Effect of a High Protein Content Compound Feed Substitution for Trash fish on Intestinal Microbiota in Mud Crab Scylla paramamosain
SU Guisen1,2, LI Min1, CHEN Ran1, HU Gaoyu1, XIAO Guoqiang1,2, CAI Jingbo1, ZHANG Xiang1
1. Zhejiang Key Lab of Exploitation and Preservation of Coastal Bio-Resource, Wenzhou Key Laboratory of Marine Genetics and Breeding, Zhejiang Mariculture Research Institute,Wenzhou 325005,China; 2. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
Abstract：To explore the differences of intestinal microbiota for Scylla paramamosain fed different diets (Eel feed-EF, and fresh fish-FF) under crab apartment, the microbial community structure and changes were studied by using the 16S rRNA gene amplicon sequencing technology in this study. The results showed that Proteobacteria, Firmicutes, Fusobacteria, and Bacteroidetes were the four most dominant categories at phylum level whose number of OTU accounted for more than 85.84%. The OTUs of common bacterial community accounted for 18.92% represented 95.39% of the sequences. The microbial community of different diets was no remarkably distinct by PCoA. With the pass of periods, the complexity of bacterial co-occurrence relationship decreased. The function analyses of intestinal microbiota showed that there was significant difference in secondary metabolites biosynthesis, transport and catabolism in early period, which was not significant in late period. Overall EF has no significant effect on the bacterial community structure and function.
苏贵森, 李敏, 陈然, 胡高宇, 肖国强, 蔡景波, 张翔. 高蛋白配合饲料替代饲喂对拟穴青蟹肠道菌群的影响[J]. 水产科学, 2023, 42(6): 955-965.
SU Guisen, LI Min, CHEN Ran, HU Gaoyu, XIAO Guoqiang, CAI Jingbo, ZHANG Xiang. Effect of a High Protein Content Compound Feed Substitution for Trash fish on Intestinal Microbiota in Mud Crab Scylla paramamosain. Fisheries Science, 2023, 42(6): 955-965.
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2023, Vol. 42 
