Safety Assessment and Acute Toxicity of Four Kinds of Heavy Metal Ions to Manila Clam Ruditapes philippinarum
CHEN Zhi1, WEI Chengchen2, HU Fengxiao2, HUANG Jian3, LI Weipeng2, LIAN Chenyang1
1. Freshwater Fishery Research Institute of Fujian Province, Fuzhou 350002, China; 2. Fujian Agriculture and Forestry University, Fuzhou 350002, China; 3. Fisheries and Aquaculture Technology Extension Station of Fujian Province, Fuzhou 350002, China
Abstract：The acute toxic effects of four kinds of heavy metal ions on Manila clam Ruditapes philippinarum were investigated by toxicology experiment, in order to evaluate the tolerance of Manila clam to Cd2+, Mn2+, Zn2+ and Cu2+. The results showed that the mortality of Manila clam was positively proportional to the concentration of heavy metal ions, with significant dose-response relationship. The medium lethal concentration (LC50) values of Cd2+ treatment was 38.62 mg/L for 24 h, 29.83 mg/L for 48 h, 8.11 mg/L for 72 h, and 2.67 mg/L for 96 h with safe concentration of 0.03 mg/L, whereas the LC50 of Mn2+ was 712.58 mg/L for 24 h, 421.57 mg/L for 48 h,188.74 mg/L for 72 h, and 61.27 mg/L for 96 h with safe concentration of 0.61 mg/L. The LC50 of Zn2+ was 141.27 mg/L for 24 h,72.20 mg/L for 48 h, 34.91 mg/L for 72 h and 21.57 mg/L for 96 h with safe concentration of 0.22 mg/L. The LC50 of Cu2+ was 29.88 mg/L for 24 h, 12.06 mg/L for 48 h,3.62 mg/L for 72 h and 0.79 mg/L for 96 h with safe concentration of 0.01 mg/L. The results showed that the toxicity of four heavy metal ions to Manila clam was in the order of Cu2+>Cd2+>Zn2+>Mn2+, the lower safe concentration of Cu2+ than the standard of fishery water quality, so special attention should be paid to nursery and breeding processes.
陈志, 魏成陈, 胡丰晓, 黄健, 李伟鹏, 连晨阳. 4种重金属离子对菲律宾蛤仔的急性毒性及安全评价[J]. 水产科学, 2021, 40(4): 571-576.
CHEN Zhi, WEI Chengchen, HU Fengxiao, HUANG Jian, LI Weipeng, LIAN Chenyang. Safety Assessment and Acute Toxicity of Four Kinds of Heavy Metal Ions to Manila Clam Ruditapes philippinarum. Fisheries Science, 2021, 40(4): 571-576.
[1]陈志,陈启春,黄健,等.福建沿海菲律宾蛤仔工厂化人工育苗技术[J]. 福建农业科技,2019(7):25-28. [2]郭永禄.胶州湾底播增殖菲律宾蛤仔(Ruditapes philippinarum)渔业生物学研究[D].青岛:中国海洋大学,2005. [3]杜睿贤,贾雪峰,林艺佳,等.海洋贝类重金属富集特征及影响因素研究进展[J].中国农学通报,2019,35(11):155-159. [4]夏娜娜,王军,史云娣,等.海洋重金属污染防治的对策研究[J].中国人口·资源与环境,2012,22(S1):343-346. [5]李状状,李霞,王缙云,等.重金属对鱼类细胞系遗传损伤和基因表达的影响[J].水产科学,2018,37(2):278-282. [6]李晓,王颖,吴志宏,等.魁蚶对3种重金属生物富集动力学研究[J].水产科学,2016,35(5):578-582. [7]苑旭洲,崔毅,陈碧鹃, 等. 菲律宾蛤仔对6种重金属的生物富集动力学[J]. 渔业科学进展,2012,33(4):49-56. [8]陈晓聪,张冉,李成华,等. 菲律宾蛤仔(Venerupis philippinarum)对重金属Hg2+的富集及相关生物标记物的识别[J]. 海洋与湖沼,2015,46(4):928-936. [9]乔庆林,姜朝军,徐捷,等. 菲律宾蛤仔养殖水体中4种重金属安全限量的研究[J]. 浙江海洋学院学报(自然科学版),2006,25(1):5-9. [10]乔庆林,姜朝军,徐捷,等. 双壳贝类养殖水体中Hg、Pb、Cd安全限量的研究[J]. 食品科学,2007,28(3):38-41. [11]宫春光,殷蕊,孙桂清,等. 重金属Hg2+对菲律宾蛤仔的急性毒性及肝脏组织结构的影响[J]. 科学养鱼,2016(7):50-51. [12]张宜奎,宋秀凯,刘爱英,等. 重金属Cd2+、Cu2+对文蛤的急性毒性[J]. 海洋湖沼通报,2011(3):51-56. [13]Leung P T Y, Wang Y, Mak S S T, et al. Differential proteomic responses in hepatopancreas and adductor muscles of the green-lipped mussel Perna viridis to stresses induced by cadmium and hydrogen peroxide[J].Aquatic Toxicology,2011,105(1/2):49-61. [14]Wu H F, Ji C L, Wang Q, et al. Manila clam Venerupis philippinarum as a biomonitor to metal pollution[J].Chinese Journal of Oceanology and Limnology,2013,31(1):65-74. [15]陈晓丹,冼健安,苟妮娜,等. 锰对水产养殖动物生长、免疫与抗应激的影响[J]. 海洋科学,2010,34(10):108-113. [16]唐婉琴,张江惠,袁伦强.水体锰暴露对草鱼碱性磷酸酶、酸性磷酸酶及代谢率的影响[J].重庆师范大学学报(自然科学版),2018,35(3):69-74. [17]赵振山,徐毅刚,王寿兵,等.团头鲂配合饲料中锰和钴添加量的研究[J].水利渔业,1995(1):22-24. [18]蒋蓉,叶元土,蔡春芳,等. 铜、铁、锰、锌组合对黄颡鱼部分生理机能的影响[J]. 饲料博览,2009(9):31-35. [19]王宏伟,郭立格,赵建华,等. 饲料中锰对中华米虾SOD活性的影响[J]. 河北大学学报(自然科学版),2006,26(6):649-652. [20]鲁耀鹏,陈晓丹,张秀霞,等. 饵料锰含量对凡纳滨对虾亚成虾生长及抗氧化活力的影响[J]. 南方农业学报,2018,49(2):391-396. [21]Huang W, Cao L, Shan X J, et al. Toxic effects of zinc on the development,growth,and survival of red sea bream Pagrus major embryos and larvae[J].Archives of Environmental Contamination and Toxicology,2010,58(1):140-150. [22]刘浩明,董迎辉,霍礼辉,等.Cu2+对缢蛏稚贝的急性毒性及对抗氧化酶活力和丙二醛含量的影响[J]. 中国水产科学,2012,19(1):182-187. [23]Papadimitriou E, Loumbourdis N S. Exposure of the frog Rana ridibunda to copper:impact on two biomarkers,lipid peroxidation,and glutathione[J].Bulletin of Environmental Contamination and Toxicology,2002,69(6):885-891. [24]Grosell M, McDonald M D, Wood C M, et al. Effects of prolonged copper exposure in the marine gulf toadfish (Opsanus beta)[J].Aquatic Toxicology,2004,68(3):249-262. [25]秦华伟,刘爱英,谷伟丽,等. 6种重金属对3种海水养殖生物的急性毒性效应[J]. 生态毒理学报,2015,10(6):287-296. [26]高淑英,邹栋樑. 镉、锌和锰对长毛对虾幼体的急性毒性[J]. 海洋通报,1995,14(6):83-86. [27]宁军号,白伟,宋坚,等. 4种重金属离子对偏顶蛤的急性毒性效应[J]. 大连海洋大学学报,2016,31(3):290-294.
2021, Vol. 40 
