based on 16S rRNA Gene Sequence
CHEN Peng1,2, SUN Qirui1,2, ZHANG Honghua3, WANG Shuai2, WANG Jiawei2, XU Yang1,2, ZHANG Mingming2, QIAO Guo2, LI Qiang2
1. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China; 2. College of Ocean and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, China; 3. Shanggang Town Agricultural Center of Jianhu County, Yancheng 224731, China
Abstract：Gibel carp Carassius auratus gibelio is the mainly economical farmed fish species in China. In this study, Illumina sequencing and traditional bacterial isolation techniques were combined to analyze the intestinal microbiota of gibel carp to provide a good guidance for sustainable gibel carp culture. Illumina sequencing revealed that the intestinal microbial richness and diversity in wild group did not change significantly compared with the culture group, with significant difference in microbiota structure. At the phylum level, the relative abundance of Firmicutes was significantly higher in the culture group than that in the wild group, while the relative abundance of Proteobacteria was significantly lower than that in the wild group (P<0.05). At the genus level, the relative abundance of Aeromonas, Pseudomonas and Peptostreptococcus was significantly higher in the culture group than that in the wild group (P<0.01), while Rubrobacter was significantly lower than that in the wild group (P<0.01). According to the traditional bacterial isolation and identification analysis, Staphylococcus was the predominant genus in both wild and culture groups, followed by Micrococcus. Additionally, Bacillus was also isolated from the intestine of gibel carp in both groups. There was significantly more bacterial species richness in the wild group than that in culture group. The findings will provide theoretical foundation for sustainable green gibel carp culture.
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2022, Vol. 41 
